Restriction map for
pF1KB3222
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
Aor51HI ApaI AscI AseI AsnI
AviII AvrII BamHI BbeI BciVI
BfrI BglI BlnI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HpaI KasI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 35 GGTACC G^GTACC
AccII 1320 CGCG CG^CG
AclI 1633 AACGTT AA^CGTT
AcyI 729 GGCGTC G[GA]^CG[CT]C
AhdI 724 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 35 GGTACC G^GTACC
AspEI 724 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 509 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 296 CACGTG CAC^GTG
BbvCI 2379 CCTCAGC CCTCAGC(-5/-2)
BclI 824 TGATCA T^GATCA
BfaI 160 CTAG C^TAG
BglII 1796 AGATCT A^GATCT
BlpI 2748 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2748 GCTGAGC GC^T[ACGT]AGC
BsaBI 1956 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 729 GGCGTC G[GA]^CG[CT]C
BsaWI 530 ACCGGA [AT]^CCGG[AT]
BssSI 1047 CACGAG CACGAG(-5/-1)
BstUI 1320 CGCG CG^CG
CelII 2748 GCTGAGC GC^T[ACGT]AGC
Eam1105I 724 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1446 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2505 GAATTC G^AATTC
FbaI 824 TGATCA T^GATCA
HaeII 2333 GGCGCT [GA]GCGC^[CT]
Hin1I 729 GGCGTC G[GA]^CG[CT]C
KpnI 35 GGTACC GGTAC^C
KspI 1319 CCGCGG CCGC^GG
MaeI 160 CTAG C^TAG
MamI 1956 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1320 CGCG CG^CG
PflFI 509 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2391 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 296 CACGTG CAC^GTG
PmlI 296 CACGTG CAC^GTG
PshAI 2913 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1633 AACGTT AA^CGTT
PvuII 1879 CAGCTG CAG^CTG
SacII 1319 CCGCGG CCGC^GG
SmaI 1358 CCCGGG CCC^GGG
StuI 1028 AGGCCT AGG^CCT
Tth111I 509 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2391 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1046 CCACGAGACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1358 CCCGGG C^CCGGG
XmaI 1358 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 297 ACGTGT A^C[GA][CT]GT
583 ACGTGT
Alw44I 655 GTGCAC G^TGCAC
1254 GTGCAC
AocI 1464 CCTCAGG CC^T[ACGT]AGG
1506 CCTGAGG
ApaLI 655 GTGCAC G^TGCAC
1254 GTGCAC
Asp700I 1515 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2884 GAAACACTTC
BanI 35 GGTACC G^G[CT][GA]CC
925 GGTGCC
BanII 591 GAGCCC G[GA]GC[CT]^C
1402 GGGCTC
BcgI 295 GCACGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2591 GCAACACTCTCG
BsaAI 296 CACGTG [CT]AC^GT[GA]
427 TACGTG
BsaI 242 GAGACC GGTCTC(1/5)
1050 GAGACC
BsgI 954 CTGCAC GTGCAG(16/14)
2110 GTGCAG
BsmBI 514 CGTCTC CGTCTC(1/5)
1667 GAGACG
BsmI 462 GAATGC GAATGC(1/-1)
642 GCATTC
BspHI 827 TCATGA T^CATGA
2786 TCATGA
BspMI 1601 ACCTGC ACCTGC(4/8)
2582 ACCTGC
BsrBI 949 CCGCTC CCGCTC(-3/-3)
976 GAGCGG
BstAPI 14 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1074 GCAGCACTTGC
BstBI 303 TTCGAA TT^CGAA
1702 TTCGAA
Bsu36I 1464 CCTCAGG CC^T[ACGT]AGG
1506 CCTGAGG
CfoI 1388 GCGC GCG^C
2334 GCGC
DraIII 658 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
1218 CACCATGTG
DrdI 1669 GACGTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2776 GACCTGGGGGTC
Eco81I 1464 CCTCAGG CC^T[ACGT]AGG
1506 CCTGAGG
FauI 17 CCCGC CCCGC(4/6)
978 GCGGG
HgaI 513 GCGTC GACGC(5/10)
730 GCGTC
HhaI 1388 GCGC GCG^C
2334 GCGC
HinP1I 1388 GCGC G^CGC
2334 GCGC
HindIII 1654 AAGCTT A^AGCTT
2653 AAGCTT
NcoI 206 CCATGG C^CATGG
1937 CCATGG
NspI 1098 GCATGT [GA]CATG^[CT]
2057 ACATGC
NspV 303 TTCGAA TT^CGAA
1702 TTCGAA
RcaI 827 TCATGA T^CATGA
2786 TCATGA
ScaI 1139 AGTACT AGT^ACT
1151 AGTACT
SfuI 303 TTCGAA TT^CGAA
1702 TTCGAA
XmnI 1515 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2884 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII