Restriction map for
pF1KB3098
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AfaI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI Asp718I AspEI AviII AvrII
BamHI BbrPI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BstBI BstEII BstPI BstXI
CelII ClaI CpoI DraIII Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NcoI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 882 GTATAC GT^[AC][GT]AC
AccIII 717 TCCGGA T^CCGGA
AcyI 289 GGCGCC G[GA]^CG[CT]C
AocI 740 CCTCAGG CC^T[ACGT]AGG
ApaI 214 GGGCCC GGGCC^C
AscI 465 GGCGCGCC GG^CGCGCC
AseI 703 ATTAAT AT^TAAT
AsnI 703 ATTAAT AT^TAAT
AspHI 1832 GAGCAC G[AT]GC[AT]^C
AspI 746 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1853 TGGCCA TGG^CCA
BbeI 289 GGCGCC GGCGC^C
BbvCI 1822 GCTGAGG CCTCAGC(-5/-2)
BclI 1107 TGATCA T^GATCA
BsaHI 289 GGCGCC G[GA]^CG[CT]C
BseAI 717 TCCGGA T^CCGGA
BsiEI 499 CGGCCG CG[GA][CT]^CG
BsiHKAI 1832 GAGCAC G[AT]GC[AT]^C
BsmAI 1340 GAGAC GTCTC(1/5)
BsmBI 1340 GAGACG CGTCTC(1/5)
BsmFI 876 GTCCC GGGAC(10/14)
BspEI 717 TCCGGA T^CCGGA
BspMI 837 ACCTGC ACCTGC(4/8)
BsrDI 1678 GCAATG GCAATG(2/0)
BssHII 466 GCGCGC G^CGCGC
BssSI 336 CTCGTG CACGAG(-5/-1)
Bst1107I 882 GTATAC GTA^TAC
BstAPI 1810 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 882 GTATAC GTA^TAC
Bsu36I 740 CCTCAGG CC^T[ACGT]AGG
DraII 272 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 746 GACAGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 499 CGGCCG C^GGCCG
EclXI 499 CGGCCG C^GGCCG
Eco52I 499 CGGCCG C^GGCCG
Eco81I 740 CCTCAGG CC^T[ACGT]AGG
EcoO109I 272 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 966 ATGCAT ATGCA^T
FbaI 1107 TGATCA T^GATCA
FseI 239 GGCCGGCC GGCCGG^CC
HgaI 445 GCGTC GACGC(5/10)
Hin1I 289 GGCGCC G[GA]^CG[CT]C
KasI 289 GGCGCC G^GCGCC
MaeII 769 ACGT A^CGT
MluNI 1853 TGGCCA TGG^CCA
MroI 717 TCCGGA T^CCGGA
MscI 1853 TGGCCA TGG^CCA
MslI 1066 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 289 GGCGCC GG^CGCC
NdeI 1111 CATATG CA^TATG
NsiI 966 ATGCAT ATGCA^T
NspI 1395 GCATGT [GA]CATG^[CT]
PflFI 746 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 272 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 703 ATTAAT AT^TAAT
PvuII 1820 CAGCTG CAG^CTG
SfoI 289 GGCGCC GGC^GCC
Tth111I 746 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1717 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 67 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
90 CAGCGACTG
BanI 289 GGCGCC G^G[CT][GA]CC
514 GGCACC
BbsI 793 GAAGAC GAAGAC(2/6)
1667 GAAGAC
BciVI 645 GGATAC GTATCC(6/5)
1598 GGATAC
BglI 43 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
470 GCCGGGCGGGC
BpuAI 793 GAAGAC GAAGAC(2/6)
1667 GAAGAC
BsaWI 717 TCCGGA [AT]^CCGG[AT]
1847 ACCGGA
BsgI 54 CTGCAC GTGCAG(16/14)
1727 GTGCAG
BspHI 897 TCATGA T^CATGA
1181 TCATGA
BstNI 399 CCTGG CC^[AT]GG
436 CCAGG
DraI 1192 TTTAAA TTT^AAA
1375 TTTAAA
EcoRII 399 CCTGG ^CC[AT]GG
436 CCAGG
HaeII 221 AGCGCC [GA]GCGC^[CT]
289 GGCGCC
HphI 790 GGTGA GGTGA(8/7)
1384 GGTGA
KspI 451 CCGCGG CCGC^GG
496 CCGCGG
MaeIII 1187 GTTAC ^GT[ACGT]AC
1399 GTAAC
MvaI 399 CCTGG CC^[AT]GG
436 CCAGG
NaeI 240 GCCGGC GCC^GGC
381 GCCGGC
NgoMIV 240 GCCGGC G^CCGGC
381 GCCGGC
PstI 87 CTGCAG CTGCA^G
1817 CTGCAG
RcaI 897 TCATGA T^CATGA
1181 TCATGA
SacII 451 CCGCGG CCGC^GG
496 CCGCGG
SgrAI 380 CGCCGGCG C[GA]^CCGG[CT]G
416 CGCCGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI Asp700I AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MboI MboII MflI MnlI MseI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI