##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BbrPI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BpmI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AspHI 406 GAGCTC G[AT]GC[AT]^C
AspI 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 563 GGATCC G^GATCC
BanII 406 GAGCTC G[GA]GC[CT]^C
BbsI 651 GAAGAC GAAGAC(2/6)
BcgI 84 CGAGTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 293 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 255 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 255 GCTCAGC GC^T[ACGT]AGC
BpuAI 651 GAAGAC GAAGAC(2/6)
BsiEI 194 CGGTCG CG[GA][CT]^CG
BsiHKAI 406 GAGCTC G[AT]GC[AT]^C
BsmAI 645 GTCTC GTCTC(1/5)
BspMI 551 ACCTGC ACCTGC(4/8)
BsrI 428 ACTGG ACTGG(1/-1)
BstXI 392 CCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 563 GGATCC [GA]^GATC[CT]
CelII 255 GCTCAGC GC^T[ACGT]AGC
DpnII 564 GATC ^GATC
EarI 409 CTCTTC CTCTTC(1/4)
EcoNI 288 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 484 GTCAAC GT[CT]^[GA]AC
HindII 484 GTCAAC GT[CT]^[GA]AC
HinfI 654 GACTC G^A[ACGT]TC
Ksp632I 409 CTCTTC CTCTTC(1/4)
KspI 242 CCGCGG CCGC^GG
MboI 564 GATC ^GATC
MflI 563 GGATCC [GA]^GATC[CT]
NaeI 298 GCCGGC GCC^GGC
NdeII 564 GATC ^GATC
NgoMIV 298 GCCGGC G^CCGGC
NspI 614 GCATGC [GA]CATG^[CT]
PflFI 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 654 GACTC GAGTC(4/5)
PstI 214 CTGCAG CTGCA^G
PvuII 352 CAGCTG CAG^CTG
SacI 406 GAGCTC GAGCT^C
SacII 242 CCGCGG CCGC^GG
SapI 408 GCTCTTC GCTCTTC(1/4)
Sau3AI 564 GATC ^GATC
SfcI 214 CTGCAG C^T[GA][CT]AG
SphI 614 GCATGC GCATG^C
Tth111I 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 563 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 564 GATCC GGATC(4/5)
563 GGATC
BbeI 106 GGCGCC GGCGC^C
235 GGCGCC
BmyI 160 GGGCAC G[AGT]GC[ACT]^C
406 GAGCTC
BseRI 147 CTCCTC GAGGAG(10/8)
403 GAGGAG
BsgI 355 CTGCAC GTGCAG(16/14)
553 CTGCAC
Bsp1286I 160 GGGCAC G[AGT]GC[ACT]^C
406 GAGCTC
BsrFI 298 GCCGGC [GA]^CCGG[CT]
545 ACCGGC
Cfr10I 298 GCCGGC [GA]^CCGG[CT]
545 ACCGGC
DsaI 242 CCGCGG C^C[GA][CT]GG
392 CCACGG
HaeII 106 GGCGCC [GA]GCGC^[CT]
235 GGCGCC
KasI 106 GGCGCC G^GCGCC
235 GGCGCC
MslI 393 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
468 CATGTGTGTG
NarI 106 GGCGCC GG^CGCC
235 GGCGCC
NlaIII 468 CATG CATG^
615 CATG
PpuMI 188 GGGACCC [GA]G^G[AT]CC[CT]
202 AGGACCT
SfoI 106 GGCGCC GGC^GCC
235 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BbvI BcnI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV RsaI Sau96I
ScrFI SfaNI SmaI TseI XmaCI
XmaI