Restriction map for
pF1KB3004
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BcnI BfrI
BglI BlnI BsaAI BsaI BsiEI
BsiWI BspDI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
KpnI KspI MfeI MluI MluNI
MscI MunI MvnI NaeI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2851 GTAGAC GT^[AC][GT]AC
AccIII 1259 TCCGGA T^CCGGA
AcyI 2349 GGCGCC G[GA]^CG[CT]C
AocI 2751 CCTTAGG CC^T[ACGT]AGG
AvaI 593 CTCGAG C^[CT]CG[GA]G
BbeI 2349 GGCGCC GGCGC^C
BbvCI 2778 CCTCAGC CCTCAGC(-5/-2)
BciVI 1852 GTATCC GTATCC(6/5)
BglII 1926 AGATCT A^GATCT
BlpI 2004 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2004 GCTGAGC GC^T[ACGT]AGC
BsaBI 2452 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2349 GGCGCC G[GA]^CG[CT]C
BseAI 1259 TCCGGA T^CCGGA
BsmBI 1408 GAGACG CGTCTC(1/5)
BsmI 3003 GAATGC GAATGC(1/-1)
BsoBI 593 CTCGAG C^[CT]CG[GA]G
BspEI 1259 TCCGGA T^CCGGA
BspHI 1866 TCATGA T^CATGA
BspMI 947 GCAGGT ACCTGC(4/8)
BsrDI 395 GCAATG GCAATG(2/0)
Bsu36I 2751 CCTTAGG CC^T[ACGT]AGG
CelII 2004 GCTGAGC GC^T[ACGT]AGC
CfoI 2350 GCGC GCG^C
DraI 2442 TTTAAA TTT^AAA
Eco81I 2751 CCTTAGG CC^T[ACGT]AGG
EcoRI 1225 GAATTC G^AATTC
FauI 1000 CCCGC CCCGC(4/6)
HaeII 2349 GGCGCC [GA]GCGC^[CT]
HgaI 1162 GCGTC GACGC(5/10)
HhaI 2350 GCGC GCG^C
Hin1I 2349 GGCGCC G[GA]^CG[CT]C
HinP1I 2350 GCGC G^CGC
HindIII 2714 AAGCTT A^AGCTT
HpaI 3120 GTTAAC GTT^AAC
KasI 2349 GGCGCC G^GCGCC
MamI 2452 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1259 TCCGGA T^CCGGA
MslI 2159 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2349 GGCGCC GG^CGCC
NdeI 1025 CATATG CA^TATG
PaeR7I 593 CTCGAG C^TCGAG
RcaI 1866 TCATGA T^CATGA
SacI 716 GAGCTC GAGCT^C
SexAI 2846 ACCAGGT A^CC[AT]GGT
SfoI 2349 GGCGCC GGC^GCC
SpeI 3124 ACTAGT A^CTAGT
SspI 2947 AATATT AAT^ATT
StuI 964 AGGCCT AGG^CCT
XbaI 1929 TCTAGA T^CTAGA
XhoI 593 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AclI 297 AACGTT AA^CGTT
2438 AACGTT
BanI 2349 GGCGCC G^G[CT][GA]CC
2504 GGCACC
BanII 716 GAGCTC G[GA]GC[CT]^C
2007 GAGCCC
BclI 1395 TGATCA T^GATCA
2457 TGATCA
BpmI 1721 CTCCAG CTGGAG(16/14)
49 CTGGAG
BsaWI 1259 TCCGGA [AT]^CCGG[AT]
1917 ACCGGA
BsiYI 268 CCTCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
896 CCATGCCCAGG
BslI 268 CCTCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
896 CCATGCCCAGG
BssSI 1646 CACGAG CACGAG(-5/-1)
968 CTCGTG
BstXI 41 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
432 CCAAGGGATTGG
FbaI 1395 TGATCA T^GATCA
2457 TGATCA
HapII 1260 CCGG C^CGG
1918 CCGG
HpaII 1260 CCGG C^CGG
1918 CCGG
MaeII 298 ACGT A^CGT
2439 ACGT
MspI 1260 CCGG C^CGG
1918 CCGG
PshAI 563 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2644 GACTTGAGTC
Psp1406I 297 AACGTT AA^CGTT
2438 AACGTT
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI