Restriction map for
pF1KB1325
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BfaI BfrI BlnI BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstUI Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoNI
EcoRI EcoT22I FseI FspI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 1186 GACGCC G[GA]^CG[CT]C
AlwNI 365 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1175 AGCGCT AGC^GCT
ApaI 1293 GGGCCC GGGCC^C
BcgI 1751 GCATAGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1465 GGATAC GTATCC(6/5)
BclI 1794 TGATCA T^GATCA
BlpI 2023 GCTGAGC GC^T[ACGT]AGC
BpmI 1357 CTGGAG CTGGAG(16/14)
Bpu1102I 2023 GCTGAGC GC^T[ACGT]AGC
BsaHI 1186 GACGCC G[GA]^CG[CT]C
BsaWI 787 ACCGGA [AT]^CCGG[AT]
BsgI 131 CTGCAC GTGCAG(16/14)
BsiEI 1688 CGGCCG CG[GA][CT]^CG
BsmBI 883 GAGACG CGTCTC(1/5)
BsrFI 1923 GCCGGT [GA]^CCGG[CT]
Bst1107I 490 GTATAC GTA^TAC
BstEII 630 GGTCACC G^GT[ACGT]ACC
BstPI 630 GGTCACC G^GT[ACGT]ACC
BstXI 1490 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 490 GTATAC GTA^TAC
CelII 2023 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1923 GCCGGT [GA]^CCGG[CT]
DraIII 946 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1688 CGGCCG C^GGCCG
EarI 2065 CTCTTC CTCTTC(1/4)
EclXI 1688 CGGCCG C^GGCCG
Eco47III 1175 AGCGCT AGC^GCT
Eco52I 1688 CGGCCG C^GGCCG
EcoO65I 630 GGTCACC G^GT[ACGT]ACC
EcoRV 791 GATATC GAT^ATC
FbaI 1794 TGATCA T^GATCA
Hin1I 1186 GACGCC G[GA]^CG[CT]C
HincII 6 GTCGAC GT[CT]^[GA]AC
HindII 6 GTCGAC GT[CT]^[GA]AC
HindIII 257 AAGCTT A^AGCTT
Ksp632I 2065 CTCTTC CTCTTC(1/4)
PaeR7I 741 CTCGAG C^TCGAG
PflMI 986 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 811 CAGCTG CAG^CTG
SalI 6 GTCGAC G^TCGAC
SapI 2064 GCTCTTC GCTCTTC(1/4)
Tsp509I 2185 AATT ^AATT
Van91I 986 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 741 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 6 GTCGAC GT^[AC][GT]AC
490 GTATAC
AvaI 568 CTCGGG C^[CT]CG[GA]G
741 CTCGAG
BamHI 1485 GGATCC G^GATCC
1694 GGATCC
BanI 978 GGCACC G^G[CT][GA]CC
1704 GGCACC
BbsI 1227 GAAGAC GAAGAC(2/6)
1235 GTCTTC
BglII 869 AGATCT A^GATCT
1082 AGATCT
BpuAI 1227 GAAGAC GAAGAC(2/6)
1235 GTCTTC
BsaI 514 GAGACC GGTCTC(1/5)
1972 GGTCTC
BsoBI 568 CTCGGG C^[CT]CG[GA]G
741 CTCGAG
BspMI 87 GCAGGT ACCTGC(4/8)
624 GCAGGT
HaeII 920 AGCGCC [GA]GCGC^[CT]
1175 AGCGCT
MaeII 10 ACGT A^CGT
704 ACGT
MspA1I 811 CAGCTG C[AC]G^C[GT]G
2211 CCGCTG
PleI 49 GACTC GAGTC(4/5)
1779 GACTC
SexAI 41 ACCTGGT A^CC[AT]GGT
1142 ACCTGGT
TfiI 682 GATTC G^A[AT]TC
1039 GAATC
XcmI 539 CCAAACCTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1472 CCAACTTCATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbvI BcnI
BglI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PpuMI
PstI RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I XhoII