Restriction map for
pF1KB0056
##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BbeI BbrPI BcgI BfrI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NarI NdeI NotI
NruI NsiI PacI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacI ScaI SfiI SfoI SspBI
SspI SwaI Tru9I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 345 GACGCC G[GA]^CG[CT]C
AgeI 2349 ACCGGT A^CCGGT
Asp700I 1807 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3392 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4020 TGGCCA TGG^CCA
BamHI 2355 GGATCC G^GATCC
BanI 1494 GGCACC G^G[CT][GA]CC
BclI 945 TGATCA T^GATCA
BglII 3668 AGATCT A^GATCT
BsaAI 72 TACGTA [CT]AC^GT[GA]
BsaHI 345 GACGCC G[GA]^CG[CT]C
BsaI 3252 GAGACC GGTCTC(1/5)
BsaWI 2349 ACCGGT [AT]^CCGG[AT]
BsiEI 6 CGATCG CG[GA][CT]^CG
BsmBI 4393 CGTCTC CGTCTC(1/5)
BsmI 3207 GAATGC GAATGC(1/-1)
BspHI 684 TCATGA T^CATGA
BssSI 3515 CACGAG CACGAG(-5/-1)
BstBI 1750 TTCGAA TT^CGAA
DrdI 217 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 945 TGATCA T^GATCA
FseI 2125 GGCCGGCC GGCCGG^CC
Hin1I 345 GACGCC G[GA]^CG[CT]C
KspI 738 CCGCGG CCGC^GG
MluNI 4020 TGGCCA TGG^CCA
MscI 4020 TGGCCA TGG^CCA
NheI 4255 GCTAGC G^CTAGC
NspI 1430 GCATGC [GA]CATG^[CT]
NspV 1750 TTCGAA TT^CGAA
PflFI 3392 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2349 ACCGGT A^CCGGT
PvuI 6 CGATCG CGAT^CG
PvuII 4087 CAGCTG CAG^CTG
RcaI 684 TCATGA T^CATGA
SacII 738 CCGCGG CCGC^GG
SalI 4444 GTCGAC G^TCGAC
SbfI 516 CCTGCAGG CCTGCA^GG
SexAI 3393 ACCTGGT A^CC[AT]GGT
SfuI 1750 TTCGAA TT^CGAA
SgrAI 2348 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 72 TACGTA TAC^GTA
SphI 1430 GCATGC GCATG^C
Sse8387I 516 CCTGCAGG CCTGCA^GG
Tth111I 3392 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2220 TCTAGA T^CTAGA
XmnI 1807 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4078 GTAGAC GT^[AC][GT]AC
4444 GTCGAC
AccII 739 CGCG CG^CG
4441 CGCG
AcsI 505 GAATTT [GA]^AATT[CT]
606 AAATTC
ApoI 505 GAATTT [GA]^AATT[CT]
606 AAATTC
AspHI 449 GAGCAC G[AT]GC[AT]^C
2117 GTGCTC
AvrII 2662 CCTAGG C^CTAGG
3247 CCTAGG
BbvCI 3791 CCTCAGC CCTCAGC(-5/-2)
1063 GCTGAGG
BlnI 2662 CCTAGG C^CTAGG
3247 CCTAGG
BlpI 307 GCTCAGC GC^T[ACGT]AGC
3553 GCTGAGC
Bpu1102I 307 GCTCAGC GC^T[ACGT]AGC
3553 GCTGAGC
BsiHKAI 449 GAGCAC G[AT]GC[AT]^C
2117 GTGCTC
BsrDI 153 CATTGC GCAATG(2/0)
212 GCAATG
BstUI 739 CGCG CG^CG
4441 CGCG
CelII 307 GCTCAGC GC^T[ACGT]AGC
3553 GCTGAGC
EaeI 3379 CGGCCA [CT]^GGCC[GA]
4020 TGGCCA
HaeII 1442 AGCGCC [GA]GCGC^[CT]
3617 AGCGCC
MaeII 73 ACGT A^CGT
2805 ACGT
MvnI 739 CGCG CG^CG
4441 CGCG
PaeR7I 497 CTCGAG C^TCGAG
4523 CTCGAG
SapI 1438 GAAGAGC GCTCTTC(1/4)
1639 GAAGAGC
SmaI 692 CCCGGG CCC^GGG
3296 CCCGGG
SpeI 231 ACTAGT A^CTAGT
3418 ACTAGT
StuI 3184 AGGCCT AGG^CCT
4460 AGGCCT
XhoI 497 CTCGAG C^TCGAG
4523 CTCGAG
XmaCI 692 CCCGGG C^CCGGG
3296 CCCGGG
XmaI 692 CCCGGG C^CCGGG
3296 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AvaI AvaII BanII
BbsI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII