Restriction map for
pF1KB3287
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AseI AsnI Asp718I
AspI AvrII BbsI BfrI BlnI
BpuAI BsaI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI Eco47III Eco81I EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflIII 2078 ACATGT A^C[GA][CT]GT
AhdI 559 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 2591 GGCGCGCC GG^CGCGCC
Asp700I 462 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 559 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2112 GAGCAC G[AT]GC[AT]^C
BamHI 455 GGATCC G^GATCC
BbeI 15 GGCGCC GGCGC^C
BbrPI 1177 CACGTG CAC^GTG
BbvCI 1191 CCTCAGC CCTCAGC(-5/-2)
BciVI 2573 GTATCC GTATCC(6/5)
BclI 1889 TGATCA T^GATCA
BlpI 2658 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2658 GCTCAGC GC^T[ACGT]AGC
BsaAI 1177 CACGTG [CT]AC^GT[GA]
BsaBI 1890 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2112 GAGCAC G[AT]GC[AT]^C
BsmBI 9 CGTCTC CGTCTC(1/5)
BspLU11I 2078 ACATGT A^CATGT
BspMI 1049 ACCTGC ACCTGC(4/8)
BsrDI 1319 GCAATG GCAATG(2/0)
BssHII 2592 GCGCGC G^CGCGC
BssSI 43 CTCGTG CACGAG(-5/-1)
BstEII 2535 GGTGACC G^GT[ACGT]ACC
BstPI 2535 GGTGACC G^GT[ACGT]ACC
CelII 2658 GCTCAGC GC^T[ACGT]AGC
DraI 516 TTTAAA TTT^AAA
EagI 2800 CGGCCG C^GGCCG
Eam1105I 559 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2800 CGGCCG C^GGCCG
Eco52I 2800 CGGCCG C^GGCCG
EcoO65I 2535 GGTGACC G^GT[ACGT]ACC
FbaI 1889 TGATCA T^GATCA
KasI 15 GGCGCC G^GCGCC
MaeII 1178 ACGT A^CGT
MamI 1890 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2585 GCCGGC GCC^GGC
NarI 15 GGCGCC GG^CGCC
NcoI 101 CCATGG C^CATGG
NdeI 1585 CATATG CA^TATG
NgoMIV 2585 GCCGGC G^CCGGC
PmaCI 1177 CACGTG CAC^GTG
PmlI 1177 CACGTG CAC^GTG
PshAI 287 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 15 GGCGCC GGC^GCC
SgrAI 2584 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 590 CCCGGG CCC^GGG
SspI 2410 AATATT AAT^ATT
TaqI 191 TCGA T^CGA
TthHB8I 191 TCGA T^CGA
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
XmnI 462 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AviII 1052 TGCGCA TGC^GCA
2546 TGCGCA
BcgI 2255 GCATGCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2797 GCACGGCCGTCG
BglII 1016 AGATCT A^GATCT
1482 AGATCT
BsaWI 968 ACCGGA [AT]^CCGG[AT]
1797 TCCGGT
BspHI 1040 TCATGA T^CATGA
1550 TCATGA
BsrBI 604 CCGCTC CCGCTC(-3/-3)
121 GAGCGG
EcoRI 1090 GAATTC G^AATTC
2142 GAATTC
FspI 1052 TGCGCA TGC^GCA
2546 TGCGCA
HaeII 15 GGCGCC [GA]GCGC^[CT]
2633 AGCGCC
HincII 787 GTCAAC GT[CT]^[GA]AC
1701 GTTGAC
HindII 787 GTCAAC GT[CT]^[GA]AC
1701 GTTGAC
KspI 2641 CCGCGG CCGC^GG
2739 CCGCGG
PpuMI 344 AGGTCCC [GA]G^G[AT]CC[CT]
593 GGGACCC
RcaI 1040 TCATGA T^CATGA
1550 TCATGA
SacII 2641 CCGCGG CCGC^GG
2739 CCGCGG
SapI 2109 GAAGAGC GCTCTTC(1/4)
1644 GCTCTTC
ScaI 1769 AGTACT AGT^ACT
2183 AGTACT
SfaNI 928 GATGC GCATC(5/9)
152 GCATC
SphI 575 GCATGC GCATG^C
2255 GCATGC
StuI 1497 AGGCCT AGG^CCT
2394 AGGCCT
XcmI 185 CCAAGGTCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1955 CCATTGAGTCAGTGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII