Restriction map for
pF1KA1426
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# NO CUT ENZYMES ( 73 enzymes) #
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AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbeI BciVI BfrI
BglI BglII BlnI BsiWI BspDI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoRV EcoT22I FseI
HpaI KasI MfeI MluI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmeI PshAI
PshBI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I Tth111I
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 2599 GACGTC GACGT^C
Acc65I 2843 GGTACC G^GTACC
AccI 1459 GTCTAC GT^[AC][GT]AC
AccIII 844 TCCGGA T^CCGGA
AclI 2098 AACGTT AA^CGTT
AcsI 466 GAATTC [GA]^AATT[CT]
ApoI 466 GAATTC [GA]^AATT[CT]
Asp718I 2843 GGTACC G^GTACC
AviII 146 TGCGCA TGC^GCA
BbrPI 706 CACGTG CAC^GTG
BcgI 1889 CGACAACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 75 TGATCA T^GATCA
BsaAI 706 CACGTG [CT]AC^GT[GA]
BsaBI 2697 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 450 GGTCTC GGTCTC(1/5)
BseAI 844 TCCGGA T^CCGGA
BsmI 180 GAATGC GAATGC(1/-1)
Bsp1407I 598 TGTACA T^GTACA
BspEI 844 TCCGGA T^CCGGA
BspHI 1466 TCATGA T^CATGA
BsrDI 1856 GCAATG GCAATG(2/0)
BsrGI 598 TGTACA T^GTACA
BssSI 2229 CACGAG CACGAG(-5/-1)
BstEII 1704 GGTGACC G^GT[ACGT]ACC
BstPI 1704 GGTGACC G^GT[ACGT]ACC
BstYI 725 AGATCC [GA]^GATC[CT]
EcoO65I 1704 GGTGACC G^GT[ACGT]ACC
EcoRI 466 GAATTC G^AATTC
FbaI 75 TGATCA T^GATCA
FspI 146 TGCGCA TGC^GCA
HincII 1609 GTCAAC GT[CT]^[GA]AC
HindII 1609 GTCAAC GT[CT]^[GA]AC
HindIII 2889 AAGCTT A^AGCTT
KpnI 2843 GGTACC GGTAC^C
MamI 2697 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 725 AGATCC [GA]^GATC[CT]
MroI 844 TCCGGA T^CCGGA
PflMI 723 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 706 CACGTG CAC^GTG
PmlI 706 CACGTG CAC^GTG
Psp1406I 2098 AACGTT AA^CGTT
PvuI 2198 CGATCG CGAT^CG
RcaI 1466 TCATGA T^CATGA
SspBI 598 TGTACA T^GTACA
TfiI 3040 GATTC G^A[AT]TC
Tsp509I 467 AATT ^AATT
Van91I 723 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1059 TCTAGA T^CTAGA
XhoII 725 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 634 GTGCAC G^TGCAC
658 GTGCAC
ApaLI 634 GTGCAC G^TGCAC
658 GTGCAC
Asp700I 199 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1920 GAAGCTCTTC
BalI 1023 TGGCCA TGG^CCA
2240 TGGCCA
BbvCI 1575 CCTCAGC CCTCAGC(-5/-2)
790 GCTGAGG
BfaI 1060 CTAG C^TAG
2072 CTAG
BsaWI 844 TCCGGA [AT]^CCGG[AT]
2710 TCCGGT
BsmBI 1340 CGTCTC CGTCTC(1/5)
2601 CGTCTC
BsrBI 523 GAGCGG CCGCTC(-3/-3)
783 GAGCGG
DrdI 1096 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2431 GACCCATTTGTC
EcoNI 967 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1652 CCTCAGTAAGG
MaeI 1060 CTAG C^TAG
2072 CTAG
MluNI 1023 TGGCCA TGG^CCA
2240 TGGCCA
MscI 1023 TGGCCA TGG^CCA
2240 TGGCCA
MslI 1977 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2392 CACCAGGATG
NaeI 1388 GCCGGC GCC^GGC
2272 GCCGGC
NgoMIV 1388 GCCGGC G^CCGGC
2272 GCCGGC
SapI 2907 GAAGAGC GCTCTTC(1/4)
1923 GCTCTTC
XcmI 1616 CCAAGCTGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCACGAGTCACCTGG
XmnI 199 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1920 GAAGCTCTTC
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoI XmaCI
XmaI