Restriction map for
pF1KB3031
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 8 CGCG CG^CG
AcyI 1141 GGCGTC G[GA]^CG[CT]C
AhdI 484 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 523 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1767 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 484 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1049 CCCGAG C^[CT]CG[GA]G
AviII 452 TGCGCA TGC^GCA
BanII 1671 GAGCCC G[GA]GC[CT]^C
BbsI 1720 GAAGAC GAAGAC(2/6)
BclI 855 TGATCA T^GATCA
BglI 214 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 573 AGATCT A^GATCT
BpuAI 1720 GAAGAC GAAGAC(2/6)
BsaBI 880 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1141 GGCGTC G[GA]^CG[CT]C
BsiEI 1361 CGGTCG CG[GA][CT]^CG
BsmFI 1399 GGGAC GGGAC(10/14)
BsoBI 1049 CCCGAG C^[CT]CG[GA]G
BsrBI 1175 CCGCTC CCGCTC(-3/-3)
BsrFI 1359 GCCGGT [GA]^CCGG[CT]
BstUI 8 CGCG CG^CG
Cfr10I 1359 GCCGGT [GA]^CCGG[CT]
DsaI 1275 CCACGG C^C[GA][CT]GG
Eam1105I 484 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1591 GAAGAG CTCTTC(1/4)
FauI 1680 CCCGC CCCGC(4/6)
FbaI 855 TGATCA T^GATCA
FspI 452 TGCGCA TGC^GCA
HaeII 1171 AGCGCC [GA]GCGC^[CT]
HgaI 1142 GCGTC GACGC(5/10)
Hin1I 1141 GGCGTC G[GA]^CG[CT]C
Ksp632I 1591 GAAGAG CTCTTC(1/4)
MamI 880 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 8 CGCG CG^CG
PstI 1374 CTGCAG CTGCA^G
SapI 1591 GAAGAGC GCTCTTC(1/4)
ScaI 624 AGTACT AGT^ACT
SmlI 634 CTCAAG C^T[CT][GA]AG
SspI 1630 AATATT AAT^ATT
StuI 94 AGGCCT AGG^CCT
TaqI 690 TCGA T^CGA
TthHB8I 690 TCGA T^CGA
XcmI 420 CCAGAGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1767 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1324 GTGCAC G^TGCAC
1369 GTGCAC
AlwI 316 GATCC GGATC(4/5)
886 GATCC
ApaLI 1324 GTGCAC G^TGCAC
1369 GTGCAC
AspHI 1324 GTGCAC G[AT]GC[AT]^C
1369 GTGCAC
BsaI 712 GAGACC GGTCTC(1/5)
1493 GGTCTC
BsgI 311 GTGCAG GTGCAG(16/14)
1706 GTGCAG
BsiHKAI 1324 GTGCAC G[AT]GC[AT]^C
1369 GTGCAC
BspHI 1061 TCATGA T^CATGA
1179 TCATGA
BsrDI 981 CATTGC GCAATG(2/0)
479 GCAATG
BstXI 1478 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1653 CCAGACGAGTGG
BstYI 315 AGATCC [GA]^GATC[CT]
573 AGATCT
DraIII 1276 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1700 CACCCTGTG
EaeI 1267 TGGCCG [CT]^GGCC[GA]
1389 TGGCCG
EcoNI 323 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1314 CCTGGAGGAGG
HincII 60 GTTGAC GT[CT]^[GA]AC
1450 GTTGAC
HindII 60 GTTGAC GT[CT]^[GA]AC
1450 GTTGAC
MflI 315 AGATCC [GA]^GATC[CT]
573 AGATCT
PleI 654 GACTC GAGTC(4/5)
1733 GAGTC
RcaI 1061 TCATGA T^CATGA
1179 TCATGA
Tsp45I 151 GTCAC ^GT[GC]AC
499 GTGAC
XhoII 315 AGATCC [GA]^GATC[CT]
573 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI