Restriction map for
pF1KA1687
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 367 CGCG CG^CG
AciI 573 CCGC CCGC(-3/-1)
AseI 1009 ATTAAT AT^TAAT
AsnI 1009 ATTAAT AT^TAAT
Asp700I 339 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1799 GTGCTC G[AT]GC[AT]^C
BcnI 138 CCCGG CC^[GC]GG
BsaI 1027 GAGACC GGTCTC(1/5)
BsiHKAI 1799 GTGCTC G[AT]GC[AT]^C
BsmBI 1451 GAGACG CGTCTC(1/5)
BsmI 450 GAATGC GAATGC(1/-1)
Bsp1407I 886 TGTACA T^GTACA
BspHI 1590 TCATGA T^CATGA
BspMI 149 GCAGGT ACCTGC(4/8)
BsrFI 237 ACCGGC [GA]^CCGG[CT]
BsrGI 886 TGTACA T^GTACA
BstUI 367 CGCG CG^CG
Cac8I 765 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 237 ACCGGC [GA]^CCGG[CT]
DraI 1458 TTTAAA TTT^AAA
EaeI 1389 TGGCCG [CT]^GGCC[GA]
EcoNI 73 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 955 GAATTC G^AATTC
HindIII 353 AAGCTT A^AGCTT
HpaI 2107 GTTAAC GTT^AAC
MaeIII 1137 GTTAC ^GT[ACGT]AC
MfeI 736 CAATTG C^AATTG
MslI 1008 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 787 CAGCTG C[AC]G^C[GT]G
MunI 736 CAATTG C^AATTG
MvnI 367 CGCG CG^CG
NciI 138 CCCGG CC^[GC]GG
NdeI 2066 CATATG CA^TATG
NspI 2128 GCATGT [GA]CATG^[CT]
PaeR7I 1976 CTCGAG C^TCGAG
PshAI 792 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1009 ATTAAT AT^TAAT
PvuII 787 CAGCTG CAG^CTG
RcaI 1590 TCATGA T^CATGA
SnaBI 1654 TACGTA TAC^GTA
SspBI 886 TGTACA T^GTACA
XhoI 1976 CTCGAG C^TCGAG
XmnI 339 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 887 GTAC GT^AC
1325 GTAC
AvaI 167 CTCGGG C^[CT]CG[GA]G
1976 CTCGAG
BcgI 498 GCAAACTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1248 GCAAAGCCCTCG
BfaI 1268 CTAG C^TAG
1676 CTAG
BglII 460 AGATCT A^GATCT
1146 AGATCT
BpmI 1899 CTCCAG CTGGAG(16/14)
54 CTGGAG
BsaAI 1654 TACGTA [CT]AC^GT[GA]
2017 TACGTG
BsoBI 167 CTCGGG C^[CT]CG[GA]G
1976 CTCGAG
BsrDI 1894 CATTGC GCAATG(2/0)
625 GCAATG
BstAPI 1062 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1288 GCACTTTATGC
BstNI 232 CCAGG CC^[AT]GG
1901 CCAGG
CfoI 368 GCGC GCG^C
602 GCGC
DraII 133 GGGACCC [GA]G^G[ACGT]CC[CT]
1628 GGGACCC
EcoO109I 133 GGGACCC [GA]G^G[ACGT]CC[CT]
1628 GGGACCC
EcoRII 232 CCAGG ^CC[AT]GG
1901 CCAGG
EcoT14I 704 CCTTGG C^C[AT][AT]GG
879 CCAAGG
HaeIII 240 GGCC GG^CC
1390 GGCC
HapII 139 CCGG C^CGG
238 CCGG
HhaI 368 GCGC GCG^C
602 GCGC
HinP1I 368 GCGC G^CGC
602 GCGC
HpaII 139 CCGG C^CGG
238 CCGG
MaeI 1268 CTAG C^TAG
1676 CTAG
MspI 139 CCGG C^CGG
238 CCGG
MvaI 232 CCAGG CC^[AT]GG
1901 CCAGG
PflMI 585 CCATAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1358 CCAACAGTTGG
PpuMI 133 GGGACCC [GA]G^G[AT]CC[CT]
1628 GGGACCC
PstI 770 CTGCAG CTGCA^G
784 CTGCAG
RsaI 887 GTAC GT^AC
1325 GTAC
SmlI 1180 CTTGAG C^T[CT][GA]AG
1976 CTCGAG
StyI 704 CCTTGG C^C[AT][AT]GG
879 CCAAGG
TaqI 861 TCGA T^CGA
1977 TCGA
TthHB8I 861 TCGA T^CGA
1977 TCGA
Van91I 585 CCATAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1358 CCAACAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AluI AlwI AlwNI AocI
ApoI AvaII BanI BbsI BbvI
BmyI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstYI Bsu36I Cfr13I
DdeI DpnII EarI Eco81I Fnu4HI
FokI HincII HindII HinfI HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV PleI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XhoII