Restriction map for
pF1KB3457
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MslI MspA1I
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 490 GCGG CCGC(-3/-1)
AcsI 133 GAATTC [GA]^AATT[CT]
AlwI 493 GGATC GGATC(4/5)
ApoI 133 GAATTC [GA]^AATT[CT]
AvaI 104 CTCGAG C^[CT]CG[GA]G
BalI 92 TGGCCA TGG^CCA
BbsI 189 GAAGAC GAAGAC(2/6)
BbvCI 467 CCTCAGC CCTCAGC(-5/-2)
BclI 62 TGATCA T^GATCA
BcnI 87 CCGGG CC^[GC]GG
BpuAI 189 GAAGAC GAAGAC(2/6)
BsaBI 22 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 330 CCTTGG C^C[ACGT][ACGT]GG
BsgI 126 CTGCAC GTGCAG(16/14)
BsmI 335 GCATTC GAATGC(1/-1)
BsoBI 104 CTCGAG C^[CT]CG[GA]G
BssKI 87 CCGGG ^CC[ACGT]GG
BstF5I 504 GGATG GGATG(2/0)
BstYI 493 GGATCT [GA]^GATC[CT]
Cfr13I 408 GGCCC G^G[ACGT]CC
EaeI 92 TGGCCA [CT]^GGCC[GA]
EcoRI 133 GAATTC G^AATTC
EcoRV 523 GATATC GAT^ATC
EcoT14I 330 CCTTGG C^C[AT][AT]GG
FauI 490 GCGGG CCCGC(4/6)
FbaI 62 TGATCA T^GATCA
FokI 504 GGATG GGATG(9/13)
HapII 87 CCGG C^CGG
HinfI 72 GACTC G^A[ACGT]TC
HpaII 87 CCGG C^CGG
MaeIII 173 GTAAC ^GT[ACGT]AC
MamI 22 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 493 GGATCT [GA]^GATC[CT]
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
MseI 295 TTAA T^TAA
MspI 87 CCGG C^CGG
NciI 87 CCGGG CC^[GC]GG
NlaIII 208 CATG CATG^
NspI 207 GCATGT [GA]CATG^[CT]
PaeR7I 104 CTCGAG C^TCGAG
PleI 72 GACTC GAGTC(4/5)
Sau96I 408 GGCCC G^G[ACGT]CC
ScrFI 87 CCGGG CC^[ACGT]GG
SmlI 104 CTCGAG C^T[CT][GA]AG
StyI 330 CCTTGG C^C[AT][AT]GG
TaqI 105 TCGA T^CGA
Tru9I 295 TTAA T^TAA
TthHB8I 105 TCGA T^CGA
XcmI 311 CCAGTTCTCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 104 CTCGAG C^TCGAG
XhoII 493 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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Asp700I 133 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
418 GAATGGCTTC
BfaI 223 CTAG C^TAG
386 CTAG
BsmAI 70 GAGAC GTCTC(1/5)
357 GAGAC
BsrI 311 CCAGT ACTGG(1/-1)
473 CCAGT
Cac8I 435 GCAAGC GC[ACGT]^[ACGT]GC
456 GCTTGC
DdeI 468 CTCAG C^T[ACGT]AG
500 CTCAG
EarI 74 CTCTTC CTCTTC(1/4)
346 GAAGAG
Ksp632I 74 CTCTTC CTCTTC(1/4)
346 GAAGAG
MaeI 223 CTAG C^TAG
386 CTAG
MnlI 8 CCTC CCTC(7/6)
467 CCTC
SfaNI 100 GATGC GCATC(5/9)
478 GCATC
XmnI 133 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
418 GAATGGCTTC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AluI BbvI DpnII Fnu4HI HaeIII
HphI ItaI MboI MboII MwoI
NdeII Sau3AI TseI Tsp509I TspRI