Restriction map for
pF1KB3348
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbsI
BbvCI BclI BfrI BglII BlnI
BpuAI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
BssSI BstBI BstEII BstPI Bsu36I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SphI SspI SwaI
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1414 GACGTC GACGT^C
AccI 1279 GTATAC GT^[AC][GT]AC
AcyI 1414 GACGTC G[GA]^CG[CT]C
AseI 501 ATTAAT AT^TAAT
AsnI 501 ATTAAT AT^TAAT
AvaI 1853 CCCGGG C^[CT]CG[GA]G
BamHI 671 GGATCC G^GATCC
BbrPI 260 CACGTG CAC^GTG
BciVI 765 GGATAC GTATCC(6/5)
BglI 626 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 260 CACGTG [CT]AC^GT[GA]
BsaBI 528 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1414 GACGTC G[GA]^CG[CT]C
BsaWI 1669 TCCGGT [AT]^CCGG[AT]
BsmBI 1416 CGTCTC CGTCTC(1/5)
BsoBI 1853 CCCGGG C^[CT]CG[GA]G
Bsp1407I 926 TGTACA T^GTACA
BsrBI 202 CCGCTC CCGCTC(-3/-3)
BsrGI 926 TGTACA T^GTACA
BssHII 2001 GCGCGC G^CGCGC
Bst1107I 1279 GTATAC GTA^TAC
BstXI 106 CCAGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1279 GTATAC GTA^TAC
DraI 795 TTTAAA TTT^AAA
DrdI 1870 GACCCGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1887 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1944 GAATTC G^AATTC
EcoT14I 292 CCTTGG C^C[AT][AT]GG
HaeII 1497 GGCGCT [GA]GCGC^[CT]
HgaI 46 GACGC GACGC(5/10)
Hin1I 1414 GACGTC G[GA]^CG[CT]C
HindIII 2044 AAGCTT A^AGCTT
KspI 1873 CCGCGG CCGC^GG
MamI 528 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 896 ACATGC [GA]CATG^[CT]
PmaCI 260 CACGTG CAC^GTG
PmlI 260 CACGTG CAC^GTG
PshBI 501 ATTAAT AT^TAAT
SacII 1873 CCGCGG CCGC^GG
SapI 1524 GAAGAGC GCTCTTC(1/4)
SbfI 1932 CCTGCAGG CCTGCA^GG
SexAI 389 ACCAGGT A^CC[AT]GGT
SmaI 1853 CCCGGG CCC^GGG
SpeI 1900 ACTAGT A^CTAGT
Sse8387I 1932 CCTGCAGG CCTGCA^GG
SspBI 926 TGTACA T^GTACA
StuI 1447 AGGCCT AGG^CCT
StyI 292 CCTTGG C^C[AT][AT]GG
XmaCI 1853 CCCGGG C^CCGGG
XmaI 1853 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1405 AAATTC [GA]^AATT[CT]
1944 GAATTC
ApaI 72 GGGCCC GGGCC^C
1856 GGGCCC
ApoI 1405 AAATTC [GA]^AATT[CT]
1944 GAATTC
Asp700I 946 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGGTTTTC
BcgI 628 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1017 GCAGACCGTTCG
BfaI 1901 CTAG C^TAG
1909 CTAG
DsaI 1873 CCGCGG C^C[GA][CT]GG
1950 CCGTGG
EarI 592 CTCTTC CTCTTC(1/4)
1524 GAAGAG
Ksp632I 592 CTCTTC CTCTTC(1/4)
1524 GAAGAG
MaeI 1901 CTAG C^TAG
1909 CTAG
MseI 502 TTAA T^TAA
796 TTAA
MslI 771 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1750 CACGCAGATG
PflMI 1767 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2010 CCAGATCCTGG
PleI 872 GACTC GAGTC(4/5)
278 GAGTC
SacI 759 GAGCTC GAGCT^C
1207 GAGCTC
SmlI 95 CTCAAG C^T[CT][GA]AG
1456 CTCAAG
TaqI 657 TCGA T^CGA
870 TCGA
TfiI 659 GAATC G^A[AT]TC
1551 GAATC
Tru9I 502 TTAA T^TAA
796 TTAA
Tsp45I 339 GTCAC ^GT[GC]AC
1492 GTGAC
Tsp509I 1406 AATT ^AATT
1945 AATT
TthHB8I 657 TCGA T^CGA
870 TCGA
Van91I 1767 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2010 CCAGATCCTGG
XmnI 946 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI TspRI XhoII