Restriction map for
pF1KB3191
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BfrI BlnI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeIII
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1720 GACGTC GACGT^C
AflIII 1017 ACATGT A^C[GA][CT]GT
Alw44I 256 GTGCAC G^TGCAC
AocI 1132 CCTGAGG CC^T[ACGT]AGG
ApaLI 256 GTGCAC G^TGCAC
AvaI 467 CCCGAG C^[CT]CG[GA]G
AviII 1577 TGCGCA TGC^GCA
BbsI 757 GAAGAC GAAGAC(2/6)
BbvCI 1234 GCTGAGG CCTCAGC(-5/-2)
BclI 962 TGATCA T^GATCA
BcnI 1593 CCCGG CC^[GC]GG
BglI 1735 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 974 AGATCT A^GATCT
BlpI 1111 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1111 GCTCAGC GC^T[ACGT]AGC
BpuAI 757 GAAGAC GAAGAC(2/6)
BsaAI 253 TACGTG [CT]AC^GT[GA]
BsaBI 1182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 298 GAGGAG GAGGAG(10/8)
BsgI 1486 CTGCAC GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsoBI 467 CCCGAG C^[CT]CG[GA]G
BspLU11I 1017 ACATGT A^CATGT
BspMI 1484 ACCTGC ACCTGC(4/8)
BsrDI 62 GCAATG GCAATG(2/0)
BssSI 349 CACGAG CACGAG(-5/-1)
Bsu36I 1132 CCTGAGG CC^T[ACGT]AGG
CelII 1111 GCTCAGC GC^T[ACGT]AGC
DraIII 379 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1749 GACTGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
Eco81I 1132 CCTGAGG CC^T[ACGT]AGG
FbaI 962 TGATCA T^GATCA
FspI 1577 TGCGCA TGC^GCA
MamI 1182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1328 TTAA T^TAA
NciI 1593 CCCGG CC^[GC]GG
NcoI 536 CCATGG C^CATGG
PflMI 1710 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1630 CTGCAG CTGCA^G
SacI 805 GAGCTC GAGCT^C
SapI 1503 GCTCTTC GCTCTTC(1/4)
ScaI 503 AGTACT AGT^ACT
SexAI 845 ACCTGGT A^CC[AT]GGT
SfcI 1630 CTGCAG C^T[GA][CT]AG
Tru9I 1328 TTAA T^TAA
Van91I 1710 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccIII 509 TCCGGA T^CCGGA
1468 TCCGGA
AcyI 1240 GACGCC G[GA]^CG[CT]C
1720 GACGTC
AlwI 714 GGATC GGATC(4/5)
1356 GGATC
ApaI 1430 GGGCCC GGGCC^C
1642 GGGCCC
AspI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
844 GACCTGGTC
BsaHI 1240 GACGCC G[GA]^CG[CT]C
1720 GACGTC
BsaI 1472 GAGACC GGTCTC(1/5)
1051 GGTCTC
BsaWI 509 TCCGGA [AT]^CCGG[AT]
1468 TCCGGA
BseAI 509 TCCGGA T^CCGGA
1468 TCCGGA
BspEI 509 TCCGGA T^CCGGA
1468 TCCGGA
BstYI 714 GGATCT [GA]^GATC[CT]
974 AGATCT
DsaI 536 CCATGG C^C[GA][CT]GG
1568 CCACGG
HgaI 1240 GACGC GACGC(5/10)
773 GCGTC
Hin1I 1240 GACGCC G[GA]^CG[CT]C
1720 GACGTC
MflI 714 GGATCT [GA]^GATC[CT]
974 AGATCT
MroI 509 TCCGGA T^CCGGA
1468 TCCGGA
PflFI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
844 GACCTGGTC
TaqI 498 TCGA T^CGA
1085 TCGA
Tth111I 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
844 GACCTGGTC
TthHB8I 498 TCGA T^CGA
1085 TCGA
XhoII 714 GGATCT [GA]^GATC[CT]
974 AGATCT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI XcmI