Restriction map for
pF1KA1825
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# NO CUT ENZYMES ( 58 enzymes) #
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AatII AclI AflII AgeI AhdI
AocI AscI AseI AsnI AspEI
AvrII BciVI BfrI BlnI BlpI
Bpu1102I BsaBI BsiWI BspDI Bst1107I
BstZ17I Bsu36I CelII ClaI DraI
Eam1105I Eco81I EcoRV EcoT22I FseI
HindIII HpaI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SapI SfiI SgrAI SnaBI
SpeI SspI SwaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1396 GGTACC G^GTACC
Aor51HI 1865 AGCGCT AGC^GCT
Asp700I 1818 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1396 GGTACC G^GTACC
AviII 1280 TGCGCA TGC^GCA
BamHI 1559 GGATCC G^GATCC
BbvCI 1098 CCTCAGC CCTCAGC(-5/-2)
BcgI 1024 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1768 CTAG C^TAG
BglII 2030 AGATCT A^GATCT
BsmI 2211 GAATGC GAATGC(1/-1)
BspHI 2231 TCATGA T^CATGA
BstAPI 846 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 514 TTCGAA TT^CGAA
CpoI 1246 CGGACCG CG^G[AT]CCG
DraIII 2221 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1671 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1865 AGCGCT AGC^GCT
EcoRI 517 GAATTC G^AATTC
FspI 1280 TGCGCA TGC^GCA
KpnI 1396 GGTACC GGTAC^C
MaeI 1768 CTAG C^TAG
MseI 1838 TTAA T^TAA
NcoI 3320 CCATGG C^CATGG
NspV 514 TTCGAA TT^CGAA
PaeR7I 2821 CTCGAG C^TCGAG
PflMI 2220 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2231 TCATGA T^CATGA
RsrII 1246 CGGACCG CG^G[AT]CCG
SacI 460 GAGCTC GAGCT^C
SbfI 2454 CCTGCAGG CCTGCA^GG
ScaI 782 AGTACT AGT^ACT
SexAI 995 ACCTGGT A^CC[AT]GGT
SfuI 514 TTCGAA TT^CGAA
Sse8387I 2454 CCTGCAGG CCTGCA^GG
StuI 830 AGGCCT AGG^CCT
Tru9I 1838 TTAA T^TAA
Van91I 2220 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1767 TCTAGA T^CTAGA
XhoI 2821 CTCGAG C^TCGAG
XmnI 1818 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 872 TCCGGA T^CCGGA
1556 TCCGGA
AcyI 188 GGCGCC G[GA]^CG[CT]C
2608 GGCGCC
AflIII 860 ACATGT A^C[GA][CT]GT
1535 ACATGT
AspI 961 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3562 GACCGCGTC
BbeI 188 GGCGCC GGCGC^C
2608 GGCGCC
BbrPI 2266 CACGTG CAC^GTG
2914 CACGTG
BclI 2234 TGATCA T^GATCA
2918 TGATCA
BsaHI 188 GGCGCC G[GA]^CG[CT]C
2608 GGCGCC
BseAI 872 TCCGGA T^CCGGA
1556 TCCGGA
BsmBI 966 CGTCTC CGTCTC(1/5)
1655 GAGACG
Bsp1407I 3155 TGTACA T^GTACA
3269 TGTACA
BspEI 872 TCCGGA T^CCGGA
1556 TCCGGA
BspLU11I 860 ACATGT A^CATGT
1535 ACATGT
BsrGI 3155 TGTACA T^GTACA
3269 TGTACA
EcoNI 2783 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2799 CCTGAGCCAGG
FbaI 2234 TGATCA T^GATCA
2918 TGATCA
Hin1I 188 GGCGCC G[GA]^CG[CT]C
2608 GGCGCC
KasI 188 GGCGCC G^GCGCC
2608 GGCGCC
MroI 872 TCCGGA T^CCGGA
1556 TCCGGA
NarI 188 GGCGCC GG^CGCC
2608 GGCGCC
PflFI 961 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3562 GACCGCGTC
PmaCI 2266 CACGTG CAC^GTG
2914 CACGTG
PmlI 2266 CACGTG CAC^GTG
2914 CACGTG
SfoI 188 GGCGCC GGC^GCC
2608 GGCGCC
SphI 2260 GCATGC GCATG^C
2619 GCATGC
SspBI 3155 TGTACA T^GTACA
3269 TGTACA
TfiI 1132 GATTC G^A[AT]TC
1253 GATTC
Tth111I 961 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3562 GACCGCGTC
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# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI