Restriction map for
pF1KB2048
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BalI BbrPI BciVI BclI
BfrI BglI BlnI BsaBI BsiWI
BspDI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoT14I EcoT22I FbaI FseI
FspI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SpeI SphI StuI StyI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 2831 GGTACC G^GTACC
AccI 2821 GTCTAC GT^[AC][GT]AC
AccIII 380 TCCGGA T^CCGGA
AflIII 940 ACATGT A^C[GA][CT]GT
AocI 3039 CCTGAGG CC^T[ACGT]AGG
Asp700I 169 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2831 GGTACC G^GTACC
AspI 1662 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2210 GGATCC G^GATCC
BbeI 1537 GGCGCC GGCGC^C
BbsI 774 GAAGAC GAAGAC(2/6)
BbvCI 1803 GCTGAGG CCTCAGC(-5/-2)
BglII 2477 AGATCT A^GATCT
BpuAI 774 GAAGAC GAAGAC(2/6)
BsaAI 300 TACGTA [CT]AC^GT[GA]
BseAI 380 TCCGGA T^CCGGA
BsiEI 952 CGGCCG CG[GA][CT]^CG
Bsp1407I 2105 TGTACA T^GTACA
BspEI 380 TCCGGA T^CCGGA
BspHI 1154 TCATGA T^CATGA
BspLU11I 940 ACATGT A^CATGT
BspMI 1584 ACCTGC ACCTGC(4/8)
BsrDI 2341 CATTGC GCAATG(2/0)
BsrGI 2105 TGTACA T^GTACA
BssSI 2797 CACGAG CACGAG(-5/-1)
BstEII 689 GGTCACC G^GT[ACGT]ACC
BstPI 689 GGTCACC G^GT[ACGT]ACC
BstXI 1259 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3039 CCTGAGG CC^T[ACGT]AGG
DsaI 1424 CCACGG C^C[GA][CT]GG
EagI 952 CGGCCG C^GGCCG
EclXI 952 CGGCCG C^GGCCG
Eco52I 952 CGGCCG C^GGCCG
Eco81I 3039 CCTGAGG CC^T[ACGT]AGG
EcoO65I 689 GGTCACC G^GT[ACGT]ACC
EcoRV 1318 GATATC GAT^ATC
HindIII 340 AAGCTT A^AGCTT
HpaI 1656 GTTAAC GTT^AAC
KasI 1537 GGCGCC G^GCGCC
KpnI 2831 GGTACC GGTAC^C
MroI 380 TCCGGA T^CCGGA
NarI 1537 GGCGCC GG^CGCC
NheI 435 GCTAGC G^CTAGC
PflFI 1662 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 545 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1154 TCATGA T^CATGA
SbfI 1585 CCTGCAGG CCTGCA^GG
SfoI 1537 GGCGCC GGC^GCC
SnaBI 300 TACGTA TAC^GTA
Sse8387I 1585 CCTGCAGG CCTGCA^GG
SspBI 2105 TGTACA T^GTACA
SspI 172 AATATT AAT^ATT
Tth111I 1662 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 169 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 494 AAATTC [GA]^AATT[CT]
823 AAATTC
AcyI 1537 GGCGCC G[GA]^CG[CT]C
2632 GACGCC
ApoI 494 AAATTC [GA]^AATT[CT]
823 AAATTC
AspHI 1733 GTGCTC G[AT]GC[AT]^C
2643 GTGCTC
BanII 1269 GGGCTC G[GA]GC[CT]^C
1455 GGGCTC
BcgI 2866 CGAGAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
317 GCACCGTGTTCG
BsaHI 1537 GGCGCC G[GA]^CG[CT]C
2632 GACGCC
BsiHKAI 1733 GTGCTC G[AT]GC[AT]^C
2643 GTGCTC
BsrBI 918 CCGCTC CCGCTC(-3/-3)
1711 GAGCGG
BstYI 2210 GGATCC [GA]^GATC[CT]
2477 AGATCT
DraIII 1727 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3012 CACCGAGTG
EaeI 952 CGGCCG [CT]^GGCC[GA]
1368 TGGCCG
EcoNI 144 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1095 CCTGAGCGAGG
Hin1I 1537 GGCGCC G[GA]^CG[CT]C
2632 GACGCC
MflI 2210 GGATCC [GA]^GATC[CT]
2477 AGATCT
MslI 1956 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2109 CATTGAGGTG
NspI 211 GCATGT [GA]CATG^[CT]
940 ACATGT
PshAI 18 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1945 GACTCTCGTC
SapI 1110 GAAGAGC GCTCTTC(1/4)
2706 GCTCTTC
XhoII 2210 GGATCC [GA]^GATC[CT]
2477 AGATCT
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI