Restriction map for
pF1KB3048
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# NO CUT ENZYMES ( 77 enzymes) #
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AclI AflII AgeI AhdI AscI
Asp700I AspEI AspI AvrII BbeI
BbrPI BclI BfrI BglII BlnI
BsmBI BspHI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstPI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoT22I FbaI
FseI HpaI KasI KspI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1756 GACGTC GACGT^C
AccII 2994 CGCG CG^CG
Alw44I 2169 GTGCAC G^TGCAC
AlwNI 2931 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1746 CCTGAGG CC^T[ACGT]AGG
Aor51HI 732 AGCGCT AGC^GCT
ApaLI 2169 GTGCAC G^TGCAC
AvaI 1223 CCCGGG C^[CT]CG[GA]G
AviII 2005 TGCGCA TGC^GCA
BamHI 996 GGATCC G^GATCC
BbvCI 3727 GCTGAGG CCTCAGC(-5/-2)
BcgI 967 CGATGTACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3583 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3583 GCTCAGC GC^T[ACGT]AGC
BsaI 2330 GAGACC GGTCTC(1/5)
BsiEI 3385 CGACCG CG[GA][CT]^CG
BsiWI 2581 CGTACG C^GTACG
BsoBI 1223 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2983 TGTACA T^GTACA
BspDI 965 ATCGAT AT^CGAT
BsrGI 2983 TGTACA T^GTACA
BstUI 2994 CGCG CG^CG
BstXI 3350 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1746 CCTGAGG CC^T[ACGT]AGG
CelII 3583 GCTCAGC GC^T[ACGT]AGC
ClaI 965 ATCGAT AT^CGAT
Eco47III 732 AGCGCT AGC^GCT
Eco81I 1746 CCTGAGG CC^T[ACGT]AGG
EcoRI 1905 GAATTC G^AATTC
FspI 2005 TGCGCA TGC^GCA
HaeII 732 AGCGCT [GA]GCGC^[CT]
MvnI 2994 CGCG CG^CG
NdeI 3623 CATATG CA^TATG
SapI 1195 GAAGAGC GCTCTTC(1/4)
SfiI 535 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1223 CCCGGG CCC^GGG
SphI 3539 GCATGC GCATG^C
SspBI 2983 TGTACA T^GTACA
XcmI 702 CCAGGAAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1223 CCCGGG C^CCGGG
XmaI 1223 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 2076 TCCGGA T^CCGGA
2427 TCCGGA
ApaI 1051 GGGCCC GGGCC^C
1152 GGGCCC
AspHI 2169 GTGCAC G[AT]GC[AT]^C
2252 GTGCTC
BglI 536 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2054 GCCACAAAGGC
BsaBI 2875 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3808 GATGGGTATC
BseAI 2076 TCCGGA T^CCGGA
2427 TCCGGA
BsiHKAI 2169 GTGCAC G[AT]GC[AT]^C
2252 GTGCTC
BspEI 2076 TCCGGA T^CCGGA
2427 TCCGGA
BspLU11I 2042 ACATGT A^CATGT
2458 ACATGT
BsrBI 46 CCGCTC CCGCTC(-3/-3)
2844 CCGCTC
Bst1107I 3380 GTATAC GTA^TAC
3660 GTATAC
BstZ17I 3380 GTATAC GTA^TAC
3660 GTATAC
EarI 1042 GAAGAG CTCTTC(1/4)
1195 GAAGAG
EcoRV 1507 GATATC GAT^ATC
2344 GATATC
HindIII 497 AAGCTT A^AGCTT
1430 AAGCTT
Ksp632I 1042 GAAGAG CTCTTC(1/4)
1195 GAAGAG
MamI 2875 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3808 GATGGGTATC
MroI 2076 TCCGGA T^CCGGA
2427 TCCGGA
NaeI 3473 GCCGGC GCC^GGC
3614 GCCGGC
NgoMIV 3473 GCCGGC G^CCGGC
3614 GCCGGC
PpuMI 2407 GGGTCCT [GA]G^G[AT]CC[CT]
2782 GGGTCCT
SmlI 1315 CTTGAG C^T[CT][GA]AG
1469 CTCAAG
StuI 1145 AGGCCT AGG^CCT
3530 AGGCCT
TaqI 966 TCGA T^CGA
3845 TCGA
TthHB8I 966 TCGA T^CGA
3845 TCGA
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AseI AsnI Asp718I AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshAI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII