Restriction map for
pF1KB3051
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AocI AseI AsnI Asp700I AviII
AvrII BamHI BbrPI BcgI BfaI
BfrI BlnI BsaWI BseAI BsiWI
BspDI BspEI BspLU11I Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI MaeI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 2967 AAATTC [GA]^AATT[CT]
AhdI 1897 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1394 GTGCAC G^TGCAC
Aor51HI 1789 AGCGCT AGC^GCT
ApaI 1076 GGGCCC GGGCC^C
ApaLI 1394 GTGCAC G^TGCAC
ApoI 2967 AAATTC [GA]^AATT[CT]
AscI 2911 GGCGCGCC GG^CGCGCC
AspEI 1897 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2000 GGCGCC GGCGC^C
BbvCI 2602 GCTGAGG CCTCAGC(-5/-2)
BciVI 205 GGATAC GTATCC(6/5)
BglII 3446 AGATCT A^GATCT
BlpI 3081 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3081 GCTCAGC GC^T[ACGT]AGC
BsaAI 1147 CACGTA [CT]AC^GT[GA]
BsmBI 3177 CGTCTC CGTCTC(1/5)
BspHI 452 TCATGA T^CATGA
BssHII 2912 GCGCGC G^CGCGC
CelII 3081 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1897 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1789 AGCGCT AGC^GCT
EcoNI 479 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 3571 GTCGAC GT[CT]^[GA]AC
HindII 3571 GTCGAC GT[CT]^[GA]AC
HindIII 1750 AAGCTT A^AGCTT
KasI 2000 GGCGCC G^GCGCC
NarI 2000 GGCGCC GG^CGCC
NcoI 2504 CCATGG C^CATGG
RcaI 452 TCATGA T^CATGA
SalI 3571 GTCGAC G^TCGAC
SfiI 1821 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2000 GGCGCC GGC^GCC
SphI 878 GCATGC GCATG^C
StuI 1857 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AatII 319 GACGTC GACGT^C
687 GACGTC
Acc65I 1165 GGTACC G^GTACC
2075 GGTACC
Asp718I 1165 GGTACC G^GTACC
2075 GGTACC
BbsI 993 GAAGAC GAAGAC(2/6)
1930 GTCTTC
BclI 503 TGATCA T^GATCA
3329 TGATCA
BpuAI 993 GAAGAC GAAGAC(2/6)
1930 GTCTTC
BsaBI 927 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1119 GATGGAGATC
BsaI 434 GAGACC GGTCTC(1/5)
2473 GAGACC
BsmI 2349 GAATGC GAATGC(1/-1)
3413 GCATTC
Bsp1407I 509 TGTACA T^GTACA
3503 TGTACA
BsrDI 3255 CATTGC GCAATG(2/0)
976 GCAATG
BsrGI 509 TGTACA T^GTACA
3503 TGTACA
BssSI 1936 CACGAG CACGAG(-5/-1)
3283 CACGAG
BstXI 431 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3035 CCATCGTGCTGG
EagI 1966 CGGCCG C^GGCCG
3118 CGGCCG
EclXI 1966 CGGCCG C^GGCCG
3118 CGGCCG
Eco52I 1966 CGGCCG C^GGCCG
3118 CGGCCG
FbaI 503 TGATCA T^GATCA
3329 TGATCA
KpnI 1165 GGTACC GGTAC^C
2075 GGTACC
KspI 66 CCGCGG CCGC^GG
757 CCGCGG
MamI 927 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1119 GATGGAGATC
MseI 922 TTAA T^TAA
2978 TTAA
NspI 35 GCATGT [GA]CATG^[CT]
878 GCATGC
SacII 66 CCGCGG CCGC^GG
757 CCGCGG
SapI 1987 GAAGAGC GCTCTTC(1/4)
2517 GAAGAGC
SmaI 589 CCCGGG CCC^GGG
2088 CCCGGG
SspBI 509 TGTACA T^GTACA
3503 TGTACA
TfiI 48 GAATC G^A[AT]TC
3432 GAATC
Tru9I 922 TTAA T^TAA
2978 TTAA
XmaCI 589 CCCGGG C^CCGGG
2088 CCCGGG
XmaI 589 CCCGGG C^CCGGG
2088 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII