Restriction map for
pF1KB3243
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HincII HindII
HpaI KpnI KspI MfeI MluI
MroI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 2551 GTCTAC GT^[AC][GT]AC
AcyI 3 GGCGCC G[GA]^CG[CT]C
BalI 1694 TGGCCA TGG^CCA
BbeI 3 GGCGCC GGCGC^C
BbrPI 2077 CACGTG CAC^GTG
BclI 341 TGATCA T^GATCA
BcnI 2744 CCCGG CC^[GC]GG
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BseRI 610 GAGGAG GAGGAG(10/8)
BsgI 295 GTGCAG GTGCAG(16/14)
Bsp1407I 279 TGTACA T^GTACA
BspHI 1395 TCATGA T^CATGA
BspMI 580 ACCTGC ACCTGC(4/8)
BsrGI 279 TGTACA T^GTACA
BstAPI 543 GCATGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 412 GGTGACC G^GT[ACGT]ACC
BstPI 412 GGTGACC G^GT[ACGT]ACC
DraIII 2077 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2132 CCACGG C^C[GA][CT]GG
EcoNI 2391 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 412 GGTGACC G^GT[ACGT]ACC
EcoRV 1684 GATATC GAT^ATC
FbaI 341 TGATCA T^GATCA
HgaI 1849 GACGC GACGC(5/10)
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HindIII 1969 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
MluNI 1694 TGGCCA TGG^CCA
MscI 1694 TGGCCA TGG^CCA
NarI 3 GGCGCC GG^CGCC
NciI 2744 CCCGG CC^[GC]GG
NruI 2664 TCGCGA TCG^CGA
NspI 498 GCATGC [GA]CATG^[CT]
PaeR7I 1004 CTCGAG C^TCGAG
PmaCI 2077 CACGTG CAC^GTG
PmlI 2077 CACGTG CAC^GTG
RcaI 1395 TCATGA T^CATGA
SexAI 93 ACCTGGT A^CC[AT]GGT
SfoI 3 GGCGCC GGC^GCC
SphI 498 GCATGC GCATG^C
SspBI 279 TGTACA T^GTACA
SspI 117 AATATT AAT^ATT
XbaI 1734 TCTAGA T^CTAGA
XhoI 1004 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 1826 GTGCAC G^TGCAC
2352 GTGCAC
AocI 445 CCTAAGG CC^T[ACGT]AGG
1620 CCTGAGG
ApaLI 1826 GTGCAC G^TGCAC
2352 GTGCAC
Asp700I 2268 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2518 GAATTACTTC
AvaI 358 CCCGAG C^[CT]CG[GA]G
1004 CTCGAG
AvaII 2301 GGACC G^G[AT]CC
2772 GGACC
BbsI 268 GAAGAC GAAGAC(2/6)
1738 GAAGAC
BciVI 1602 GGATAC GTATCC(6/5)
2283 GGATAC
BpuAI 268 GAAGAC GAAGAC(2/6)
1738 GAAGAC
BsaAI 1111 TACGTG [CT]AC^GT[GA]
2077 CACGTG
BsaBI 142 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
186 GATTGGGATC
BsmFI 721 GTCCC GGGAC(10/14)
2638 GTCCC
BsmI 2268 GAATGC GAATGC(1/-1)
2272 GCATTC
BsoBI 358 CCCGAG C^[CT]CG[GA]G
1004 CTCGAG
BsrFI 1094 ACCGGC [GA]^CCGG[CT]
2054 GCCGGT
BstXI 898 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2582 CCATCTGCATGG
Bsu36I 445 CCTAAGG CC^T[ACGT]AGG
1620 CCTGAGG
Cfr10I 1094 ACCGGC [GA]^CCGG[CT]
2054 GCCGGT
Eco81I 445 CCTAAGG CC^T[ACGT]AGG
1620 CCTGAGG
EcoRI 318 GAATTC G^AATTC
2246 GAATTC
EcoT14I 1188 CCTTGG C^C[AT][AT]GG
2159 CCAAGG
EcoT22I 541 ATGCAT ATGCA^T
2270 ATGCAT
HaeII 3 GGCGCC [GA]GCGC^[CT]
1267 GGCGCT
MaeII 1112 ACGT A^CGT
2078 ACGT
MamI 142 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
186 GATTGGGATC
NsiI 541 ATGCAT ATGCA^T
2270 ATGCAT
PflMI 1580 CCATAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCACGTGGTGG
PstI 1271 CTGCAG CTGCA^G
1349 CTGCAG
StyI 1188 CCTTGG C^C[AT][AT]GG
2159 CCAAGG
TaqI 701 TCGA T^CGA
1005 TCGA
TthHB8I 701 TCGA T^CGA
1005 TCGA
Van91I 1580 CCATAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCACGTGGTGG
XmnI 2268 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2518 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
BanI BanII BbvI BfaI BglII
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII