Restriction map for
pF1KA1336
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AccII AccIII AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AspI AvaII AvrII BalI
BanII BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FauI FseI HpaI
KspI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NciI NdeI NgoMIV NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI Sse8387I StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 635 GGTACC G^GTACC
AclI 1224 AACGTT AA^CGTT
AcyI 2096 GGCGCC G[GA]^CG[CT]C
AflIII 2895 ACGTGT A^C[GA][CT]GT
Aor51HI 2195 AGCGCT AGC^GCT
AseI 1393 ATTAAT AT^TAAT
AsnI 1393 ATTAAT AT^TAAT
Asp718I 635 GGTACC G^GTACC
AspHI 2024 GAGCAC G[AT]GC[AT]^C
AviII 3220 TGCGCA TGC^GCA
BbeI 2096 GGCGCC GGCGC^C
BclI 326 TGATCA T^GATCA
BlpI 2492 GCTTAGC GC^T[ACGT]AGC
BmyI 2024 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 2492 GCTTAGC GC^T[ACGT]AGC
BsaHI 2096 GGCGCC G[GA]^CG[CT]C
BsiHKAI 2024 GAGCAC G[AT]GC[AT]^C
BsmBI 130 GAGACG CGTCTC(1/5)
Bsp1286I 2024 GAGCAC G[AGT]GC[ACT]^C
Bst1107I 467 GTATAC GTA^TAC
BstZ17I 467 GTATAC GTA^TAC
CelII 2492 GCTTAGC GC^T[ACGT]AGC
DraII 158 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 639 CCATGG C^C[GA][CT]GG
Eco47III 2195 AGCGCT AGC^GCT
EcoNI 3486 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 158 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 626 GAATTC G^AATTC
FbaI 326 TGATCA T^GATCA
FspI 3220 TGCGCA TGC^GCA
HapII 2319 CCGG C^CGG
HgaI 132 GACGC GACGC(5/10)
Hin1I 2096 GGCGCC G[GA]^CG[CT]C
HindIII 3257 AAGCTT A^AGCTT
HpaII 2319 CCGG C^CGG
KasI 2096 GGCGCC G^GCGCC
KpnI 635 GGTACC GGTAC^C
MspI 2319 CCGG C^CGG
NarI 2096 GGCGCC GG^CGCC
NcoI 639 CCATGG C^CATGG
NheI 3210 GCTAGC G^CTAGC
PshBI 1393 ATTAAT AT^TAAT
Psp1406I 1224 AACGTT AA^CGTT
ScaI 2346 AGTACT AGT^ACT
SfoI 2096 GGCGCC GGC^GCC
SnaBI 763 TACGTA TAC^GTA
SpeI 1233 ACTAGT A^CTAGT
XcmI 2999 CCACTTCTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AciI 92 GCGG CCGC(-3/-1)
496 GCGG
AocI 1189 CCTCAGG CC^T[ACGT]AGG
1873 CCTGAGG
AvaI 1721 CTCGAG C^[CT]CG[GA]G
2284 CTCGAG
BciVI 1083 GGATAC GTATCC(6/5)
58 GTATCC
BglII 386 AGATCT A^GATCT
3197 AGATCT
BpmI 2350 CTCCAG CTGGAG(16/14)
605 CTGGAG
BsaBI 433 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1177 GATGAAAATC
BseRI 319 GAGGAG GAGGAG(10/8)
1195 GAGGAG
BsmFI 1740 GGGAC GGGAC(10/14)
3244 GGGAC
BsoBI 1721 CTCGAG C^[CT]CG[GA]G
2284 CTCGAG
Bsp1407I 2457 TGTACA T^GTACA
2469 TGTACA
BspMI 2671 ACCTGC ACCTGC(4/8)
2255 GCAGGT
BsrGI 2457 TGTACA T^GTACA
2469 TGTACA
BstAPI 2623 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3464 GCAGTGTATGC
Bsu36I 1189 CCTCAGG CC^T[ACGT]AGG
1873 CCTGAGG
Cfr13I 159 GGGCC G^G[ACGT]CC
1802 GGGCC
DrdI 499 GACAGTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1049 GACCTGATCGTC
Eco81I 1189 CCTCAGG CC^T[ACGT]AGG
1873 CCTGAGG
HaeII 2096 GGCGCC [GA]GCGC^[CT]
2195 AGCGCT
HincII 255 GTTGAC GT[CT]^[GA]AC
2648 GTCAAC
HindII 255 GTTGAC GT[CT]^[GA]AC
2648 GTCAAC
MamI 433 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1177 GATGAAAATC
PaeR7I 1721 CTCGAG C^TCGAG
2284 CTCGAG
PleI 195 GACTC GAGTC(4/5)
2218 GAGTC
PstI 49 CTGCAG CTGCA^G
2141 CTGCAG
SapI 2116 GCTCTTC GCTCTTC(1/4)
3154 GCTCTTC
Sau96I 159 GGGCC G^G[ACGT]CC
1802 GGGCC
SmlI 1721 CTCGAG C^T[CT][GA]AG
2284 CTCGAG
SphI 88 GCATGC GCATG^C
3223 GCATGC
SspBI 2457 TGTACA T^GTACA
2469 TGTACA
Tsp45I 601 GTCAC ^GT[GC]AC
2626 GTGAC
XhoI 1721 CTCGAG C^TCGAG
2284 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BamHI BanI
BbsI BbvI BfaI BpuAI BsaAI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
DdeI DpnII EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI