Restriction map for
pF1KB3414
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DrdI
Eco47III EcoNI EcoO65I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI Psp1406I RcaI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI SwaI Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1142 GGTACC G^GTACC
AflIII 1095 ACATGT A^C[GA][CT]GT
AocI 1569 CCTGAGG CC^T[ACGT]AGG
AseI 1245 ATTAAT AT^TAAT
AsnI 1245 ATTAAT AT^TAAT
Asp718I 1142 GGTACC G^GTACC
AvaI 19 CTCGAG C^[CT]CG[GA]G
BanI 1142 GGTACC G^G[CT][GA]CC
BanII 1192 GAGCCC G[GA]GC[CT]^C
BbvCI 391 CCTCAGC CCTCAGC(-5/-2)
BglI 1882 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1805 AGATCT A^GATCT
BsaI 1375 GGTCTC GGTCTC(1/5)
BsgI 353 GTGCAG GTGCAG(16/14)
BsmI 585 GAATGC GAATGC(1/-1)
BsoBI 19 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2476 TGTACA T^GTACA
BspLU11I 1095 ACATGT A^CATGT
BsrFI 311 ACCGGC [GA]^CCGG[CT]
BsrGI 2476 TGTACA T^GTACA
BstAPI 1453 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1569 CCTGAGG CC^T[ACGT]AGG
CfoI 61 GCGC GCG^C
Cfr10I 311 ACCGGC [GA]^CCGG[CT]
DraII 768 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 86 CCGTGG C^C[GA][CT]GG
EagI 83 CGGCCG C^GGCCG
EclXI 83 CGGCCG C^GGCCG
Eco52I 83 CGGCCG C^GGCCG
Eco81I 1569 CCTGAGG CC^T[ACGT]AGG
EcoO109I 768 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 211 GATATC GAT^ATC
EcoT14I 1619 CCAAGG C^C[AT][AT]GG
HhaI 61 GCGC GCG^C
HinP1I 61 GCGC G^CGC
HindIII 887 AAGCTT A^AGCTT
HpaI 2538 GTTAAC GTT^AAC
KpnI 1142 GGTACC GGTAC^C
NdeI 1583 CATATG CA^TATG
NheI 573 GCTAGC G^CTAGC
NspI 1095 ACATGT [GA]CATG^[CT]
PaeR7I 19 CTCGAG C^TCGAG
PpuMI 768 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1650 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1245 ATTAAT AT^TAAT
PstI 1451 CTGCAG CTGCA^G
PvuI 39 CGATCG CGAT^CG
SalI 444 GTCGAC G^TCGAC
SapI 550 GAAGAGC GCTCTTC(1/4)
SexAI 1991 ACCAGGT A^CC[AT]GGT
SspBI 2476 TGTACA T^GTACA
StyI 1619 CCAAGG C^C[AT][AT]GG
XhoI 19 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 263 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1735 GACCTTCAGTC
Asp700I 382 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2383 GAATTTATTC
AspEI 263 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1735 GACCTTCAGTC
AspI 27 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1084 GACATTGTC
BbsI 24 GAAGAC GAAGAC(2/6)
2559 GAAGAC
BclI 8 TGATCA T^GATCA
1467 TGATCA
BcnI 1146 CCGGG CC^[GC]GG
2352 CCCGG
BpuAI 24 GAAGAC GAAGAC(2/6)
2559 GAAGAC
BsaBI 1624 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2419 GATGAGGATC
BseRI 1572 GAGGAG GAGGAG(10/8)
2174 GAGGAG
BsiEI 39 CGATCG CG[GA][CT]^CG
83 CGGCCG
BsmFI 2468 GGGAC GGGAC(10/14)
1090 GTCCC
BsrDI 2409 CATTGC GCAATG(2/0)
799 GCAATG
BstXI 122 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2406 CCACATTGCTGG
DraI 1948 TTTAAA TTT^AAA
2182 TTTAAA
DraIII 329 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
675 CACACAGTG
Eam1105I 263 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1735 GACCTTCAGTC
EcoRI 841 GAATTC G^AATTC
1924 GAATTC
FbaI 8 TGATCA T^GATCA
1467 TGATCA
HincII 444 GTCGAC GT[CT]^[GA]AC
2538 GTTAAC
HindII 444 GTCGAC GT[CT]^[GA]AC
2538 GTTAAC
MamI 1624 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2419 GATGAGGATC
NciI 1146 CCGGG CC^[GC]GG
2352 CCCGG
PflFI 27 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1084 GACATTGTC
SmlI 19 CTCGAG C^T[CT][GA]AG
1930 CTCAAG
SpeI 1212 ACTAGT A^CTAGT
1902 ACTAGT
StuI 1943 AGGCCT AGG^CCT
2222 AGGCCT
Tth111I 27 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1084 GACATTGTC
XmnI 382 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2383 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII