Restriction map for
pF1KB3090
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MslI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 150 GTCTAC GT^[AC][GT]AC
AcsI 753 AAATTC [GA]^AATT[CT]
AflIII 1417 ACATGT A^C[GA][CT]GT
ApoI 753 AAATTC [GA]^AATT[CT]
AspI 235 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 364 GGATCC G^GATCC
BanI 177 GGTGCC G^G[CT][GA]CC
BbsI 499 GAAGAC GAAGAC(2/6)
BcnI 173 CCGGG CC^[GC]GG
BmyI 1784 GTGCCC G[AGT]GC[ACT]^C
BpuAI 499 GAAGAC GAAGAC(2/6)
BsaI 1217 GAGACC GGTCTC(1/5)
BsaWI 719 ACCGGA [AT]^CCGG[AT]
BsmBI 383 CGTCTC CGTCTC(1/5)
BsmFI 35 GTCCC GGGAC(10/14)
Bsp1286I 1784 GTGCCC G[AGT]GC[ACT]^C
BspHI 1166 TCATGA T^CATGA
BspLU11I 1417 ACATGT A^CATGT
BssSI 203 CTCGTG CACGAG(-5/-1)
BstF5I 699 GGATG GGATG(2/0)
BstYI 364 GGATCC [GA]^GATC[CT]
DraI 1446 TTTAAA TTT^AAA
DsaI 964 CCATGG C^C[GA][CT]GG
EcoRV 1052 GATATC GAT^ATC
FauI 169 CCCGC CCCGC(4/6)
FokI 699 GGATG GGATG(9/13)
HincII 1080 GTCAAC GT[CT]^[GA]AC
HindII 1080 GTCAAC GT[CT]^[GA]AC
MaeII 382 ACGT A^CGT
MflI 364 GGATCC [GA]^GATC[CT]
MspA1I 1072 CAGCTG C[AC]G^C[GT]G
NciI 173 CCGGG CC^[GC]GG
NcoI 964 CCATGG C^CATGG
NdeI 574 CATATG CA^TATG
PflFI 235 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1072 CAGCTG CAG^CTG
RcaI 1166 TCATGA T^CATGA
ScaI 544 AGTACT AGT^ACT
SexAI 1220 ACCTGGT A^CC[AT]GGT
SmlI 936 CTTGAG C^T[CT][GA]AG
SphI 794 GCATGC GCATG^C
Tth111I 235 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 364 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 170 CCGC CCGC(-3/-1)
1558 GCGG
AlwNI 340 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
681 CAGTAACTG
AseI 1377 ATTAAT AT^TAAT
1462 ATTAAT
AsnI 1377 ATTAAT AT^TAAT
1462 ATTAAT
BalI 580 TGGCCA TGG^CCA
835 TGGCCA
BseRI 1330 GAGGAG GAGGAG(10/8)
1686 GAGGAG
BsrDI 538 GCAATG GCAATG(2/0)
899 GCAATG
DraII 33 AGGTCCC [GA]G^G[ACGT]CC[CT]
636 AGGACCT
EarI 507 CTCTTC CTCTTC(1/4)
1327 GAAGAG
EcoO109I 33 AGGTCCC [GA]G^G[ACGT]CC[CT]
636 AGGACCT
HapII 173 CCGG C^CGG
720 CCGG
HpaII 173 CCGG C^CGG
720 CCGG
HphI 1224 GGTGA GGTGA(8/7)
598 TCACC
Ksp632I 507 CTCTTC CTCTTC(1/4)
1327 GAAGAG
MfeI 1411 CAATTG C^AATTG
1681 CAATTG
MluNI 580 TGGCCA TGG^CCA
835 TGGCCA
MscI 580 TGGCCA TGG^CCA
835 TGGCCA
MspI 173 CCGG C^CGG
720 CCGG
MunI 1411 CAATTG C^AATTG
1681 CAATTG
PleI 1594 GACTC GAGTC(4/5)
195 GAGTC
PpuMI 33 AGGTCCC [GA]G^G[AT]CC[CT]
636 AGGACCT
PshBI 1377 ATTAAT AT^TAAT
1462 ATTAAT
TaqI 514 TCGA T^CGA
813 TCGA
Tsp45I 597 GTCAC ^GT[GC]AC
1668 GTCAC
TthHB8I 514 TCGA T^CGA
813 TCGA
XbaI 487 TCTAGA T^CTAGA
759 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI