Restriction map for
pF1KB3248
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# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MseI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AlwI 160 GATCC GGATC(4/5)
AvaI 539 CTCGAG C^[CT]CG[GA]G
AvaII 556 GGACC G^G[AT]CC
BalI 336 TGGCCA TGG^CCA
BfaI 224 CTAG C^TAG
BpmI 453 CTGGAG CTGGAG(16/14)
BseRI 55 CTCCTC GAGGAG(10/8)
BsoBI 539 CTCGAG C^[CT]CG[GA]G
BspHI 117 TCATGA T^CATGA
BstEII 6 GGTGACC G^GT[ACGT]ACC
BstF5I 75 CATCC GGATG(2/0)
BstPI 6 GGTGACC G^GT[ACGT]ACC
BstYI 159 AGATCC [GA]^GATC[CT]
Cac8I 21 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 556 GGACC G^G[ACGT]CC
DpnII 160 GATC ^GATC
DraII 555 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 336 TGGCCA [CT]^GGCC[GA]
EcoO109I 555 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 6 GGTGACC G^GT[ACGT]ACC
EcoRI 121 GAATTC G^AATTC
FokI 75 CATCC GGATG(9/13)
HaeIII 337 GGCC GG^CC
HgaI 398 GACGC GACGC(5/10)
HphI 6 GGTGA GGTGA(8/7)
MaeI 224 CTAG C^TAG
MboI 160 GATC ^GATC
MflI 159 AGATCC [GA]^GATC[CT]
MluNI 336 TGGCCA TGG^CCA
MscI 336 TGGCCA TGG^CCA
NdeII 160 GATC ^GATC
PaeR7I 539 CTCGAG C^TCGAG
PpuMI 555 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 117 TCATGA T^CATGA
Sau3AI 160 GATC ^GATC
Sau96I 556 GGACC G^G[ACGT]CC
SmlI 539 CTCGAG C^T[CT][GA]AG
XhoI 539 CTCGAG C^TCGAG
XhoII 159 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 221 GTAC GT^AC
586 GTAC
BsaJI 451 CCCTGG C^C[ACGT][ACGT]GG
559 CCCTGG
BsmFI 90 GGGAC GGGAC(10/14)
555 GGGAC
BssKI 452 CCTGG ^CC[ACGT]GG
560 CCTGG
BstNI 452 CCTGG CC^[AT]GG
560 CCTGG
DdeI 428 CTAAG C^T[ACGT]AG
611 CTCAG
EcoRII 452 CCTGG ^CC[AT]GG
560 CCTGG
MaeIII 7 GTGAC ^GT[ACGT]AC
602 GTGAC
MvaI 452 CCTGG CC^[AT]GG
560 CCTGG
MwoI 21 GCCAGCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
30 GCTGCTGCAGC
NspI 39 GCATGT [GA]CATG^[CT]
308 GCATGT
PstI 34 CTGCAG CTGCA^G
197 CTGCAG
RsaI 221 GTAC GT^AC
586 GTAC
ScrFI 452 CCTGG CC^[ACGT]GG
560 CCTGG
Tsp45I 7 GTGAC ^GT[GC]AC
602 GTGAC
TspRI 167 CACTG CA[GC]TG[ACGT][ACGT]^
613 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AcsI AluI ApoI BbvI Fnu4HI
ItaI MboII MnlI NlaIII NlaIV
SfaNI SfcI TaqI TseI Tsp509I
TthHB8I