Restriction map for
pF1KB0174
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccII AflII AscI AseI AsnI
AspI AviII BalI BbeI BbrPI
BfrI BglI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstUI
BstZ17I ClaI CpoI DraIII DrdI
EaeI EagI EclXI Eco52I FseI
FspI HindIII HpaI KasI KspI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 1706 GACGTC GACGT^C
Acc65I 3487 GGTACC G^GTACC
AccIII 1377 TCCGGA T^CCGGA
AgeI 2763 ACCGGT A^CCGGT
AhdI 3373 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3683 GTGCAC G^TGCAC
Aor51HI 374 AGCGCT AGC^GCT
ApaI 3957 GGGCCC GGGCC^C
ApaLI 3683 GTGCAC G^TGCAC
Asp718I 3487 GGTACC G^GTACC
AspEI 3373 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 122 CTCGAG C^[CT]CG[GA]G
AvrII 1291 CCTAGG C^CTAGG
BbvCI 1879 GCTGAGG CCTCAGC(-5/-2)
BcgI 2676 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2009 GGATAC GTATCC(6/5)
BclI 2637 TGATCA T^GATCA
BcnI 537 CCCGG CC^[GC]GG
BglII 3097 AGATCT A^GATCT
BlnI 1291 CCTAGG C^CTAGG
BsaAI 1778 TACGTG [CT]AC^GT[GA]
BseAI 1377 TCCGGA T^CCGGA
BsgI 239 CTGCAC GTGCAG(16/14)
BsoBI 122 CTCGAG C^[CT]CG[GA]G
BspEI 1377 TCCGGA T^CCGGA
BspMI 730 GCAGGT ACCTGC(4/8)
BsrFI 2763 ACCGGT [GA]^CCGG[CT]
BstAPI 309 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2804 TTCGAA TT^CGAA
BstEII 465 GGTCACC G^GT[ACGT]ACC
BstPI 465 GGTCACC G^GT[ACGT]ACC
CfoI 375 GCGC GCG^C
Cfr10I 2763 ACCGGT [GA]^CCGG[CT]
Eam1105I 3373 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 374 AGCGCT AGC^GCT
EcoNI 3866 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 465 GGTCACC G^GT[ACGT]ACC
EcoRI 3263 GAATTC G^AATTC
EcoRV 1363 GATATC GAT^ATC
EcoT22I 3301 ATGCAT ATGCA^T
FauI 2853 GCGGG CCCGC(4/6)
FbaI 2637 TGATCA T^GATCA
HaeII 374 AGCGCT [GA]GCGC^[CT]
HhaI 375 GCGC GCG^C
HinP1I 375 GCGC G^CGC
KpnI 3487 GGTACC GGTAC^C
MroI 1377 TCCGGA T^CCGGA
NciI 537 CCCGG CC^[GC]GG
NsiI 3301 ATGCAT ATGCA^T
NspV 2804 TTCGAA TT^CGAA
PaeR7I 122 CTCGAG C^TCGAG
PinAI 2763 ACCGGT A^CCGGT
PshAI 2733 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1585 GAGCTC GAGCT^C
SfuI 2804 TTCGAA TT^CGAA
SpeI 3847 ACTAGT A^CTAGT
StuI 141 AGGCCT AGG^CCT
XhoI 122 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 2541 ACATGT A^C[GA][CT]GT
3028 ACATGT
AlwNI 391 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2305 CAGCTTCTG
AocI 1108 CCTAAGG CC^T[ACGT]AGG
3375 CCTTAGG
BamHI 174 GGATCC G^GATCC
2289 GGATCC
BanI 2020 GGTGCC G^G[CT][GA]CC
3487 GGTACC
BlpI 745 GCTGAGC GC^T[ACGT]AGC
2881 GCTAAGC
Bpu1102I 745 GCTGAGC GC^T[ACGT]AGC
2881 GCTAAGC
BsaWI 1377 TCCGGA [AT]^CCGG[AT]
2763 ACCGGT
BspLU11I 2541 ACATGT A^CATGT
3028 ACATGT
Bsu36I 1108 CCTAAGG CC^T[ACGT]AGG
3375 CCTTAGG
CelII 745 GCTGAGC GC^T[ACGT]AGC
2881 GCTAAGC
Eco81I 1108 CCTAAGG CC^T[ACGT]AGG
3375 CCTTAGG
HincII 1320 GTTGAC GT[CT]^[GA]AC
2706 GTCAAC
HindII 1320 GTTGAC GT[CT]^[GA]AC
2706 GTCAAC
NcoI 3491 CCATGG C^CATGG
3726 CCATGG
PflMI 2000 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2923 CCACCACCTGG
PpuMI 3371 GGGACCT [GA]G^G[AT]CC[CT]
3823 AGGTCCT
PstI 1013 CTGCAG CTGCA^G
3746 CTGCAG
ScaI 100 AGTACT AGT^ACT
3844 AGTACT
Van91I 2000 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2923 CCACCACCTGG
XbaI 3267 TCTAGA T^CTAGA
4045 TCTAGA
XcmI 2923 CCACCACCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3632 CCATGTGGGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AclI AcsI AcyI
AfaI AluI AlwI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI Psp1406I PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI