Restriction map for
pF1KB3410
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII BbeI
BbvCI BciVI BclI BfrI BglII
BsaBI BsaWI BseAI BsiEI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 403 GACGCC G[GA]^CG[CT]C
AhdI 403 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1084 GTGCAC G^TGCAC
ApaI 796 GGGCCC GGGCC^C
ApaLI 1084 GTGCAC G^TGCAC
AspEI 403 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 802 CCCGAG C^[CT]CG[GA]G
AvrII 1689 CCTAGG C^CTAGG
BalI 2186 TGGCCA TGG^CCA
BbrPI 2194 CACGTG CAC^GTG
BcgI 1784 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1690 CTAG C^TAG
BglI 559 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1689 CCTAGG C^CTAGG
BsaHI 403 GACGCC G[GA]^CG[CT]C
BsaI 2173 GAGACC GGTCTC(1/5)
BsiWI 1053 CGTACG C^GTACG
BsmI 2028 GAATGC GAATGC(1/-1)
BsoBI 802 CCCGAG C^[CT]CG[GA]G
Bsp1407I 338 TGTACA T^GTACA
BsrDI 1355 GCAATG GCAATG(2/0)
BsrGI 338 TGTACA T^GTACA
BssSI 1495 CTCGTG CACGAG(-5/-1)
BstEII 981 GGTGACC G^GT[ACGT]ACC
BstPI 981 GGTGACC G^GT[ACGT]ACC
DraIII 220 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 403 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1263 GAAGAG CTCTTC(1/4)
EcoO65I 981 GGTGACC G^GT[ACGT]ACC
EcoRI 2158 GAATTC G^AATTC
EcoT22I 2030 ATGCAT ATGCA^T
Hin1I 403 GACGCC G[GA]^CG[CT]C
Ksp632I 1263 GAAGAG CTCTTC(1/4)
MaeI 1690 CTAG C^TAG
MfeI 372 CAATTG C^AATTG
MluNI 2186 TGGCCA TGG^CCA
MscI 2186 TGGCCA TGG^CCA
MseI 1148 TTAA T^TAA
MunI 372 CAATTG C^AATTG
NdeI 290 CATATG CA^TATG
NsiI 2030 ATGCAT ATGCA^T
PmaCI 2194 CACGTG CAC^GTG
PmlI 2194 CACGTG CAC^GTG
SexAI 221 ACCAGGT A^CC[AT]GGT
SspBI 338 TGTACA T^GTACA
StuI 635 AGGCCT AGG^CCT
TfiI 1092 GAATC G^A[AT]TC
Tru9I 1148 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 1447 AAATTC [GA]^AATT[CT]
2158 GAATTC
AflIII 1253 ACATGT A^C[GA][CT]GT
2114 ACATGT
AocI 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
ApoI 1447 AAATTC [GA]^AATT[CT]
2158 GAATTC
Asp700I 1512 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1836 GAAACCATTC
BanI 1228 GGCACC G^G[CT][GA]CC
1386 GGTGCC
BlpI 573 GCTGAGC GC^T[ACGT]AGC
886 GCTGAGC
Bpu1102I 573 GCTGAGC GC^T[ACGT]AGC
886 GCTGAGC
BsaAI 2061 CACGTA [CT]AC^GT[GA]
2194 CACGTG
BsmBI 35 GAGACG CGTCTC(1/5)
601 GAGACG
BspLU11I 1253 ACATGT A^CATGT
2114 ACATGT
Bsu36I 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
CelII 573 GCTGAGC GC^T[ACGT]AGC
886 GCTGAGC
Eco81I 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
EcoNI 189 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1479 CCTGACCAAGG
HaeII 1434 GGCGCT [GA]GCGC^[CT]
2056 AGCGCC
HincII 400 GTTGAC GT[CT]^[GA]AC
946 GTCAAC
HindII 400 GTTGAC GT[CT]^[GA]AC
946 GTCAAC
NcoI 124 CCATGG C^CATGG
2177 CCATGG
NspI 1253 ACATGT [GA]CATG^[CT]
2114 ACATGT
PflMI 174 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1790 CCATCGCCTGG
ScaI 509 AGTACT AGT^ACT
1949 AGTACT
Van91I 174 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1790 CCATCGCCTGG
XmnI 1512 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1836 GAAACCATTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BamHI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII