Restriction map for
pF1KB1393
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsiEI
BsiWI BsmBI BsoBI BspDI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MflI MluI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 2353 GTCTAC GT^[AC][GT]AC
AccIII 2160 TCCGGA T^CCGGA
AlwNI 253 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 178 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2329 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1557 TGGCCA TGG^CCA
BbeI 2123 GGCGCC GGCGC^C
BbrPI 1864 CACGTG CAC^GTG
BbsI 934 GAAGAC GAAGAC(2/6)
BglI 472 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 261 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 261 GCTAAGC GC^T[ACGT]AGC
BpuAI 934 GAAGAC GAAGAC(2/6)
BsaAI 1864 CACGTG [CT]AC^GT[GA]
BseAI 2160 TCCGGA T^CCGGA
BsmI 1778 GAATGC GAATGC(1/-1)
Bsp1407I 1947 TGTACA T^GTACA
BspEI 2160 TCCGGA T^CCGGA
BspHI 1755 TCATGA T^CATGA
BspMI 138 GCAGGT ACCTGC(4/8)
BsrDI 878 CATTGC GCAATG(2/0)
BsrFI 1904 ACCGGC [GA]^CCGG[CT]
BsrGI 1947 TGTACA T^GTACA
BssSI 33 CTCGTG CACGAG(-5/-1)
BstAPI 1999 GCAGAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 261 GCTAAGC GC^T[ACGT]AGC
CfoI 2124 GCGC GCG^C
Cfr10I 1904 ACCGGC [GA]^CCGG[CT]
DraI 826 TTTAAA TTT^AAA
DrdI 303 GACTGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 2123 GGCGCC [GA]GCGC^[CT]
HhaI 2124 GCGC GCG^C
HinP1I 2124 GCGC G^CGC
KasI 2123 GGCGCC G^GCGCC
MluNI 1557 TGGCCA TGG^CCA
MroI 2160 TCCGGA T^CCGGA
MscI 1557 TGGCCA TGG^CCA
NarI 2123 GGCGCC GG^CGCC
NcoI 2034 CCATGG C^CATGG
NspI 1872 GCATGT [GA]CATG^[CT]
PflFI 2329 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1538 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1864 CACGTG CAC^GTG
PmlI 1864 CACGTG CAC^GTG
RcaI 1755 TCATGA T^CATGA
SacI 400 GAGCTC GAGCT^C
SfoI 2123 GGCGCC GGC^GCC
SgrAI 1903 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1947 TGTACA T^GTACA
StuI 2040 AGGCCT AGG^CCT
Tth111I 2329 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1538 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 178 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 602 AAATTC [GA]^AATT[CT]
1341 AAATTT
AocI 1582 CCTCAGG CC^T[ACGT]AGG
2118 CCTCAGG
ApoI 602 AAATTC [GA]^AATT[CT]
1341 AAATTT
BanI 407 GGCACC G^G[CT][GA]CC
2123 GGCGCC
BbvCI 2373 CCTCAGC CCTCAGC(-5/-2)
1134 GCTGAGG
BpmI 1501 CTGGAG CTGGAG(16/14)
2053 CTGGAG
Bsu36I 1582 CCTCAGG CC^T[ACGT]AGG
2118 CCTCAGG
DraIII 214 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1963 CACAGGGTG
DsaI 1118 CCACGG C^C[GA][CT]GG
2034 CCATGG
Eco81I 1582 CCTCAGG CC^T[ACGT]AGG
2118 CCTCAGG
HincII 1768 GTTGAC GT[CT]^[GA]AC
1876 GTCAAC
HindII 1768 GTTGAC GT[CT]^[GA]AC
1876 GTCAAC
HindIII 1051 AAGCTT A^AGCTT
1633 AAGCTT
MspA1I 493 CAGCTG C[AC]G^C[GT]G
1913 CAGCTG
PvuII 493 CAGCTG CAG^CTG
1913 CAGCTG
SexAI 215 ACCAGGT A^CC[AT]GGT
2411 ACCAGGT
SfcI 860 CTACAG C^T[GA][CT]AG
2355 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI