Restriction map for
pF1KB3319
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1879 GTAGAC GT^[AC][GT]AC
Alw44I 175 GTGCAC G^TGCAC
AocI 2290 CCTGAGG CC^T[ACGT]AGG
ApaLI 175 GTGCAC G^TGCAC
Asp700I 624 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 293 CCCGAG C^[CT]CG[GA]G
AviII 2006 TGCGCA TGC^GCA
BbeI 1888 GGCGCC GGCGC^C
BbsI 519 GAAGAC GAAGAC(2/6)
BbvCI 2176 GCTGAGG CCTCAGC(-5/-2)
BcgI 1039 GCATCCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2256 GGATAC GTATCC(6/5)
BpuAI 519 GAAGAC GAAGAC(2/6)
BsoBI 293 CCCGAG C^[CT]CG[GA]G
BsrBI 1491 CCGCTC CCGCTC(-3/-3)
BstAPI 2314 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2290 CCTGAGG CC^T[ACGT]AGG
Eco81I 2290 CCTGAGG CC^T[ACGT]AGG
FspI 2006 TGCGCA TGC^GCA
KasI 1888 GGCGCC G^GCGCC
MseI 314 TTAA T^TAA
NarI 1888 GGCGCC GG^CGCC
NcoI 1001 CCATGG C^CATGG
NheI 859 GCTAGC G^CTAGC
NspI 1076 GCATGC [GA]CATG^[CT]
SapI 880 GAAGAGC GCTCTTC(1/4)
SfiI 2404 GGCCCCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1888 GGCGCC GGC^GCC
SgrAI 1333 CACCGGCG C[GA]^CCGG[CT]G
SmlI 318 CTTGAG C^T[CT][GA]AG
SphI 1076 GCATGC GCATG^C
Tru9I 314 TTAA T^TAA
Tsp509I 2234 AATT ^AATT
XcmI 676 CCACCTAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 624 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 1270 GACGCC G[GA]^CG[CT]C
1888 GGCGCC
AspI 226 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACCGCGTC
BamHI 1149 GGATCC G^GATCC
2166 GGATCC
BglI 1744 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2405 GCCCCCTTGGC
BglII 257 AGATCT A^GATCT
356 AGATCT
BsaHI 1270 GACGCC G[GA]^CG[CT]C
1888 GGCGCC
Hin1I 1270 GACGCC G[GA]^CG[CT]C
1888 GGCGCC
NaeI 1744 GCCGGC GCC^GGC
2110 GCCGGC
NgoMIV 1744 GCCGGC G^CCGGC
2110 GCCGGC
PflFI 226 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACCGCGTC
PstI 1597 CTGCAG CTGCA^G
2115 CTGCAG
SacI 595 GAGCTC GAGCT^C
1981 GAGCTC
SfcI 1597 CTGCAG C^T[GA][CT]AG
2115 CTGCAG
TaqI 1048 TCGA T^CGA
1707 TCGA
Tth111I 226 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACCGCGTC
TthHB8I 1048 TCGA T^CGA
1707 TCGA
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp45I TspRI Van91I
XhoII