Restriction map for
pF1KB0166
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AflIII AgeI AhdI Alw44I
ApaLI AscI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BlnI BsaAI BsaBI BseAI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CpoI DrdI Eam1105I EcoO65I EcoRI
EcoRV FseI FspI HgaI HpaI
KasI MamI MluI MroI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1865 GACGTC GACGT^C
Acc65I 34 GGTACC G^GTACC
AccI 1696 GTAGAC GT^[AC][GT]AC
AclI 246 AACGTT AA^CGTT
AcyI 1865 GACGTC G[GA]^CG[CT]C
AflII 2458 CTTAAG C^TTAAG
AseI 2821 ATTAAT AT^TAAT
AsnI 2821 ATTAAT AT^TAAT
Asp718I 34 GGTACC G^GTACC
AspI 1984 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2077 GTATCC GTATCC(6/5)
BfrI 2458 CTTAAG C^TTAAG
BglI 735 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1865 GACGTC G[GA]^CG[CT]C
BsaWI 3357 ACCGGA [AT]^CCGG[AT]
BsmI 3335 GCATTC GAATGC(1/-1)
BspDI 2425 ATCGAT AT^CGAT
BspHI 2793 TCATGA T^CATGA
BspMI 3371 ACCTGC ACCTGC(4/8)
ClaI 2425 ATCGAT AT^CGAT
DraI 3324 TTTAAA TTT^AAA
DraIII 434 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 156 CGGCCG C^GGCCG
EclXI 156 CGGCCG C^GGCCG
Eco52I 156 CGGCCG C^GGCCG
EcoNI 732 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2290 CCATGG C^C[AT][AT]GG
EcoT22I 917 ATGCAT ATGCA^T
Hin1I 1865 GACGTC G[GA]^CG[CT]C
KpnI 34 GGTACC GGTAC^C
KspI 22 CCGCGG CCGC^GG
MfeI 3275 CAATTG C^AATTG
MslI 448 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3275 CAATTG C^AATTG
NaeI 158 GCCGGC GCC^GGC
NcoI 2290 CCATGG C^CATGG
NgoMIV 158 GCCGGC G^CCGGC
NheI 94 GCTAGC G^CTAGC
NsiI 917 ATGCAT ATGCA^T
PflFI 1984 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2153 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 40 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1406 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2821 ATTAAT AT^TAAT
Psp1406I 246 AACGTT AA^CGTT
RcaI 2793 TCATGA T^CATGA
SacII 22 CCGCGG CCGC^GG
ScaI 3350 AGTACT AGT^ACT
SmaI 30 CCCGGG CCC^GGG
StyI 2290 CCATGG C^C[AT][AT]GG
Tth111I 1984 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2153 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1393 TCTAGA T^CTAGA
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 23 CGCG CG^CG
179 CGCG
Aor51HI 2657 AGCGCT AGC^GCT
2996 AGCGCT
ApaI 51 GGGCCC GGGCC^C
70 GGGCCC
Asp700I 770 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAATATGTTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
67 CTCGGG
BalI 559 TGGCCA TGG^CCA
1901 TGGCCA
BanI 34 GGTACC G^G[CT][GA]CC
1363 GGTGCC
BclI 2174 TGATCA T^GATCA
2679 TGATCA
BglII 2532 AGATCT A^GATCT
3308 AGATCT
BlpI 171 GCTAAGC GC^T[ACGT]AGC
2986 GCTGAGC
Bpu1102I 171 GCTAAGC GC^T[ACGT]AGC
2986 GCTGAGC
BsaI 1513 GAGACC GGTCTC(1/5)
3016 GAGACC
BseRI 2543 CTCCTC GAGGAG(10/8)
2119 GAGGAG
BsgI 3080 CTGCAC GTGCAG(16/14)
643 GTGCAG
BsiEI 152 CGGTCG CG[GA][CT]^CG
156 CGGCCG
BsoBI 30 CCCGGG C^[CT]CG[GA]G
67 CTCGGG
BsrFI 150 GCCGGT [GA]^CCGG[CT]
158 GCCGGC
BstAPI 726 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
744 GCAAGGCATGC
BstUI 23 CGCG CG^CG
179 CGCG
CelII 171 GCTAAGC GC^T[ACGT]AGC
2986 GCTGAGC
CfoI 2658 GCGC GCG^C
2997 GCGC
Cfr10I 150 GCCGGT [GA]^CCGG[CT]
158 GCCGGC
DraII 40 GGGTCCC [GA]G^G[ACGT]CC[CT]
50 GGGGCCC
Eco47III 2657 AGCGCT AGC^GCT
2996 AGCGCT
EcoO109I 40 GGGTCCC [GA]G^G[ACGT]CC[CT]
50 GGGGCCC
FauI 54 CCCGC CCCGC(4/6)
180 GCGGG
FbaI 2174 TGATCA T^GATCA
2679 TGATCA
HaeII 2657 AGCGCT [GA]GCGC^[CT]
2996 AGCGCT
HhaI 2658 GCGC GCG^C
2997 GCGC
HinP1I 2658 GCGC G^CGC
2997 GCGC
MluNI 559 TGGCCA TGG^CCA
1901 TGGCCA
MscI 559 TGGCCA TGG^CCA
1901 TGGCCA
MvnI 23 CGCG CG^CG
179 CGCG
SacI 705 GAGCTC GAGCT^C
1627 GAGCTC
SspI 2188 AATATT AAT^ATT
3446 AATATT
XmnI 770 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI Eco81I EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII