Restriction map for
pF1KB3047
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI AhdI AocI Aor51HI AscI
Asp718I AspEI AspI BalI BanI
BbeI BcgI BfrI BglI BlpI
Bpu1102I BsaHI BsaI BsgI BsiEI
BsiWI BspDI BsrBI BsrFI BssHII
BssSI Bst1107I BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FseI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PstI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 2771 TCCGGA T^CCGGA
Alw44I 2462 GTGCAC G^TGCAC
ApaI 2535 GGGCCC GGGCC^C
ApaLI 2462 GTGCAC G^TGCAC
AseI 2205 ATTAAT AT^TAAT
AsnI 2205 ATTAAT AT^TAAT
Asp700I 3425 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2321 CTCGGG C^[CT]CG[GA]G
AviII 2802 TGCGCA TGC^GCA
AvrII 850 CCTAGG C^CTAGG
BbrPI 2668 CACGTG CAC^GTG
BbvCI 1678 GCTGAGG CCTCAGC(-5/-2)
BciVI 2002 GGATAC GTATCC(6/5)
BclI 3353 TGATCA T^GATCA
BlnI 850 CCTAGG C^CTAGG
BsaBI 2257 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2771 TCCGGA [AT]^CCGG[AT]
BseAI 2771 TCCGGA T^CCGGA
BsmBI 223 GAGACG CGTCTC(1/5)
BsmI 2022 GAATGC GAATGC(1/-1)
BsoBI 2321 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3015 TGTACA T^GTACA
BspEI 2771 TCCGGA T^CCGGA
BspHI 2622 TCATGA T^CATGA
BspLU11I 2588 ACATGT A^CATGT
BsrGI 3015 TGTACA T^GTACA
BstAPI 2706 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3432 TTCGAA TT^CGAA
BstXI 1172 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2803 GCGC GCG^C
EcoRI 2195 GAATTC G^AATTC
FauI 739 GCGGG CCCGC(4/6)
FbaI 3353 TGATCA T^GATCA
FspI 2802 TGCGCA TGC^GCA
HhaI 2803 GCGC GCG^C
HinP1I 2803 GCGC G^CGC
HindIII 1139 AAGCTT A^AGCTT
MamI 2257 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2771 TCCGGA T^CCGGA
NspV 3432 TTCGAA TT^CGAA
PmaCI 2668 CACGTG CAC^GTG
PmlI 2668 CACGTG CAC^GTG
PshBI 2205 ATTAAT AT^TAAT
RcaI 2622 TCATGA T^CATGA
SacI 981 GAGCTC GAGCT^C
ScaI 492 AGTACT AGT^ACT
SexAI 282 ACCTGGT A^CC[AT]GGT
SfuI 3432 TTCGAA TT^CGAA
SmlI 1783 CTCAAG C^T[CT][GA]AG
SspBI 3015 TGTACA T^GTACA
StuI 1224 AGGCCT AGG^CCT
XbaI 3244 TCTAGA T^CTAGA
XmnI 3425 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2898 GTCTAC GT^[AC][GT]AC
3040 GTAGAC
AclI 747 AACGTT AA^CGTT
1292 AACGTT
AflIII 2588 ACATGT A^C[GA][CT]GT
2667 ACACGT
AlwNI 2926 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
3120 CAGCAGCTG
AspHI 981 GAGCTC G[AT]GC[AT]^C
2462 GTGCAC
AvaII 1168 GGTCC G^G[AT]CC
2928 GGACC
BamHI 303 GGATCC G^GATCC
3159 GGATCC
BbsI 3094 GAAGAC GAAGAC(2/6)
2792 GTCTTC
BcnI 2036 CCCGG CC^[GC]GG
2552 CCCGG
BpuAI 3094 GAAGAC GAAGAC(2/6)
2792 GTCTTC
BsaAI 2011 TACGTG [CT]AC^GT[GA]
2668 CACGTG
BsiHKAI 981 GAGCTC G[AT]GC[AT]^C
2462 GTGCAC
BspMI 231 GCAGGT ACCTGC(4/8)
1671 GCAGGT
BsrDI 2177 GCAATG GCAATG(2/0)
2252 GCAATG
BstEII 1807 GGTGACC G^GT[ACGT]ACC
2749 GGTGACC
BstPI 1807 GGTGACC G^GT[ACGT]ACC
2749 GGTGACC
EcoO65I 1807 GGTGACC G^GT[ACGT]ACC
2749 GGTGACC
EcoRV 2425 GATATC GAT^ATC
2524 GATATC
NciI 2036 CCCGG CC^[GC]GG
2552 CCCGG
NdeI 2739 CATATG CA^TATG
2941 CATATG
NspI 2139 ACATGC [GA]CATG^[CT]
2588 ACATGT
PpuMI 1167 AGGTCCC [GA]G^G[AT]CC[CT]
2927 AGGACCT
Psp1406I 747 AACGTT AA^CGTT
1292 AACGTT
XcmI 944 CCAGTTCTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1973 CCACAGCTTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BfaI BglII
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII