Restriction map for
pF1KB3311
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbvCI
BfaI BfrI BlnI BsaBI BsaI
BsaWI BseAI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MaeIII MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI SbfI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1016 GACGTC GACGT^C
AccI 1006 GTAGAC GT^[AC][GT]AC
AcsI 1566 AAATTT [GA]^AATT[CT]
AflIII 871 ACGTGT A^C[GA][CT]GT
AhdI 1822 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1566 AAATTT [GA]^AATT[CT]
Asp700I 1357 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1822 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 849 GAGCTC G[AT]GC[AT]^C
AvaI 195 CTCGAG C^[CT]CG[GA]G
BalI 740 TGGCCA TGG^CCA
BbrPI 870 CACGTG CAC^GTG
BbsI 1819 GAAGAC GAAGAC(2/6)
BciVI 1339 GTATCC GTATCC(6/5)
BpuAI 1819 GAAGAC GAAGAC(2/6)
BsaAI 870 CACGTG [CT]AC^GT[GA]
BsiHKAI 849 GAGCTC G[AT]GC[AT]^C
BsiWI 725 CGTACG C^GTACG
BsmBI 1014 GAGACG CGTCTC(1/5)
BsmI 1197 GAATGC GAATGC(1/-1)
BsoBI 195 CTCGAG C^[CT]CG[GA]G
BspHI 89 TCATGA T^CATGA
BspMI 922 ACCTGC ACCTGC(4/8)
BsrBI 565 CCGCTC CCGCTC(-3/-3)
BsrDI 1487 GCAATG GCAATG(2/0)
DraI 1695 TTTAAA TTT^AAA
DraII 1745 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1822 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1745 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1588 GATATC GAT^ATC
KspI 689 CCGCGG CCGC^GG
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
MslI 390 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 116 CCATGG C^CATGG
NspI 1296 GCATGT [GA]CATG^[CT]
PaeR7I 195 CTCGAG C^TCGAG
PflMI 1343 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 870 CACGTG CAC^GTG
PmlI 870 CACGTG CAC^GTG
PpuMI 1745 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1277 CTGCAG CTGCA^G
RcaI 89 TCATGA T^CATGA
SacI 849 GAGCTC GAGCT^C
SacII 689 CCGCGG CCGC^GG
ScaI 1034 AGTACT AGT^ACT
SfiI 753 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I 1343 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 107 CCAAGCTGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 195 CTCGAG C^TCGAG
XmnI 1357 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BbeI 270 GGCGCC GGCGC^C
486 GGCGCC
BcgI 1507 CGACTTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
475 GCACTGCCCTCG
BclI 248 TGATCA T^GATCA
1625 TGATCA
BglII 1236 AGATCT A^GATCT
1775 AGATCT
BlpI 660 GCTCAGC GC^T[ACGT]AGC
1291 GCTGAGC
Bpu1102I 660 GCTCAGC GC^T[ACGT]AGC
1291 GCTGAGC
BsgI 1779 CTGCAC GTGCAG(16/14)
958 GTGCAG
BsiEI 614 CGGCCG CG[GA][CT]^CG
647 CGGCCG
BsrI 44 CCAGT ACTGG(1/-1)
1003 CCAGT
BssHII 554 GCGCGC G^CGCGC
556 GCGCGC
BstF5I 344 CATCC GGATG(2/0)
595 CATCC
BstXI 803 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1742 CCAAGGTCCTGG
BstYI 1236 AGATCT [GA]^GATC[CT]
1775 AGATCT
CelII 660 GCTCAGC GC^T[ACGT]AGC
1291 GCTGAGC
EagI 614 CGGCCG C^GGCCG
647 CGGCCG
EclXI 614 CGGCCG C^GGCCG
647 CGGCCG
Eco52I 614 CGGCCG C^GGCCG
647 CGGCCG
FbaI 248 TGATCA T^GATCA
1625 TGATCA
FokI 344 CATCC GGATG(9/13)
595 CATCC
HaeII 270 GGCGCC [GA]GCGC^[CT]
486 GGCGCC
HgaI 8 GCGTC GACGC(5/10)
799 GCGTC
KasI 270 GGCGCC G^GCGCC
486 GGCGCC
MflI 1236 AGATCT [GA]^GATC[CT]
1775 AGATCT
NarI 270 GGCGCC GG^CGCC
486 GGCGCC
SfcI 146 CTACAG C^T[GA][CT]AG
1277 CTGCAG
SfoI 270 GGCGCC GGC^GCC
486 GGCGCC
TfiI 1362 GATTC G^A[AT]TC
1411 GAATC
Tsp509I 1307 AATT ^AATT
1567 AATT
XhoII 1236 AGATCT [GA]^GATC[CT]
1775 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI TspRI TthHB8I