Restriction map for
pF1KB1123
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# NO CUT ENZYMES ( 43 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AvrII BbrPI BfrI BlnI
BsiWI BssHII Bst1107I BstZ17I CpoI
DraI FseI HindIII KspI MfeI
MluI MunI NaeI NgoMIV NotI
NruI PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SnaBI
Sse8387I SspI SwaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 5861 GACGTC GACGT^C
Acc65I 5897 GGTACC G^GTACC
AccIII 6816 TCCGGA T^CCGGA
AhdI 3523 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 7429 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5897 GGTACC G^GTACC
AspEI 3523 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2189 TGCGCA TGC^GCA
BbeI 3310 GGCGCC GGCGC^C
BsaAI 430 TACGTG [CT]AC^GT[GA]
BseAI 6816 TCCGGA T^CCGGA
BspDI 5131 ATCGAT AT^CGAT
BspEI 6816 TCCGGA T^CCGGA
BsrBI 1379 GAGCGG CCGCTC(-3/-3)
BsrFI 1076 GCCGGT [GA]^CCGG[CT]
BstEII 5085 GGTGACC G^GT[ACGT]ACC
BstPI 5085 GGTGACC G^GT[ACGT]ACC
Cfr10I 1076 GCCGGT [GA]^CCGG[CT]
ClaI 5131 ATCGAT AT^CGAT
Eam1105I 3523 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5085 GGTGACC G^GT[ACGT]ACC
EcoRV 1498 GATATC GAT^ATC
FspI 2189 TGCGCA TGC^GCA
HpaI 6613 GTTAAC GTT^AAC
KasI 3310 GGCGCC G^GCGCC
KpnI 5897 GGTACC GGTAC^C
MroI 6816 TCCGGA T^CCGGA
NarI 3310 GGCGCC GG^CGCC
NheI 271 GCTAGC G^CTAGC
PshAI 5887 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 3310 GGCGCC GGC^GCC
SmaI 2726 CCCGGG CCC^GGG
SpeI 2791 ACTAGT A^CTAGT
XbaI 4236 TCTAGA T^CTAGA
XmaCI 2726 CCCGGG C^CCGGG
XmaI 2726 CCCGGG C^CCGGG
XmnI 7429 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 44 enzymes) #
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AccI 177 GTCTAC GT^[AC][GT]AC
5943 GTCTAC
AccII 1360 CGCG CG^CG
5431 CGCG
AflIII 5108 ACATGT A^C[GA][CT]GT
5990 ACATGT
AocI 1720 CCTGAGG CC^T[ACGT]AGG
5080 CCTCAGG
AseI 2334 ATTAAT AT^TAAT
4255 ATTAAT
AsnI 2334 ATTAAT AT^TAAT
4255 ATTAAT
AspI 3426 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7234 GACAGTGTC
BcgI 674 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1970 GCAAACTGCTCG
BciVI 3655 GGATAC GTATCC(6/5)
5703 GGATAC
BclI 1110 TGATCA T^GATCA
2381 TGATCA
BlpI 1965 GCTTAGC GC^T[ACGT]AGC
3858 GCTGAGC
Bpu1102I 1965 GCTTAGC GC^T[ACGT]AGC
3858 GCTGAGC
BsaBI 2196 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2865 GATGCGGATC
BsaWI 10 ACCGGA [AT]^CCGG[AT]
6816 TCCGGA
Bsp1407I 963 TGTACA T^GTACA
5636 TGTACA
BspLU11I 5108 ACATGT A^CATGT
5990 ACATGT
BsrGI 963 TGTACA T^GTACA
5636 TGTACA
BssSI 4360 CACGAG CACGAG(-5/-1)
5684 CACGAG
BstBI 3479 TTCGAA TT^CGAA
5392 TTCGAA
BstUI 1360 CGCG CG^CG
5431 CGCG
Bsu36I 1720 CCTGAGG CC^T[ACGT]AGG
5080 CCTCAGG
CelII 1965 GCTTAGC GC^T[ACGT]AGC
3858 GCTGAGC
DraIII 2695 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
7201 CACACAGTG
DrdI 355 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACGCCACAGTC
EagI 4286 CGGCCG C^GGCCG
5501 CGGCCG
EclXI 4286 CGGCCG C^GGCCG
5501 CGGCCG
Eco52I 4286 CGGCCG C^GGCCG
5501 CGGCCG
Eco81I 1720 CCTGAGG CC^T[ACGT]AGG
5080 CCTCAGG
EcoRI 3118 GAATTC G^AATTC
4084 GAATTC
EcoT22I 3247 ATGCAT ATGCA^T
3728 ATGCAT
FbaI 1110 TGATCA T^GATCA
2381 TGATCA
MamI 2196 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2865 GATGCGGATC
MvnI 1360 CGCG CG^CG
5431 CGCG
NsiI 3247 ATGCAT ATGCA^T
3728 ATGCAT
NspV 3479 TTCGAA TT^CGAA
5392 TTCGAA
PaeR7I 1469 CTCGAG C^TCGAG
4450 CTCGAG
PflFI 3426 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7234 GACAGTGTC
PshBI 2334 ATTAAT AT^TAAT
4255 ATTAAT
SapI 3069 GAAGAGC GCTCTTC(1/4)
6600 GCTCTTC
ScaI 893 AGTACT AGT^ACT
5321 AGTACT
SfuI 3479 TTCGAA TT^CGAA
5392 TTCGAA
SspBI 963 TGTACA T^GTACA
5636 TGTACA
Tth111I 3426 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7234 GACAGTGTC
XhoI 1469 CTCGAG C^TCGAG
4450 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII