Restriction map for
pF1KB1499
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# NO CUT ENZYMES ( 62 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI AscI Asp700I AspEI BcgI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BspHI BssSI Bst1107I BstZ17I
ClaI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
HindIII HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Aor51HI 1046 AGCGCT AGC^GCT
AseI 3403 ATTAAT AT^TAAT
AsnI 3403 ATTAAT AT^TAAT
AviII 995 TGCGCA TGC^GCA
BamHI 2539 GGATCC G^GATCC
BbeI 353 GGCGCC GGCGC^C
BbrPI 3426 CACGTG CAC^GTG
BclI 3102 TGATCA T^GATCA
BglII 1884 AGATCT A^GATCT
BsiEI 687 CGACCG CG[GA][CT]^CG
BsmBI 547 CGTCTC CGTCTC(1/5)
Bsp1407I 2354 TGTACA T^GTACA
BspLU11I 3064 ACATGT A^CATGT
BspMI 3004 GCAGGT ACCTGC(4/8)
BsrBI 3354 CCGCTC CCGCTC(-3/-3)
BsrGI 2354 TGTACA T^GTACA
BssHII 824 GCGCGC G^CGCGC
BstBI 2905 TTCGAA TT^CGAA
BstEII 1197 GGTCACC G^GT[ACGT]ACC
BstPI 1197 GGTCACC G^GT[ACGT]ACC
CpoI 1021 CGGACCG CG^G[AT]CCG
EarI 1189 GAAGAG CTCTTC(1/4)
Eco47III 1046 AGCGCT AGC^GCT
EcoO65I 1197 GGTCACC G^GT[ACGT]ACC
EcoRV 82 GATATC GAT^ATC
FbaI 3102 TGATCA T^GATCA
FspI 995 TGCGCA TGC^GCA
HincII 2146 GTCAAC GT[CT]^[GA]AC
HindII 2146 GTCAAC GT[CT]^[GA]AC
KasI 353 GGCGCC G^GCGCC
Ksp632I 1189 GAAGAG CTCTTC(1/4)
KspI 3267 CCGCGG CCGC^GG
MseI 3404 TTAA T^TAA
NarI 353 GGCGCC GG^CGCC
NspV 2905 TTCGAA TT^CGAA
PmaCI 3426 CACGTG CAC^GTG
PmlI 3426 CACGTG CAC^GTG
PshBI 3403 ATTAAT AT^TAAT
PvuII 874 CAGCTG CAG^CTG
RsrII 1021 CGGACCG CG^G[AT]CCG
SacII 3267 CCGCGG CCGC^GG
SexAI 452 ACCTGGT A^CC[AT]GGT
SfiI 531 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 353 GGCGCC GGC^GCC
SfuI 2905 TTCGAA TT^CGAA
SgrAI 964 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2354 TGTACA T^GTACA
StuI 539 AGGCCT AGG^CCT
Tru9I 3404 TTAA T^TAA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 1003 GTCTAC GT^[AC][GT]AC
2661 GTCTAC
AflIII 3064 ACATGT A^C[GA][CT]GT
3425 ACACGT
Alw44I 830 GTGCAC G^TGCAC
3019 GTGCAC
ApaLI 830 GTGCAC G^TGCAC
3019 GTGCAC
AspI 246 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
292 GACAATGTC
AvrII 1429 CCTAGG C^CTAGG
2766 CCTAGG
BbvCI 1284 CCTCAGC CCTCAGC(-5/-2)
526 GCTGAGG
BlnI 1429 CCTAGG C^CTAGG
2766 CCTAGG
BsaAI 790 TACGTG [CT]AC^GT[GA]
3426 CACGTG
BstAPI 42 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1378 GCAGGCACTGC
DraIII 1976 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3751 CACCATGTG
EcoNI 1179 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2625 CCTGGAGCAGG
NspI 113 ACATGC [GA]CATG^[CT]
3064 ACATGT
PflFI 246 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
292 GACAATGTC
PshAI 326 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3369 GACCAGCGTC
SacI 1070 GAGCTC GAGCT^C
1677 GAGCTC
SmlI 2574 CTTGAG C^T[CT][GA]AG
2676 CTCAAG
Tth111I 246 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
292 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI