Restriction map for
pF1KA1901
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AviII BalI BamHI BanI BbeI
BciVI BclI BfrI BglI BlpI
Bpu1102I BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 2423 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 727 CCTAGG C^CTAGG
BanII 560 GGGCTC G[GA]GC[CT]^C
BbrPI 1841 CACGTG CAC^GTG
BbvCI 802 CCTCAGC CCTCAGC(-5/-2)
BcgI 1805 GCAAAAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 727 CCTAGG C^CTAGG
BsaAI 1841 CACGTG [CT]AC^GT[GA]
BsaBI 3451 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2946 ACCGGA [AT]^CCGG[AT]
BseRI 800 CTCCTC GAGGAG(10/8)
BsmI 3532 GAATGC GAATGC(1/-1)
BspMI 1474 GCAGGT ACCTGC(4/8)
BstAPI 1840 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 840 TTCGAA TT^CGAA
DraI 715 TTTAAA TTT^AAA
EcoT14I 727 CCTAGG C^C[AT][AT]GG
HaeII 1712 AGCGCC [GA]GCGC^[CT]
HpaI 2316 GTTAAC GTT^AAC
MaeII 1842 ACGT A^CGT
MamI 3451 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1660 CATATG CA^TATG
NheI 1575 GCTAGC G^CTAGC
NspV 840 TTCGAA TT^CGAA
PflMI 2961 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1841 CACGTG CAC^GTG
PmlI 1841 CACGTG CAC^GTG
SfuI 840 TTCGAA TT^CGAA
SmaI 2075 CCCGGG CCC^GGG
StuI 3329 AGGCCT AGG^CCT
StyI 727 CCTAGG C^C[AT][AT]GG
Van91I 2961 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2395 CCAAGAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2075 CCCGGG C^CCGGG
XmaI 2075 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 351 ACATGT A^C[GA][CT]GT
3148 ACATGT
AhdI 737 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2613 GACAAAAAGTC
Asp700I 787 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2131 GAAATACTTC
AspEI 737 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2613 GACAAAAAGTC
BbsI 3283 GAAGAC GAAGAC(2/6)
1418 GTCTTC
BcnI 2075 CCCGG CC^[GC]GG
2076 CCGGG
BglII 2011 AGATCT A^GATCT
3162 AGATCT
BmyI 560 GGGCTC G[AGT]GC[ACT]^C
3473 GTGCCC
BpuAI 3283 GAAGAC GAAGAC(2/6)
1418 GTCTTC
Bsp1286I 560 GGGCTC G[AGT]GC[ACT]^C
3473 GTGCCC
BspHI 2220 TCATGA T^CATGA
3636 TCATGA
BspLU11I 351 ACATGT A^CATGT
3148 ACATGT
CfoI 1713 GCGC GCG^C
1803 GCGC
Eam1105I 737 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2613 GACAAAAAGTC
EcoRI 1433 GAATTC G^AATTC
3084 GAATTC
HapII 2076 CCGG C^CGG
2947 CCGG
HhaI 1713 GCGC GCG^C
1803 GCGC
HinP1I 1713 GCGC G^CGC
1803 GCGC
HpaII 2076 CCGG C^CGG
2947 CCGG
MspI 2076 CCGG C^CGG
2947 CCGG
NciI 2075 CCCGG CC^[GC]GG
2076 CCGGG
PleI 2849 GAGTC GAGTC(4/5)
3388 GAGTC
RcaI 2220 TCATGA T^CATGA
3636 TCATGA
SapI 1915 GAAGAGC GCTCTTC(1/4)
2399 GAAGAGC
ScaI 463 AGTACT AGT^ACT
2857 AGTACT
SexAI 629 ACCTGGT A^CC[AT]GGT
3360 ACCTGGT
XmnI 787 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2131 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PaeR7I PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoI XhoII