Restriction map for
pF1KB3088
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbsI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BsrBI
BsrFI BssSI BstBI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HpaI KpnI
MaeI MaeII MamI MfeI MluI
MluNI MscI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 886 GTATAC GT^[AC][GT]AC
AccIII 477 TCCGGA T^CCGGA
Asp700I 1144 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 725 GTGCTC G[AT]GC[AT]^C
AvaI 1079 CTCGAG C^[CT]CG[GA]G
BamHI 474 GGATCC G^GATCC
BanII 235 GAGCCC G[GA]GC[CT]^C
BbvCI 60 GCTGAGG CCTCAGC(-5/-2)
BglI 155 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 477 TCCGGA [AT]^CCGG[AT]
BseAI 477 TCCGGA T^CCGGA
BsiHKAI 725 GTGCTC G[AT]GC[AT]^C
BsmAI 489 GTCTC GTCTC(1/5)
BsoBI 1079 CTCGAG C^[CT]CG[GA]G
Bsp1407I 680 TGTACA T^GTACA
BspEI 477 TCCGGA T^CCGGA
BspMI 80 ACCTGC ACCTGC(4/8)
BsrGI 680 TGTACA T^GTACA
BssHII 145 GCGCGC G^CGCGC
Bst1107I 886 GTATAC GTA^TAC
BstAPI 1084 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 747 GGTAACC G^GT[ACGT]ACC
BstPI 747 GGTAACC G^GT[ACGT]ACC
BstXI 1095 CCACGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 886 GTATAC GTA^TAC
EaeI 153 CGGCCA [CT]^GGCC[GA]
EarI 1000 GAAGAG CTCTTC(1/4)
EcoNI 114 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 747 GGTAACC G^GT[ACGT]ACC
HgaI 289 GACGC GACGC(5/10)
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
Ksp632I 1000 GAAGAG CTCTTC(1/4)
MroI 477 TCCGGA T^CCGGA
NspI 537 GCATGT [GA]CATG^[CT]
PaeR7I 1079 CTCGAG C^TCGAG
ScaI 1043 AGTACT AGT^ACT
SspBI 680 TGTACA T^GTACA
XhoI 1079 CTCGAG C^TCGAG
XmnI 1144 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 184 GGCGCC G[GA]^CG[CT]C
279 GGCGCC
AfaI 681 GTAC GT^AC
1044 GTAC
AlwNI 165 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1085 CAGCTTCTG
BbeI 184 GGCGCC GGCGC^C
279 GGCGCC
BclI 417 TGATCA T^GATCA
546 TGATCA
BcnI 15 CCCGG CC^[GC]GG
194 CCGGG
BmyI 235 GAGCCC G[AGT]GC[ACT]^C
725 GTGCTC
BsaHI 184 GGCGCC G[GA]^CG[CT]C
279 GGCGCC
BsgI 347 GTGCAG GTGCAG(16/14)
1010 GTGCAG
Bsp1286I 235 GAGCCC G[AGT]GC[ACT]^C
725 GTGCTC
BsrDI 909 CATTGC GCAATG(2/0)
1123 GCAATG
BsrI 669 ACTGG ACTGG(1/-1)
310 CCAGT
BstYI 11 AGATCC [GA]^GATC[CT]
474 GGATCC
DsaI 74 CCGCGG C^C[GA][CT]GG
284 CCGCGG
FbaI 417 TGATCA T^GATCA
546 TGATCA
HaeII 184 GGCGCC [GA]GCGC^[CT]
279 GGCGCC
HaeIII 154 GGCC GG^CC
672 GGCC
Hin1I 184 GGCGCC G[GA]^CG[CT]C
279 GGCGCC
HindIII 353 AAGCTT A^AGCTT
804 AAGCTT
KasI 184 GGCGCC G^GCGCC
279 GGCGCC
KspI 74 CCGCGG CCGC^GG
284 CCGCGG
MflI 11 AGATCC [GA]^GATC[CT]
474 GGATCC
NarI 184 GGCGCC GG^CGCC
279 GGCGCC
NciI 15 CCCGG CC^[GC]GG
194 CCGGG
RsaI 681 GTAC GT^AC
1044 GTAC
SacII 74 CCGCGG CCGC^GG
284 CCGCGG
SfoI 184 GGCGCC GGC^GCC
279 GGCGCC
SmlI 491 CTCAAG C^T[CT][GA]AG
1079 CTCGAG
XhoII 11 AGATCC [GA]^GATC[CT]
474 GGATCC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BbvI BpmI BsaJI BseRI
BsiYI BslI BsmFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I