Restriction map for
pF1KB3104
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AocI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspDI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MscI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 456 GTATAC GT^[AC][GT]AC
AccIII 735 TCCGGA T^CCGGA
AcyI 1048 GACGCC G[GA]^CG[CT]C
AgeI 2321 ACCGGT A^CCGGT
ApaI 555 GGGCCC GGGCC^C
Asp700I 2191 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1702 GGCACC G^G[CT][GA]CC
BclI 2168 TGATCA T^GATCA
BglI 557 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1048 GACGCC G[GA]^CG[CT]C
BseAI 735 TCCGGA T^CCGGA
BsiEI 1797 CGGCCG CG[GA][CT]^CG
BsmI 2347 GAATGC GAATGC(1/-1)
BspEI 735 TCCGGA T^CCGGA
BspHI 243 TCATGA T^CATGA
BsrDI 527 GCAATG GCAATG(2/0)
Bst1107I 456 GTATAC GTA^TAC
BstZ17I 456 GTATAC GTA^TAC
DraIII 1834 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1797 CGGCCG C^GGCCG
EclXI 1797 CGGCCG C^GGCCG
Eco52I 1797 CGGCCG C^GGCCG
EcoRI 277 GAATTC G^AATTC
FbaI 2168 TGATCA T^GATCA
Hin1I 1048 GACGCC G[GA]^CG[CT]C
HindIII 535 AAGCTT A^AGCTT
MfeI 104 CAATTG C^AATTG
MroI 735 TCCGGA T^CCGGA
MunI 104 CAATTG C^AATTG
PinAI 2321 ACCGGT A^CCGGT
PvuII 571 CAGCTG CAG^CTG
RcaI 243 TCATGA T^CATGA
SacI 793 GAGCTC GAGCT^C
ScaI 953 AGTACT AGT^ACT
SexAI 1406 ACCTGGT A^CC[AT]GGT
SfiI 556 GGCCCTAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1707 CCCGGG CCC^GGG
SmlI 1468 CTCAAG C^T[CT][GA]AG
StuI 1094 AGGCCT AGG^CCT
XcmI 905 CCACCCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1707 CCCGGG C^CCGGG
XmaI 1707 CCCGGG C^CCGGG
XmnI 2191 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 608 GTGCAC G^TGCAC
1504 GTGCAC
Aor51HI 22 AGCGCT AGC^GCT
1432 AGCGCT
ApaLI 608 GTGCAC G^TGCAC
1504 GTGCAC
AvaI 250 CCCGAG C^[CT]CG[GA]G
1707 CCCGGG
BamHI 495 GGATCC G^GATCC
2357 GGATCC
BanII 555 GGGCCC G[GA]GC[CT]^C
793 GAGCTC
BbsI 229 GAAGAC GAAGAC(2/6)
708 GAAGAC
BcgI 743 GCACGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
986 GCACCAAGGTCG
BfaI 1285 CTAG C^TAG
1540 CTAG
BglII 216 AGATCT A^GATCT
1310 AGATCT
BpuAI 229 GAAGAC GAAGAC(2/6)
708 GAAGAC
BsaI 639 GGTCTC GGTCTC(1/5)
731 GGTCTC
BsaWI 735 TCCGGA [AT]^CCGG[AT]
2321 ACCGGT
BsmBI 1344 GAGACG CGTCTC(1/5)
1750 GAGACG
BsoBI 250 CCCGAG C^[CT]CG[GA]G
1707 CCCGGG
BspLU11I 612 ACATGT A^CATGT
1554 ACATGT
BsrFI 808 ACCGGC [GA]^CCGG[CT]
2321 ACCGGT
BssSI 744 CACGAG CACGAG(-5/-1)
1078 CACGAG
BstXI 629 CCATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1364 CCAACACGGTGG
Cac8I 1650 GCCAGC GC[ACGT]^[ACGT]GC
1856 GCTTGC
Cfr10I 808 ACCGGC [GA]^CCGG[CT]
2321 ACCGGT
EarI 220 CTCTTC CTCTTC(1/4)
694 GAAGAG
Eco47III 22 AGCGCT AGC^GCT
1432 AGCGCT
HaeII 22 AGCGCT [GA]GCGC^[CT]
1432 AGCGCT
HgaI 1048 GACGC GACGC(5/10)
1923 GACGC
Ksp632I 220 CTCTTC CTCTTC(1/4)
694 GAAGAG
MaeI 1285 CTAG C^TAG
1540 CTAG
NcoI 965 CCATGG C^CATGG
2091 CCATGG
NspI 612 ACATGT [GA]CATG^[CT]
1554 ACATGT
PflMI 909 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCATAGCATGG
PleI 749 GACTC GAGTC(4/5)
2138 GAGTC
PpuMI 1331 AGGACCT [GA]G^G[AT]CC[CT]
1479 AGGTCCT
TaqI 1736 TCGA T^CGA
2136 TCGA
TfiI 367 GAATC G^A[AT]TC
772 GATTC
TthHB8I 1736 TCGA T^CGA
2136 TCGA
Van91I 909 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCATAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII