Restriction map for
pF1KB0053
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CpoI DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT14I EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I StuI StyI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1093 GTCTAC GT^[AC][GT]AC
AhdI 3238 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 171 GGGCCC GGGCC^C
AspEI 3238 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3561 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3600 GGATCC G^GATCC
BbvCI 1558 GCTGAGG CCTCAGC(-5/-2)
BsmBI 303 GAGACG CGTCTC(1/5)
BsmFI 2796 GGGAC GGGAC(10/14)
BspDI 3626 ATCGAT AT^CGAT
BsrFI 3116 GCCGGC [GA]^CCGG[CT]
BstBI 3173 TTCGAA TT^CGAA
BstXI 1098 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3116 GCCGGC [GA]^CCGG[CT]
ClaI 3626 ATCGAT AT^CGAT
DraIII 2936 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3238 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1001 GATATC GAT^ATC
FauI 3553 CCCGC CCCGC(4/6)
HaeII 1988 GGCGCT [GA]GCGC^[CT]
HapII 3117 CCGG C^CGG
HgaI 3561 GACGC GACGC(5/10)
HindIII 2123 AAGCTT A^AGCTT
HpaII 3117 CCGG C^CGG
MaeII 2463 ACGT A^CGT
MspI 3117 CCGG C^CGG
MwoI 2817 GCTGAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 3116 GCCGGC GCC^GGC
NgoMIV 3116 GCCGGC G^CCGGC
NspV 3173 TTCGAA TT^CGAA
PflFI 3561 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3034 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1802 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 2579 GCTCTTC GCTCTTC(1/4)
ScaI 1184 AGTACT AGT^ACT
SfuI 3173 TTCGAA TT^CGAA
SpeI 700 ACTAGT A^CTAGT
Tth111I 3561 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3034 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1725 CCAAGTGCGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaII 1803 GGTCC G^G[AT]CC
3666 GGACC
BciVI 1135 GGATAC GTATCC(6/5)
2035 GTATCC
BclI 1449 TGATCA T^GATCA
2214 TGATCA
BglII 433 AGATCT A^GATCT
1101 AGATCT
BsaBI 3595 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3601 GATCCTGATC
BsgI 710 CTGCAC GTGCAG(16/14)
3930 GTGCAG
BspMI 1878 ACCTGC ACCTGC(4/8)
3268 GCAGGT
BsrDI 1220 CATTGC GCAATG(2/0)
1245 GCAATG
CfoI 1989 GCGC GCG^C
3902 GCGC
FbaI 1449 TGATCA T^GATCA
2214 TGATCA
HhaI 1989 GCGC GCG^C
3902 GCGC
HinP1I 1989 GCGC G^CGC
3902 GCGC
MamI 3595 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3601 GATCCTGATC
MfeI 943 CAATTG C^AATTG
3203 CAATTG
MslI 39 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3522 CACTGGAATG
MunI 943 CAATTG C^AATTG
3203 CAATTG
SacI 3229 GAGCTC GAGCT^C
3288 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI