Restriction map for
pF1KB3283
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI AspEI AvrII BbvCI BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmBI Bsp1407I BspHI BspLU11I
BsrGI BssHII BssSI BstBI BstEII
BstPI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 908 GGTACC G^GTACC
AccI 559 GTATAC GT^[AC][GT]AC
AccIII 2877 TCCGGA T^CCGGA
AflIII 3164 ACGTGT A^C[GA][CT]GT
Alw44I 1941 GTGCAC G^TGCAC
AocI 1192 CCTGAGG CC^T[ACGT]AGG
ApaLI 1941 GTGCAC G^TGCAC
Asp700I 3093 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 908 GGTACC G^GTACC
AspI 3034 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2471 TGCGCA TGC^GCA
BbrPI 3163 CACGTG CAC^GTG
BcgI 1652 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3163 CACGTG [CT]AC^GT[GA]
BseAI 2877 TCCGGA T^CCGGA
BsiEI 3221 CGGCCG CG[GA][CT]^CG
BspDI 1356 ATCGAT AT^CGAT
BspEI 2877 TCCGGA T^CCGGA
Bst1107I 559 GTATAC GTA^TAC
BstZ17I 559 GTATAC GTA^TAC
Bsu36I 1192 CCTGAGG CC^T[ACGT]AGG
ClaI 1356 ATCGAT AT^CGAT
EagI 3221 CGGCCG C^GGCCG
EclXI 3221 CGGCCG C^GGCCG
Eco52I 3221 CGGCCG C^GGCCG
Eco81I 1192 CCTGAGG CC^T[ACGT]AGG
EcoRV 2053 GATATC GAT^ATC
FspI 2471 TGCGCA TGC^GCA
HincII 262 GTCAAC GT[CT]^[GA]AC
HindII 262 GTCAAC GT[CT]^[GA]AC
KpnI 908 GGTACC GGTAC^C
MroI 2877 TCCGGA T^CCGGA
MseI 691 TTAA T^TAA
PaeR7I 95 CTCGAG C^TCGAG
PflFI 3034 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3163 CACGTG CAC^GTG
PmlI 3163 CACGTG CAC^GTG
SacI 461 GAGCTC GAGCT^C
SapI 795 GCTCTTC GCTCTTC(1/4)
SbfI 3358 CCTGCAGG CCTGCA^GG
ScaI 2882 AGTACT AGT^ACT
SphI 3054 GCATGC GCATG^C
Sse8387I 3358 CCTGCAGG CCTGCA^GG
Tru9I 691 TTAA T^TAA
Tth111I 3034 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 95 CTCGAG C^TCGAG
XmnI 3093 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 384 TGGCCA TGG^CCA
1416 TGGCCA
BamHI 627 GGATCC G^GATCC
2627 GGATCC
BfaI 499 CTAG C^TAG
2857 CTAG
BsaWI 1293 ACCGGA [AT]^CCGG[AT]
2877 TCCGGA
BsrBI 793 CCGCTC CCGCTC(-3/-3)
1717 GAGCGG
BstAPI 1338 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1799 GCAGCCGTTGC
DraIII 3153 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3373 CACCAGGTG
KspI 1931 CCGCGG CCGC^GG
2294 CCGCGG
MaeI 499 CTAG C^TAG
2857 CTAG
MaeII 875 ACGT A^CGT
3164 ACGT
MluNI 384 TGGCCA TGG^CCA
1416 TGGCCA
MscI 384 TGGCCA TGG^CCA
1416 TGGCCA
NaeI 773 GCCGGC GCC^GGC
1643 GCCGGC
NgoMIV 773 GCCGGC G^CCGGC
1643 GCCGGC
NspI 212 ACATGC [GA]CATG^[CT]
3054 GCATGC
SacII 1931 CCGCGG CCGC^GG
2294 CCGCGG
SmlI 95 CTCGAG C^T[CT][GA]AG
391 CTCAAG
XcmI 1997 CCAGCCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3067 CCACTGCCTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI