Restriction map for
pF1KB3003
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BcgI BciVI BfrI
BglII BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Van91I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1199 GGTACC G^GTACC
AccII 319 CGCG CG^CG
Aor51HI 368 AGCGCT AGC^GCT
Asp718I 1199 GGTACC G^GTACC
AspI 376 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1119 CCTAGG C^CTAGG
BamHI 1770 GGATCC G^GATCC
BclI 1751 TGATCA T^GATCA
BlnI 1119 CCTAGG C^CTAGG
BlpI 191 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 191 GCTCAGC GC^T[ACGT]AGC
BsgI 59 GTGCAG GTGCAG(16/14)
BsmI 1813 GCATTC GAATGC(1/-1)
BsrDI 1844 GCAATG GCAATG(2/0)
BstEII 264 GGTCACC G^GT[ACGT]ACC
BstPI 264 GGTCACC G^GT[ACGT]ACC
BstUI 319 CGCG CG^CG
CelII 191 GCTCAGC GC^T[ACGT]AGC
DraIII 166 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1553 CCACGG C^C[GA][CT]GG
Eco47III 368 AGCGCT AGC^GCT
EcoNI 1190 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 264 GGTCACC G^GT[ACGT]ACC
FauI 99 GCGGG CCCGC(4/6)
FbaI 1751 TGATCA T^GATCA
HgaI 1176 GCGTC GACGC(5/10)
KpnI 1199 GGTACC GGTAC^C
MaeII 1694 ACGT A^CGT
MvnI 319 CGCG CG^CG
PaeR7I 1717 CTCGAG C^TCGAG
PflFI 376 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 33 CTGCAG CTGCA^G
SfaNI 1136 GCATC GCATC(5/9)
SfiI 1123 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1061 CCCGGG CCC^GGG
SphI 1172 GCATGC GCATG^C
TfiI 1450 GATTC G^A[AT]TC
Tth111I 376 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1518 CCAGGAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1717 CTCGAG C^TCGAG
XmaCI 1061 CCCGGG C^CCGGG
XmaI 1061 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 857 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1604 CAGTCCCTG
Asp700I 64 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
993 GAACCCCTTC
BalI 510 TGGCCA TGG^CCA
1515 TGGCCA
BbvCI 668 CCTCAGC CCTCAGC(-5/-2)
1473 GCTGAGG
BglI 296 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1124 GCCTGGGAGGC
BsaI 813 GAGACC GGTCTC(1/5)
1366 GAGACC
BsrBI 1002 CCGCTC CCGCTC(-3/-3)
1672 GAGCGG
BstXI 125 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1872 CCAGCTCTATGG
DrdI 247 GACTATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
340 GACAAGCTGGTC
EcoT14I 107 CCAAGG C^C[AT][AT]GG
1119 CCTAGG
HindIII 505 AAGCTT A^AGCTT
1279 AAGCTT
MluNI 510 TGGCCA TGG^CCA
1515 TGGCCA
MscI 510 TGGCCA TGG^CCA
1515 TGGCCA
NspI 326 GCATGT [GA]CATG^[CT]
1172 GCATGC
SacI 1612 GAGCTC GAGCT^C
1714 GAGCTC
StuI 1122 AGGCCT AGG^CCT
1241 AGGCCT
StyI 107 CCAAGG C^C[AT][AT]GG
1119 CCTAGG
TaqI 49 TCGA T^CGA
1718 TCGA
Tsp509I 387 AATT ^AATT
821 AATT
TthHB8I 49 TCGA T^CGA
1718 TCGA
XmnI 64 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
993 GAACCCCTTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI TseI Tsp45I TspRI XhoII