Restriction map for
pF1KA1319
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BglII BlnI BsaAI
BsaBI BsiEI BsiWI Bsp1407I BspDI
BspHI BspMI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SfiI
SfoI SnaBI SpeI SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 839 CGCG CG^CG
AccIII 1904 TCCGGA T^CCGGA
AcyI 119 GACGCC G[GA]^CG[CT]C
AflII 1921 CTTAAG C^TTAAG
AhdI 2244 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 848 GTGCAC G^TGCAC
ApaLI 848 GTGCAC G^TGCAC
AspEI 2244 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3101 TGGCCA TGG^CCA
BamHI 441 GGATCC G^GATCC
BanI 9 GGCACC G^G[CT][GA]CC
BfrI 1921 CTTAAG C^TTAAG
BsaHI 119 GACGCC G[GA]^CG[CT]C
BsaWI 1904 TCCGGA [AT]^CCGG[AT]
BseAI 1904 TCCGGA T^CCGGA
BsmBI 2164 GAGACG CGTCTC(1/5)
BsmI 2343 GAATGC GAATGC(1/-1)
BspEI 1904 TCCGGA T^CCGGA
BsrDI 3564 CATTGC GCAATG(2/0)
BsrFI 2207 GCCGGC [GA]^CCGG[CT]
BstBI 741 TTCGAA TT^CGAA
BstUI 839 CGCG CG^CG
Cfr10I 2207 GCCGGC [GA]^CCGG[CT]
DrdI 43 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2244 GACTCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 119 GACGCC G[GA]^CG[CT]C
HindIII 3476 AAGCTT A^AGCTT
KspI 838 CCGCGG CCGC^GG
MaeII 105 ACGT A^CGT
MluNI 3101 TGGCCA TGG^CCA
MroI 1904 TCCGGA T^CCGGA
MscI 3101 TGGCCA TGG^CCA
MslI 3283 CATGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 839 CGCG CG^CG
NaeI 2207 GCCGGC GCC^GGC
NcoI 45 CCATGG C^CATGG
NdeI 937 CATATG CA^TATG
NgoMIV 2207 GCCGGC G^CCGGC
NheI 474 GCTAGC G^CTAGC
NspV 741 TTCGAA TT^CGAA
SacII 838 CCGCGG CCGC^GG
SbfI 2763 CCTGCAGG CCTGCA^GG
SexAI 2990 ACCAGGT A^CC[AT]GGT
SfuI 741 TTCGAA TT^CGAA
SgrAI 2206 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2763 CCTGCAGG CCTGCA^GG
StuI 413 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 2200 GAATTC [GA]^AATT[CT]
3535 GAATTC
AfaI 146 GTAC GT^AC
1563 GTAC
AflIII 1359 ACATGT A^C[GA][CT]GT
1548 ACATGT
ApoI 2200 GAATTC [GA]^AATT[CT]
3535 GAATTC
AspI 811 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3541 GACAGTGTC
BbvCI 1467 GCTGAGG CCTCAGC(-5/-2)
3003 GCTGAGG
BspLU11I 1359 ACATGT A^CATGT
1548 ACATGT
EcoRI 2200 GAATTC G^AATTC
3535 GAATTC
HincII 560 GTCAAC GT[CT]^[GA]AC
1488 GTTGAC
HindII 560 GTCAAC GT[CT]^[GA]AC
1488 GTTGAC
MseI 1922 TTAA T^TAA
3245 TTAA
PaeR7I 2246 CTCGAG C^TCGAG
2899 CTCGAG
PflFI 811 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3541 GACAGTGTC
RsaI 146 GTAC GT^AC
1563 GTAC
SphI 464 GCATGC GCATG^C
1595 GCATGC
Tru9I 1922 TTAA T^TAA
3245 TTAA
Tth111I 811 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3541 GACAGTGTC
XhoI 2246 CTCGAG C^TCGAG
2899 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI