Restriction map for
pF1KA1902
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I FseI
FspI Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1395 GTCTAC GT^[AC][GT]AC
AccII 435 CGCG CG^CG
AflIII 3126 ACATGT A^C[GA][CT]GT
AocI 1565 CCTCAGG CC^T[ACGT]AGG
Aor51HI 893 AGCGCT AGC^GCT
AseI 970 ATTAAT AT^TAAT
AsnI 970 ATTAAT AT^TAAT
BbvCI 2970 GCTGAGG CCTCAGC(-5/-2)
BsaAI 683 TACGTG [CT]AC^GT[GA]
BsaWI 2927 TCCGGT [AT]^CCGG[AT]
BspLU11I 3126 ACATGT A^CATGT
BsrDI 605 CATTGC GCAATG(2/0)
BstUI 435 CGCG CG^CG
BstXI 1253 CCAAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1565 CCTCAGG CC^T[ACGT]AGG
DsaI 1508 CCATGG C^C[GA][CT]GG
EarI 2956 GAAGAG CTCTTC(1/4)
Eco47III 893 AGCGCT AGC^GCT
Eco81I 1565 CCTCAGG CC^T[ACGT]AGG
EcoRI 1955 GAATTC G^AATTC
EcoRV 1468 GATATC GAT^ATC
EcoT22I 2758 ATGCAT ATGCA^T
HapII 2928 CCGG C^CGG
HgaI 3218 GCGTC GACGC(5/10)
HincII 2568 GTCAAC GT[CT]^[GA]AC
HindII 2568 GTCAAC GT[CT]^[GA]AC
HindIII 1087 AAGCTT A^AGCTT
HpaII 2928 CCGG C^CGG
Ksp632I 2956 GAAGAG CTCTTC(1/4)
MspI 2928 CCGG C^CGG
MvnI 435 CGCG CG^CG
NcoI 1508 CCATGG C^CATGG
NsiI 2758 ATGCAT ATGCA^T
PleI 2397 GACTC GAGTC(4/5)
PshBI 970 ATTAAT AT^TAAT
ScaI 466 AGTACT AGT^ACT
StuI 1041 AGGCCT AGG^CCT
SwaI 1967 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 18 enzymes) #
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BamHI 261 GGATCC G^GATCC
3039 GGATCC
BanI 3148 GGTGCC G^G[CT][GA]CC
3262 GGTGCC
BclI 195 TGATCA T^GATCA
3237 TGATCA
BglII 2547 AGATCT A^GATCT
3171 AGATCT
BsaBI 2293 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2346 GATGACCATC
BsrBI 570 CCGCTC CCGCTC(-3/-3)
2260 GAGCGG
Cac8I 833 GCGGGC GC[ACGT]^[ACGT]GC
2949 GCAAGC
DraIII 49 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2629 CACCAAGTG
FbaI 195 TGATCA T^GATCA
3237 TGATCA
HaeII 893 AGCGCT [GA]GCGC^[CT]
3227 GGCGCT
MaeII 53 ACGT A^CGT
684 ACGT
MamI 2293 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2346 GATGACCATC
PvuII 266 CAGCTG CAG^CTG
1838 CAGCTG
SspI 973 AATATT AAT^ATT
1992 AATATT
TaqI 515 TCGA T^CGA
2327 TCGA
Tsp45I 1082 GTGAC ^GT[GC]AC
2074 GTGAC
TthHB8I 515 TCGA T^CGA
2327 TCGA
XcmI 1034 CCAAATTAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1882 CCAGTGTTTAACTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII DraI
DraII EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PpuMI
PstI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI