Restriction map for
pF1KB3281
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BsrBI
BssHII BstBI ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 2032 GTATAC GT^[AC][GT]AC
Alw44I 1084 GTGCAC G^TGCAC
ApaI 796 GGGCCC GGGCC^C
ApaLI 1084 GTGCAC G^TGCAC
AvaI 802 CCCGAG C^[CT]CG[GA]G
BfaI 1966 CTAG C^TAG
BsiEI 1971 CGACCG CG[GA][CT]^CG
BsmBI 601 GAGACG CGTCTC(1/5)
BsoBI 802 CCCGAG C^[CT]CG[GA]G
Bsp1407I 338 TGTACA T^GTACA
BspLU11I 2222 ACATGT A^CATGT
BspMI 621 GCAGGT ACCTGC(4/8)
BsrGI 338 TGTACA T^GTACA
BssSI 1588 CTCGTG CACGAG(-5/-1)
Bst1107I 2032 GTATAC GTA^TAC
BstEII 981 GGTGACC G^GT[ACGT]ACC
BstPI 981 GGTGACC G^GT[ACGT]ACC
BstZ17I 2032 GTATAC GTA^TAC
DraIII 220 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 305 CCATGG C^C[GA][CT]GG
EcoO65I 981 GGTGACC G^GT[ACGT]ACC
FseI 2320 GGCCGGCC GGCCGG^CC
HincII 946 GTCAAC GT[CT]^[GA]AC
HindII 946 GTCAAC GT[CT]^[GA]AC
MaeI 1966 CTAG C^TAG
NaeI 2321 GCCGGC GCC^GGC
NcoI 305 CCATGG C^CATGG
NgoMIV 2321 GCCGGC G^CCGGC
NspI 2222 ACATGT [GA]CATG^[CT]
SacI 2311 GAGCTC GAGCT^C
ScaI 509 AGTACT AGT^ACT
SexAI 221 ACCAGGT A^CC[AT]GGT
SspBI 338 TGTACA T^GTACA
StuI 635 AGGCCT AGG^CCT
TaqI 2270 TCGA T^CGA
TthHB8I 2270 TCGA T^CGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 676 CGCG CG^CG
2297 CGCG
AocI 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
Asp700I 37 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1605 GAAACTGTTC
AspI 1380 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACTCAGTC
BalI 66 TGGCCA TGG^CCA
1510 TGGCCA
BamHI 542 GGATCC G^GATCC
2342 GGATCC
BanI 1228 GGCACC G^G[CT][GA]CC
1479 GGTGCC
BbsI 702 GAAGAC GAAGAC(2/6)
1026 GAAGAC
BciVI 11 GGATAC GTATCC(6/5)
2092 GGATAC
BglII 138 AGATCT A^GATCT
1184 AGATCT
BpuAI 702 GAAGAC GAAGAC(2/6)
1026 GAAGAC
BsgI 193 GTGCAG GTGCAG(16/14)
1555 GTGCAG
BsrFI 866 ACCGGC [GA]^CCGG[CT]
2321 GCCGGC
BstAPI 195 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
711 GCAGCTGCTGC
BstUI 676 CGCG CG^CG
2297 CGCG
Bsu36I 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
Cfr10I 866 ACCGGC [GA]^CCGG[CT]
2321 GCCGGC
Eco81I 537 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
EcoNI 189 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1572 CCTGACCAAGG
HaeII 1527 GGCGCT [GA]GCGC^[CT]
2164 AGCGCC
HgaI 588 GCGTC GACGC(5/10)
1202 GCGTC
MluNI 66 TGGCCA TGG^CCA
1510 TGGCCA
MscI 66 TGGCCA TGG^CCA
1510 TGGCCA
MvnI 676 CGCG CG^CG
2297 CGCG
PflFI 1380 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACTCAGTC
PflMI 174 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCATTGCTTGG
PpuMI 1019 AGGTCCT [GA]G^G[AT]CC[CT]
1837 GGGACCT
SmlI 1147 CTCAAG C^T[CT][GA]AG
1753 CTCAAG
Tth111I 1380 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACTCAGTC
Van91I 174 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCATTGCTTGG
XmnI 37 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1605 GAAACTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII