Restriction map for
pF1KB3013
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 428 CGCG CG^CG
AvaI 85 CCCGAG C^[CT]CG[GA]G
AvaII 950 GGTCC G^G[AT]CC
BalI 1097 TGGCCA TGG^CCA
BbsI 113 GAAGAC GAAGAC(2/6)
BpuAI 113 GAAGAC GAAGAC(2/6)
BseRI 1345 GAGGAG GAGGAG(10/8)
BsiYI 272 CCAAAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 272 CCAAAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 85 CCCGAG C^[CT]CG[GA]G
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrI 1280 ACTGG ACTGG(1/-1)
Bst1107I 764 GTATAC GTA^TAC
BstBI 599 TTCGAA TT^CGAA
BstEII 390 GGTTACC G^GT[ACGT]ACC
BstPI 390 GGTTACC G^GT[ACGT]ACC
BstUI 428 CGCG CG^CG
BstZ17I 764 GTATAC GTA^TAC
Cfr13I 950 GGTCC G^G[ACGT]CC
DraI 915 TTTAAA TTT^AAA
DsaI 65 CCACGG C^C[GA][CT]GG
EaeI 1097 TGGCCA [CT]^GGCC[GA]
EarI 801 GAAGAG CTCTTC(1/4)
EcoO65I 390 GGTTACC G^GT[ACGT]ACC
FauI 21 GCGGG CCCGC(4/6)
HaeIII 1098 GGCC GG^CC
HindIII 919 AAGCTT A^AGCTT
Ksp632I 801 GAAGAG CTCTTC(1/4)
MluNI 1097 TGGCCA TGG^CCA
MscI 1097 TGGCCA TGG^CCA
MspA1I 694 CAGCTG C[AC]G^C[GT]G
MvnI 428 CGCG CG^CG
NheI 99 GCTAGC G^CTAGC
NlaIII 706 CATG CATG^
NspV 599 TTCGAA TT^CGAA
PvuII 694 CAGCTG CAG^CTG
Sau96I 950 GGTCC G^G[ACGT]CC
ScaI 674 AGTACT AGT^ACT
SfuI 599 TTCGAA TT^CGAA
SmlI 997 CTTGAG C^T[CT][GA]AG
TfiI 690 GATTC G^A[AT]TC
Tsp45I 875 GTGAC ^GT[GC]AC
TspRI 319 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 764 GTATAC GT^[AC][GT]AC
1216 GTAGAC
AseI 369 ATTAAT AT^TAAT
1302 ATTAAT
AsnI 369 ATTAAT AT^TAAT
1302 ATTAAT
BamHI 712 GGATCC G^GATCC
1257 GGATCC
BbvI 484 GCAGC GCAGC(8/12)
789 GCAGC
BsaI 1248 GAGACC GGTCTC(1/5)
1283 GGTCTC
BssKI 642 CCAGG ^CC[ACGT]GG
825 CCTGG
BstNI 642 CCAGG CC^[AT]GG
825 CCTGG
DdeI 198 CTTAG C^T[ACGT]AG
1286 CTCAG
EcoRII 642 CCAGG ^CC[AT]GG
825 CCTGG
EcoT14I 104 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
HaeII 61 AGCGCC [GA]GCGC^[CT]
77 AGCGCC
HincII 1192 GTTAAC GT[CT]^[GA]AC
1315 GTTAAC
HindII 1192 GTTAAC GT[CT]^[GA]AC
1315 GTTAAC
HpaI 1192 GTTAAC GTT^AAC
1315 GTTAAC
MvaI 642 CCAGG CC^[AT]GG
825 CCTGG
MwoI 94 GCGAAGCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
564 GCAAATGAGGC
PshBI 369 ATTAAT AT^TAAT
1302 ATTAAT
ScrFI 642 CCAGG CC^[ACGT]GG
825 CCTGG
SspI 256 AATATT AAT^ATT
1084 AATATT
StyI 104 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
TseI 484 GCAGC G^C[AT]GC
789 GCAGC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BfaI BsaJI
BsmAI BstF5I BstYI Cac8I CfoI
DpnII Fnu4HI FokI HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIV PleI RsaI Sau3AI
SfaNI SfcI TaqI Tru9I Tsp509I
TthHB8I XhoII XmnI