Restriction map for
pF1KB3078
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbsI BbvCI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrGI BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 2086 GTCTAC GT^[AC][GT]AC
AcsI 1279 AAATTT [GA]^AATT[CT]
Alw44I 877 GTGCAC G^TGCAC
ApaI 1048 GGGCCC GGGCC^C
ApaLI 877 GTGCAC G^TGCAC
ApoI 1279 AAATTT [GA]^AATT[CT]
Asp700I 342 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 2066 GCAAGTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1829 GTATCC GTATCC(6/5)
BclI 1478 TGATCA T^GATCA
BfaI 487 CTAG C^TAG
BglI 2360 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1427 AGATCT A^GATCT
BsaBI 2575 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 805 GAGGAG GAGGAG(10/8)
BsrDI 423 CATTGC GCAATG(2/0)
BsrFI 1139 GCCGGC [GA]^CCGG[CT]
BssHII 61 GCGCGC G^CGCGC
Cfr10I 1139 GCCGGC [GA]^CCGG[CT]
CpoI 520 CGGACCG CG^G[AT]CCG
DraI 2559 TTTAAA TTT^AAA
DraIII 315 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 142 CGGCCG C^GGCCG
EclXI 142 CGGCCG C^GGCCG
Eco52I 142 CGGCCG C^GGCCG
EcoRV 1912 GATATC GAT^ATC
FbaI 1478 TGATCA T^GATCA
KspI 10 CCGCGG CCGC^GG
MaeI 487 CTAG C^TAG
MamI 2575 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1139 GCCGGC GCC^GGC
NdeI 1954 CATATG CA^TATG
NgoMIV 1139 GCCGGC G^CCGGC
PpuMI 1666 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 520 CGGACCG CG^G[AT]CCG
SacII 10 CCGCGG CCGC^GG
SmlI 568 CTCAAG C^T[CT][GA]AG
XcmI 2717 CCATCGGAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 342 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 908 TCCGGA T^CCGGA
1250 TCCGGA
AflIII 812 ACATGT A^C[GA][CT]GT
2784 ACATGT
BbeI 105 GGCGCC GGCGC^C
133 GGCGCC
BseAI 908 TCCGGA T^CCGGA
1250 TCCGGA
BsgI 454 GTGCAG GTGCAG(16/14)
2155 GTGCAG
BsiEI 142 CGGCCG CG[GA][CT]^CG
1694 CGACCG
BspEI 908 TCCGGA T^CCGGA
1250 TCCGGA
BspLU11I 812 ACATGT A^CATGT
2784 ACATGT
BspMI 299 ACCTGC ACCTGC(4/8)
873 GCAGGT
BsrBI 163 CCGCTC CCGCTC(-3/-3)
187 CCGCTC
BstEII 2328 GGTCACC G^GT[ACGT]ACC
2433 GGTCACC
BstPI 2328 GGTCACC G^GT[ACGT]ACC
2433 GGTCACC
BstXI 194 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1403 CCATCATCCTGG
EarI 1498 CTCTTC CTCTTC(1/4)
2428 GAAGAG
EcoO65I 2328 GGTCACC G^GT[ACGT]ACC
2433 GGTCACC
HaeII 105 GGCGCC [GA]GCGC^[CT]
133 GGCGCC
KasI 105 GGCGCC G^GCGCC
133 GGCGCC
Ksp632I 1498 CTCTTC CTCTTC(1/4)
2428 GAAGAG
MroI 908 TCCGGA T^CCGGA
1250 TCCGGA
NarI 105 GGCGCC GG^CGCC
133 GGCGCC
NcoI 929 CCATGG C^CATGG
1115 CCATGG
SfoI 105 GGCGCC GGC^GCC
133 GGCGCC
SphI 1128 GCATGC GCATG^C
1808 GCATGC
Tsp509I 1280 AATT ^AATT
1289 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI