Restriction map for
pF1KB3305
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 45 CGCG CG^CG
AflIII 1476 ACGTGT A^C[GA][CT]GT
Alw44I 736 GTGCAC G^TGCAC
AlwNI 1490 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 748 CCTGAGG CC^T[ACGT]AGG
ApaI 557 GGGCCC GGGCC^C
ApaLI 736 GTGCAC G^TGCAC
BalI 420 TGGCCA TGG^CCA
BbvCI 1555 GCTGAGG CCTCAGC(-5/-2)
BglI 500 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 77 AGATCT A^GATCT
BpmI 1462 CTGGAG CTGGAG(16/14)
BsaAI 1475 TACGTG [CT]AC^GT[GA]
BspHI 407 TCATGA T^CATGA
BstUI 45 CGCG CG^CG
BstXI 1634 CCAGTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 748 CCTGAGG CC^T[ACGT]AGG
DsaI 44 CCGCGG C^C[GA][CT]GG
Eco81I 748 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1445 CCAAGG C^C[AT][AT]GG
EcoT22I 790 ATGCAT ATGCA^T
HgaI 867 GCGTC GACGC(5/10)
HindIII 7 AAGCTT A^AGCTT
KspI 44 CCGCGG CCGC^GG
MluNI 420 TGGCCA TGG^CCA
MscI 420 TGGCCA TGG^CCA
MseI 1679 TTAA T^TAA
MvnI 45 CGCG CG^CG
NsiI 790 ATGCAT ATGCA^T
PleI 1342 GACTC GAGTC(4/5)
PpuMI 483 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1078 CTGCAG CTGCA^G
RcaI 407 TCATGA T^CATGA
SacII 44 CCGCGG CCGC^GG
ScaI 285 AGTACT AGT^ACT
SexAI 1598 ACCAGGT A^CC[AT]GGT
SmlI 1420 CTCAAG C^T[CT][GA]AG
SspI 259 AATATT AAT^ATT
StuI 1046 AGGCCT AGG^CCT
StyI 1445 CCAAGG C^C[AT][AT]GG
TfiI 1406 GAATC G^A[AT]TC
Tru9I 1679 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 245 AAATTC [GA]^AATT[CT]
1542 GAATTT
ApoI 245 AAATTC [GA]^AATT[CT]
1542 GAATTT
AspI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACCAGGTC
AvaI 518 CTCGGG C^[CT]CG[GA]G
1207 CCCGAG
BanII 537 GGGCTC G[GA]GC[CT]^C
557 GGGCCC
BbeI 497 GGCGCC GGCGC^C
1252 GGCGCC
BsgI 713 GTGCAG GTGCAG(16/14)
1222 GTGCAG
BsmBI 868 CGTCTC CGTCTC(1/5)
352 GAGACG
BsoBI 518 CTCGGG C^[CT]CG[GA]G
1207 CCCGAG
BspMI 709 GCAGGT ACCTGC(4/8)
1224 GCAGGT
EcoNI 894 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1668 CCTGGCCCAGG
HinfI 1342 GACTC G^A[ACGT]TC
1406 GAATC
KasI 497 GGCGCC G^GCGCC
1252 GGCGCC
MaeII 689 ACGT A^CGT
1476 ACGT
NarI 497 GGCGCC GG^CGCC
1252 GGCGCC
PflFI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACCAGGTC
SfaNI 1486 GCATC GCATC(5/9)
1689 GCATC
SfoI 497 GGCGCC GGC^GCC
1252 GGCGCC
TaqI 161 TCGA T^CGA
998 TCGA
Tsp45I 37 GTGAC ^GT[GC]AC
370 GTCAC
Tth111I 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACCAGGTC
TthHB8I 161 TCGA T^CGA
998 TCGA
XcmI 608 CCACCCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1346 CCAAAGTTCGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbvI BcnI
BmyI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TseI Tsp509I
TspRI Van91I XhoII