Restriction map for
pF1KB3481
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BcgI
BglI BlnI BsaAI BsaBI BsiEI
BsiWI BspDI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI PvuI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 890 TCCGGA T^CCGGA
AclI 845 AACGTT AA^CGTT
AflII 594 CTTAAG C^TTAAG
AflIII 2313 ACATGT A^C[GA][CT]GT
Aor51HI 2333 AGCGCT AGC^GCT
ApaI 685 GGGCCC GGGCC^C
Asp700I 106 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 798 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 243 TGGCCA TGG^CCA
BbeI 2301 GGCGCC GGCGC^C
BbsI 2095 GAAGAC GAAGAC(2/6)
BbvCI 1522 GCTGAGG CCTCAGC(-5/-2)
BciVI 1987 GTATCC GTATCC(6/5)
BfaI 1918 CTAG C^TAG
BfrI 594 CTTAAG C^TTAAG
BlpI 426 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 426 GCTCAGC GC^T[ACGT]AGC
BpuAI 2095 GAAGAC GAAGAC(2/6)
BsaI 11 GAGACC GGTCTC(1/5)
BsaWI 890 TCCGGA [AT]^CCGG[AT]
BseAI 890 TCCGGA T^CCGGA
BsgI 1372 GTGCAG GTGCAG(16/14)
BsmBI 1751 CGTCTC CGTCTC(1/5)
BsmI 586 GAATGC GAATGC(1/-1)
BspEI 890 TCCGGA T^CCGGA
BspLU11I 2313 ACATGT A^CATGT
BspMI 132 GCAGGT ACCTGC(4/8)
BsrFI 1169 GCCGGT [GA]^CCGG[CT]
CelII 426 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1169 GCCGGT [GA]^CCGG[CT]
CpoI 20 CGGACCG CG^G[AT]CCG
DraI 1437 TTTAAA TTT^AAA
EaeI 243 TGGCCA [CT]^GGCC[GA]
Eco47III 2333 AGCGCT AGC^GCT
EcoRV 913 GATATC GAT^ATC
HincII 904 GTCAAC GT[CT]^[GA]AC
HindII 904 GTCAAC GT[CT]^[GA]AC
KasI 2301 GGCGCC G^GCGCC
MaeI 1918 CTAG C^TAG
MluNI 243 TGGCCA TGG^CCA
MroI 890 TCCGGA T^CCGGA
MscI 243 TGGCCA TGG^CCA
NarI 2301 GGCGCC GG^CGCC
PflFI 798 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 845 AACGTT AA^CGTT
PstI 1486 CTGCAG CTGCA^G
RsrII 20 CGGACCG CG^G[AT]CCG
SacI 622 GAGCTC GAGCT^C
SfoI 2301 GGCGCC GGC^GCC
SmaI 87 CCCGGG CCC^GGG
SphI 1060 GCATGC GCATG^C
SwaI 1436 ATTTAAAT ATTT^AAAT
Tth111I 798 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
XmnI 106 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 995 GTGCAC G^TGCAC
1327 GTGCAC
AlwNI 1633 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2153 CAGCCACTG
AocI 100 CCTCAGG CC^T[ACGT]AGG
417 CCTCAGG
ApaLI 995 GTGCAC G^TGCAC
1327 GTGCAC
AvaI 87 CCCGGG C^[CT]CG[GA]G
698 CCCGAG
BanII 622 GAGCTC G[GA]GC[CT]^C
685 GGGCCC
BclI 1640 TGATCA T^GATCA
2066 TGATCA
BglII 1163 AGATCT A^GATCT
1874 AGATCT
BsmFI 442 GTCCC GGGAC(10/14)
1038 GTCCC
BsoBI 87 CCCGGG C^[CT]CG[GA]G
698 CCCGAG
BspHI 1310 TCATGA T^CATGA
1650 TCATGA
BsrBI 2323 CCGCTC CCGCTC(-3/-3)
17 GAGCGG
BstXI 1472 CCAACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2156 CCACTGACCTGG
Bsu36I 100 CCTCAGG CC^T[ACGT]AGG
417 CCTCAGG
DsaI 734 CCATGG C^C[GA][CT]GG
1884 CCATGG
Eco81I 100 CCTCAGG CC^T[ACGT]AGG
417 CCTCAGG
FbaI 1640 TGATCA T^GATCA
2066 TGATCA
HgaI 82 GACGC GACGC(5/10)
1899 GCGTC
NcoI 734 CCATGG C^CATGG
1884 CCATGG
NspI 1060 GCATGC [GA]CATG^[CT]
2313 ACATGT
PpuMI 569 AGGACCC [GA]G^G[AT]CC[CT]
1255 AGGTCCT
RcaI 1310 TCATGA T^CATGA
1650 TCATGA
SfcI 180 CTACAG C^T[GA][CT]AG
1486 CTGCAG
SmlI 594 CTTAAG C^T[CT][GA]AG
1288 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PshAI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII