Restriction map for
pF1KB3465
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 395 CGCG CG^CG
Alw44I 888 GTGCAC G^TGCAC
AlwNI 17 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 888 GTGCAC G^TGCAC
AspHI 888 GTGCAC G[AT]GC[AT]^C
AspI 43 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 414 CCCGGG C^[CT]CG[GA]G
BamHI 419 GGATCC G^GATCC
BanII 411 GAGCCC G[GA]GC[CT]^C
BfaI 1030 CTAG C^TAG
BsaBI 625 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 263 ACCGGA [AT]^CCGG[AT]
BsiHKAI 888 GTGCAC G[AT]GC[AT]^C
BsoBI 414 CCCGGG C^[CT]CG[GA]G
BsrDI 122 CATTGC GCAATG(2/0)
BsrFI 438 GCCGGT [GA]^CCGG[CT]
BstUI 395 CGCG CG^CG
BstYI 419 GGATCC [GA]^GATC[CT]
Cfr10I 438 GCCGGT [GA]^CCGG[CT]
DsaI 38 CCATGG C^C[GA][CT]GG
EarI 726 GAAGAG CTCTTC(1/4)
EcoNI 328 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 228 GAATTC G^AATTC
EcoT14I 38 CCATGG C^C[AT][AT]GG
FauI 675 CCCGC CCCGC(4/6)
HaeIII 36 GGCC GG^CC
Ksp632I 726 GAAGAG CTCTTC(1/4)
MaeI 1030 CTAG C^TAG
MamI 625 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 419 GGATCC [GA]^GATC[CT]
MslI 778 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 395 CGCG CG^CG
MwoI 360 GCTGTCCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 38 CCATGG C^CATGG
NdeI 560 CATATG CA^TATG
PflFI 43 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 435 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 383 CAGCTG CAG^CTG
SfaNI 797 GATGC GCATC(5/9)
SmaI 414 CCCGGG CCC^GGG
StyI 38 CCATGG C^C[AT][AT]GG
TaqI 608 TCGA T^CGA
Tth111I 43 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 608 TCGA T^CGA
Van91I 435 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 419 GGATCC [GA]^GATC[CT]
XmaCI 414 CCCGGG C^CCGGG
XmaI 414 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 228 GAATTC [GA]^AATT[CT]
522 AAATTC
ApoI 228 GAATTC [GA]^AATT[CT]
522 AAATTC
AvaII 42 GGACC G^G[AT]CC
186 GGACC
BciVI 753 GGATAC GTATCC(6/5)
773 GGATAC
BpmI 332 CTCCAG CTGGAG(16/14)
481 CTCCAG
BsaI 729 GAGACC GGTCTC(1/5)
498 GGTCTC
BseRI 237 CTCCTC GAGGAG(10/8)
511 GAGGAG
BsmFI 457 GTCCC GGGAC(10/14)
669 GTCCC
BstF5I 432 CATCC GGATG(2/0)
796 GGATG
Cfr13I 42 GGACC G^G[ACGT]CC
186 GGACC
DpnII 420 GATC ^GATC
606 GATC
FokI 432 CATCC GGATG(9/13)
796 GGATG
MboI 420 GATC ^GATC
606 GATC
MseI 590 TTAA T^TAA
655 TTAA
MspA1I 358 CCGCTG C[AC]G^C[GT]G
383 CAGCTG
NdeII 420 GATC ^GATC
606 GATC
Sau3AI 420 GATC ^GATC
606 GATC
Sau96I 42 GGACC G^G[ACGT]CC
186 GGACC
SphI 287 GCATGC GCATG^C
536 GCATGC
Tru9I 590 TTAA T^TAA
655 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstNI Cac8I DdeI
EcoRII Fnu4HI HapII HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspI MvaI NciI NlaIII NlaIV
NspI RsaI ScrFI SspBI TfiI
TseI Tsp45I Tsp509I TspRI