Restriction map for
pF1KB0668
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspI AvaI AviII AvrII BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsoBI BspDI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HindIII HpaI KspI MamI MluI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 732 GGTACC G^GTACC
AccIII 918 TCCGGA T^CCGGA
AgeI 693 ACCGGT A^CCGGT
AhdI 1771 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 452 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 732 GGTACC G^GTACC
AspEI 1771 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2714 TGGCCA TGG^CCA
BamHI 373 GGATCC G^GATCC
BanII 749 GGGCTC G[GA]GC[CT]^C
BcnI 157 CCGGG CC^[GC]GG
BpmI 677 CTCCAG CTGGAG(16/14)
BsaI 2369 GGTCTC GGTCTC(1/5)
BseAI 918 TCCGGA T^CCGGA
BsgI 709 GTGCAG GTGCAG(16/14)
BsmBI 1020 GAGACG CGTCTC(1/5)
BsmI 390 GAATGC GAATGC(1/-1)
Bsp1407I 2032 TGTACA T^GTACA
BspEI 918 TCCGGA T^CCGGA
BsrDI 585 CATTGC GCAATG(2/0)
BsrGI 2032 TGTACA T^GTACA
BstBI 411 TTCGAA TT^CGAA
DraII 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 83 CCACGG C^C[GA][CT]GG
Eam1105I 1771 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 521 GAATTC G^AATTC
EcoT22I 392 ATGCAT ATGCA^T
HincII 1327 GTTGAC GT[CT]^[GA]AC
HindII 1327 GTTGAC GT[CT]^[GA]AC
KpnI 732 GGTACC GGTAC^C
MluNI 2714 TGGCCA TGG^CCA
MroI 918 TCCGGA T^CCGGA
MscI 2714 TGGCCA TGG^CCA
NciI 157 CCGGG CC^[GC]GG
NsiI 392 ATGCAT ATGCA^T
NspI 1686 GCATGC [GA]CATG^[CT]
NspV 411 TTCGAA TT^CGAA
PinAI 693 ACCGGT A^CCGGT
PvuII 598 CAGCTG CAG^CTG
SexAI 423 ACCTGGT A^CC[AT]GGT
SfuI 411 TTCGAA TT^CGAA
SmlI 1322 CTCAAG C^T[CT][GA]AG
SphI 1686 GCATGC GCATG^C
SspBI 2032 TGTACA T^GTACA
StuI 125 AGGCCT AGG^CCT
XmnI 452 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 428 GTGCTC G[AT]GC[AT]^C
2669 GTGCTC
BbeI 34 GGCGCC GGCGC^C
2302 GGCGCC
BciVI 535 GGATAC GTATCC(6/5)
2060 GGATAC
BfaI 1096 CTAG C^TAG
1609 CTAG
BsaWI 693 ACCGGT [AT]^CCGG[AT]
918 TCCGGA
BsiHKAI 428 GTGCTC G[AT]GC[AT]^C
2669 GTGCTC
BspMI 735 ACCTGC ACCTGC(4/8)
729 GCAGGT
BsrFI 693 ACCGGT [GA]^CCGG[CT]
717 ACCGGC
Cfr10I 693 ACCGGT [GA]^CCGG[CT]
717 ACCGGC
KasI 34 GGCGCC G^GCGCC
2302 GGCGCC
MaeI 1096 CTAG C^TAG
1609 CTAG
MaeII 2677 ACGT A^CGT
2849 ACGT
MslI 1530 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1681 CATCTGCATG
MspA1I 27 CAGCGG C[AC]G^C[GT]G
598 CAGCTG
NarI 34 GGCGCC GG^CGCC
2302 GGCGCC
PflMI 640 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1567 CCAAGTATTGG
PstI 308 CTGCAG CTGCA^G
601 CTGCAG
SapI 1879 GAAGAGC GCTCTTC(1/4)
2896 GCTCTTC
SfcI 308 CTGCAG C^T[GA][CT]AG
601 CTGCAG
SfoI 34 GGCGCC GGC^GCC
2302 GGCGCC
Van91I 640 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1567 CCAAGTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BanI BbsI
BbvI BmyI BpuAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MfeI MflI
MnlI MseI MspI MunI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII