Restriction map for
pF1KA1714
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI ApaI AscI Asp700I Asp718I
AspI AviII BalI BbsI BbvCI
BciVI BglI BglII BpuAI BsaWI
BseAI BsiEI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstZ17I Cfr10I ClaI EagI
EclXI Eco52I EcoRV FseI FspI
HpaI KpnI MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NgoMIV NheI NotI NruI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AflII 1267 CTTAAG C^TTAAG
AflIII 2447 ACGTGT A^C[GA][CT]GT
AhdI 1168 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 424 GTGCAC G^TGCAC
AocI 1539 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2021 AGCGCT AGC^GCT
ApaLI 424 GTGCAC G^TGCAC
AseI 705 ATTAAT AT^TAAT
AsnI 705 ATTAAT AT^TAAT
AspEI 1168 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 165 CCCGGG C^[CT]CG[GA]G
BamHI 1572 GGATCC G^GATCC
BbeI 2063 GGCGCC GGCGC^C
BbrPI 2632 CACGTG CAC^GTG
BcgI 3155 CGAGCTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1267 CTTAAG C^TTAAG
BlpI 748 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTAAGC GC^T[ACGT]AGC
BsaAI 2632 CACGTG [CT]AC^GT[GA]
BsiWI 1973 CGTACG C^GTACG
BsoBI 165 CCCGGG C^[CT]CG[GA]G
BspHI 1064 TCATGA T^CATGA
BssSI 2075 CACGAG CACGAG(-5/-1)
BstAPI 3004 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1193 TTCGAA TT^CGAA
Bsu36I 1539 CCTAAGG CC^T[ACGT]AGG
CelII 748 GCTAAGC GC^T[ACGT]AGC
CpoI 1999 CGGTCCG CG^G[AT]CCG
DraII 1613 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1168 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2021 AGCGCT AGC^GCT
Eco81I 1539 CCTAAGG CC^T[ACGT]AGG
EcoNI 113 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1613 GGGACCT [GA]G^G[ACGT]CC[CT]
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
KasI 2063 GGCGCC G^GCGCC
NarI 2063 GGCGCC GG^CGCC
NdeI 1390 CATATG CA^TATG
NspI 2532 GCATGC [GA]CATG^[CT]
NspV 1193 TTCGAA TT^CGAA
PacI 706 TTAATTAA TTAAT^TAA
PmaCI 2632 CACGTG CAC^GTG
PmlI 2632 CACGTG CAC^GTG
PpuMI 1613 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 705 ATTAAT AT^TAAT
RcaI 1064 TCATGA T^CATGA
RsrII 1999 CGGTCCG CG^G[AT]CCG
SapI 2745 GCTCTTC GCTCTTC(1/4)
SfoI 2063 GGCGCC GGC^GCC
SfuI 1193 TTCGAA TT^CGAA
SmaI 165 CCCGGG CCC^GGG
SpeI 2239 ACTAGT A^CTAGT
SphI 2532 GCATGC GCATG^C
XmaCI 165 CCCGGG C^CCGGG
XmaI 165 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvrII 118 CCTAGG C^CTAGG
490 CCTAGG
BclI 2676 TGATCA T^GATCA
3122 TGATCA
BlnI 118 CCTAGG C^CTAGG
490 CCTAGG
BsaBI 507 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3247 GATAGACATC
BsaI 1741 GAGACC GGTCTC(1/5)
2798 GGTCTC
BsmBI 2663 CGTCTC CGTCTC(1/5)
614 GAGACG
BstXI 902 CCAGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2089 CCACTGAGCTGG
EaeI 2223 TGGCCG [CT]^GGCC[GA]
2762 CGGCCA
EcoT22I 2790 ATGCAT ATGCA^T
3266 ATGCAT
FbaI 2676 TGATCA T^GATCA
3122 TGATCA
HindIII 718 AAGCTT A^AGCTT
981 AAGCTT
KspI 2003 CCGCGG CCGC^GG
2355 CCGCGG
MamI 507 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3247 GATAGACATC
NsiI 2790 ATGCAT ATGCA^T
3266 ATGCAT
PstI 2803 CTGCAG CTGCA^G
2883 CTGCAG
PvuII 1418 CAGCTG CAG^CTG
1993 CAGCTG
SacII 2003 CCGCGG CCGC^GG
2355 CCGCGG
SmlI 25 CTCAAG C^T[CT][GA]AG
1267 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DrdI DsaI EarI EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII