Restriction map for
pF1KB3626
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AflIII AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AvrII BbeI BbrPI BcgI BciVI
BclI BlnI BlpI Bpu1102I BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstZ17I Bsu36I
CelII ClaI DrdI EagI EclXI
Eco52I Eco81I EcoRV FbaI FseI
HincII HindII HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 3187 GACGTC GACGT^C
AccI 1699 GTCTAC GT^[AC][GT]AC
AccII 1975 CGCG CG^CG
AclI 925 AACGTT AA^CGTT
Aor51HI 2615 AGCGCT AGC^GCT
AseI 2533 ATTAAT AT^TAAT
AsnI 2533 ATTAAT AT^TAAT
AspI 2125 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1643 CCCGAG C^[CT]CG[GA]G
AviII 1592 TGCGCA TGC^GCA
BanI 1468 GGTGCC G^G[CT][GA]CC
BbvCI 1392 GCTGAGG CCTCAGC(-5/-2)
BglI 138 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2780 AGATCT A^GATCT
BsaAI 1387 TACGTG [CT]AC^GT[GA]
BsaBI 1252 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1725 CGATCG CG[GA][CT]^CG
BsmI 2251 GAATGC GAATGC(1/-1)
BsoBI 1643 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1333 TGTACA T^GTACA
BsrDI 29 GCAATG GCAATG(2/0)
BsrFI 2423 GCCGGT [GA]^CCGG[CT]
BsrGI 1333 TGTACA T^GTACA
BstBI 909 TTCGAA TT^CGAA
BstEII 2746 GGTAACC G^GT[ACGT]ACC
BstPI 2746 GGTAACC G^GT[ACGT]ACC
BstUI 1975 CGCG CG^CG
Cfr10I 2423 GCCGGT [GA]^CCGG[CT]
CpoI 1535 CGGACCG CG^G[AT]CCG
DraI 3456 TTTAAA TTT^AAA
Eco47III 2615 AGCGCT AGC^GCT
EcoO65I 2746 GGTAACC G^GT[ACGT]ACC
EcoRI 3397 GAATTC G^AATTC
EcoT22I 2253 ATGCAT ATGCA^T
FauI 2474 CCCGC CCCGC(4/6)
FspI 1592 TGCGCA TGC^GCA
HaeII 2615 AGCGCT [GA]GCGC^[CT]
MamI 1252 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1975 CGCG CG^CG
NsiI 2253 ATGCAT ATGCA^T
NspI 199 GCATGC [GA]CATG^[CT]
NspV 909 TTCGAA TT^CGAA
PflFI 2125 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2533 ATTAAT AT^TAAT
Psp1406I 925 AACGTT AA^CGTT
PstI 2673 CTGCAG CTGCA^G
PvuI 1725 CGATCG CGAT^CG
RsrII 1535 CGGACCG CG^G[AT]CCG
SapI 2545 GAAGAGC GCTCTTC(1/4)
SexAI 2126 ACCTGGT A^CC[AT]GGT
SfuI 909 TTCGAA TT^CGAA
SphI 199 GCATGC GCATG^C
SspBI 1333 TGTACA T^GTACA
SspI 852 AATATT AAT^ATT
Tth111I 2125 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 2784 CTTAAG C^TTAAG
2890 CTTAAG
AhdI 295 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3187 GACGTCTGGTC
AlwNI 1585 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2582 CAGCAACTG
Asp700I 1476 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2896 GAATATCTTC
AspEI 295 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3187 GACGTCTGGTC
BamHI 1730 GGATCC G^GATCC
2630 GGATCC
BbsI 69 GAAGAC GAAGAC(2/6)
2836 GAAGAC
BfrI 2784 CTTAAG C^TTAAG
2890 CTTAAG
BpuAI 69 GAAGAC GAAGAC(2/6)
2836 GAAGAC
BsaWI 320 ACCGGA [AT]^CCGG[AT]
2039 ACCGGA
BssSI 1195 CACGAG CACGAG(-5/-1)
1776 CACGAG
BstAPI 1584 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3213 GCATGAGCTGC
CfoI 1593 GCGC GCG^C
2616 GCGC
DraIII 1945 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
3043 CACCAAGTG
Eam1105I 295 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3187 GACGTCTGGTC
EcoNI 94 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1734 CCTCAAGAAGG
HhaI 1593 GCGC GCG^C
2616 GCGC
HinP1I 1593 GCGC G^CGC
2616 GCGC
HindIII 2539 AAGCTT A^AGCTT
3420 AAGCTT
MfeI 342 CAATTG C^AATTG
3080 CAATTG
MunI 342 CAATTG C^AATTG
3080 CAATTG
PpuMI 1798 GGGACCC [GA]G^G[AT]CC[CT]
2016 GGGTCCT
PvuII 1714 CAGCTG CAG^CTG
2089 CAGCTG
SacI 60 GAGCTC GAGCT^C
2668 GAGCTC
SfcI 1518 CTACAG C^T[GA][CT]AG
2673 CTGCAG
StuI 1202 AGGCCT AGG^CCT
2503 AGGCCT
XcmI 1655 CCAACCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3045 CCAAGTGAAAATTGG
XmnI 1476 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2896 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII