Restriction map for
pF1KA1430
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsgI BsiWI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshBI
PstI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AlwNI 572 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 733 CCTGAGG CC^T[ACGT]AGG
AspHI 1271 GTGCTC G[AT]GC[AT]^C
AvaI 1117 CCCGGG C^[CT]CG[GA]G
BbrPI 337 CACGTG CAC^GTG
BbvI 919 GCAGC GCAGC(8/12)
BfaI 1235 CTAG C^TAG
BmyI 1271 GTGCTC G[AGT]GC[ACT]^C
BsaAI 337 CACGTG [CT]AC^GT[GA]
BsaI 1501 GGTCTC GGTCTC(1/5)
BsaJI 1117 CCCGGG C^C[ACGT][ACGT]GG
BsiEI 1161 CGATCG CG[GA][CT]^CG
BsiHKAI 1271 GTGCTC G[AT]GC[AT]^C
BsmBI 599 CGTCTC CGTCTC(1/5)
BsmFI 767 GTCCC GGGAC(10/14)
BsmI 167 GAATGC GAATGC(1/-1)
BsoBI 1117 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1271 GTGCTC G[AGT]GC[ACT]^C
BspDI 1159 ATCGAT AT^CGAT
BspLU11I 627 ACATGT A^CATGT
BsrDI 278 CATTGC GCAATG(2/0)
BstBI 156 TTCGAA TT^CGAA
Bsu36I 733 CCTGAGG CC^T[ACGT]AGG
Cac8I 282 GCCAGC GC[ACGT]^[ACGT]GC
ClaI 1159 ATCGAT AT^CGAT
Eco81I 733 CCTGAGG CC^T[ACGT]AGG
EcoRI 356 GAATTC G^AATTC
Fnu4HI 919 GCAGC GC^[ACGT]GC
HapII 1118 CCGG C^CGG
HincII 1531 GTTGAC GT[CT]^[GA]AC
HindII 1531 GTTGAC GT[CT]^[GA]AC
HindIII 1052 AAGCTT A^AGCTT
HpaII 1118 CCGG C^CGG
ItaI 919 GCAGC GC^[ACGT]GC
MaeI 1235 CTAG C^TAG
MspA1I 1526 CAGCGG C[AC]G^C[GT]G
MspI 1118 CCGG C^CGG
NspI 627 ACATGT [GA]CATG^[CT]
NspV 156 TTCGAA TT^CGAA
PmaCI 337 CACGTG CAC^GTG
PmlI 337 CACGTG CAC^GTG
PshAI 760 GACGTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1161 CGATCG CGAT^CG
ScaI 706 AGTACT AGT^ACT
SfaNI 568 GATGC GCATC(5/9)
SfuI 156 TTCGAA TT^CGAA
SmaI 1117 CCCGGG CCC^GGG
SmlI 1336 CTTGAG C^T[CT][GA]AG
SspI 908 AATATT AAT^ATT
TseI 919 GCAGC G^C[AT]GC
XmaCI 1117 CCCGGG C^CCGGG
XmaI 1117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AclI 469 AACGTT AA^CGTT
1367 AACGTT
AvaII 36 GGACC G^G[AT]CC
1078 GGACC
BbsI 1559 GAAGAC GAAGAC(2/6)
969 GTCTTC
BcnI 1117 CCCGG CC^[GC]GG
1118 CCGGG
BpuAI 1559 GAAGAC GAAGAC(2/6)
969 GTCTTC
BsaBI 580 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATCTGAATC
BstF5I 1249 CATCC GGATG(2/0)
1484 CATCC
BstNI 1213 CCAGG CC^[AT]GG
1478 CCAGG
BstYI 1032 GGATCT [GA]^GATC[CT]
1441 AGATCC
EcoRII 1213 CCAGG ^CC[AT]GG
1478 CCAGG
FokI 1249 CATCC GGATG(9/13)
1484 CATCC
MamI 580 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATCTGAATC
MflI 1032 GGATCT [GA]^GATC[CT]
1441 AGATCC
MslI 162 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
713 CACCAAAATG
MvaI 1213 CCAGG CC^[AT]GG
1478 CCAGG
NciI 1117 CCCGG CC^[GC]GG
1118 CCGGG
PflMI 715 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1351 CCAACAGTTGG
PleI 982 GACTC GAGTC(4/5)
739 GAGTC
Psp1406I 469 AACGTT AA^CGTT
1367 AACGTT
TfiI 595 GATTC G^A[AT]TC
1038 GAATC
Van91I 715 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1351 CCAACAGTTGG
XhoII 1032 GGATCT [GA]^GATC[CT]
1441 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BalI BclI BpmI
BseRI BsiYI BslI BsmAI BsrI
BssKI Cfr13I DdeI DpnII EaeI
EarI FbaI HaeIII HinfI HphI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI TaqI Tru9I Tsp45I Tsp509I
TspRI TthHB8I