Restriction map for
pF1KB3109
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbeI BbrPI
BbvCI BglI BglII BlnI BlpI
Bpu1102I BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I CelII CpoI DraI Eco47III
EcoRV EcoT22I FseI HaeII HpaI
KasI KpnI KspI MfeI MroI
MunI NarI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 1610 GACGTC GACGT^C
AccI 1520 GTCGAC GT^[AC][GT]AC
AhdI 423 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 423 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 88 TGCGCA TGC^GCA
BclI 3563 TGATCA T^GATCA
BcnI 4997 CCGGG CC^[GC]GG
BsaWI 1733 ACCGGA [AT]^CCGG[AT]
BsmBI 3418 GAGACG CGTCTC(1/5)
BspDI 2101 ATCGAT AT^CGAT
BsrBI 1714 GAGCGG CCGCTC(-3/-3)
BssHII 57 GCGCGC G^CGCGC
ClaI 2101 ATCGAT AT^CGAT
DrdI 4342 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 423 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 388 GAATTC G^AATTC
FbaI 3563 TGATCA T^GATCA
FspI 88 TGCGCA TGC^GCA
HincII 1520 GTCGAC GT[CT]^[GA]AC
HindII 1520 GTCGAC GT[CT]^[GA]AC
HindIII 145 AAGCTT A^AGCTT
MluI 4928 ACGCGT A^CGCGT
NciI 4997 CCGGG CC^[GC]GG
NdeI 231 CATATG CA^TATG
NheI 18 GCTAGC G^CTAGC
PvuI 454 CGATCG CGAT^CG
SalI 1520 GTCGAC G^TCGAC
SphI 3314 GCATGC GCATG^C
XbaI 2217 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 1195 ACGTGT A^C[GA][CT]GT
4928 ACGCGT
Alw44I 2417 GTGCAC G^TGCAC
3492 GTGCAC
AocI 2329 CCTCAGG CC^T[ACGT]AGG
2876 CCTCAGG
ApaLI 2417 GTGCAC G^TGCAC
3492 GTGCAC
BanI 3100 GGCACC G^G[CT][GA]CC
3946 GGTGCC
BsaAI 1194 TACGTG [CT]AC^GT[GA]
4640 TACGTG
BsaBI 247 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
753 GATGGAAATC
BsmI 2320 GAATGC GAATGC(1/-1)
4005 GAATGC
BspHI 255 TCATGA T^CATGA
4052 TCATGA
BstAPI 3341 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3527 GCACACCCTGC
BstEII 153 GGTGACC G^GT[ACGT]ACC
2175 GGTCACC
BstPI 153 GGTGACC G^GT[ACGT]ACC
2175 GGTCACC
Bsu36I 2329 CCTCAGG CC^T[ACGT]AGG
2876 CCTCAGG
DraIII 3425 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3479 CACGCTGTG
EagI 132 CGGCCG C^GGCCG
2002 CGGCCG
EarI 3358 CTCTTC CTCTTC(1/4)
5128 GAAGAG
EclXI 132 CGGCCG C^GGCCG
2002 CGGCCG
Eco52I 132 CGGCCG C^GGCCG
2002 CGGCCG
Eco81I 2329 CCTCAGG CC^T[ACGT]AGG
2876 CCTCAGG
EcoNI 967 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3060 CCTCCGGCAGG
EcoO65I 153 GGTGACC G^GT[ACGT]ACC
2175 GGTCACC
Ksp632I 3358 CTCTTC CTCTTC(1/4)
5128 GAAGAG
MamI 247 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
753 GATGGAAATC
PflMI 2632 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3209 CCAATACCTGG
RcaI 255 TCATGA T^CATGA
4052 TCATGA
SexAI 1632 ACCTGGT A^CC[AT]GGT
1955 ACCAGGT
Van91I 2632 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3209 CCAATACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BcgI BciVI
BfaI BfrI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI